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The use of the extract of Artemisia annua in the prevention and treatment of Panax notoginseng black spot

A technology of Panax notoginseng black spot and extract, which is applied in the direction of chemicals, applications, and biocides for biological control, which can solve the problems of aggravated disease spread, high humidity, and high temperature, and achieve the suppression of Panax notoginseng black spot The effect of pathogenic bacteria

Active Publication Date: 2017-11-17
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The high temperature and high humidity in the Panax notoginseng garden shed, improper fertilization may aggravate the spread of the disease

Method used

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  • The use of the extract of Artemisia annua in the prevention and treatment of Panax notoginseng black spot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 : Preparation of South Artemisia Artemisiae Extract

[0019] Select the fresh stems and leaves of Artemisia miltiorrhiza without disease, wash them and dry them naturally. The fresh body is crushed and passed through a sieve with a pore size of 50 mesh, then the stem and leaf powder are mixed and placed in a dry container, and 8 times the amount of ethanol is added to soak for 4 days at room temperature, and then filtered with four layers of gauze to obtain the filtrate. Repeat the soaking operation of the filter residue with 6 times the amount of ethanol in the previous step. After repeating 2 times, combine the filtrates three times. The filtrate was decompressed and concentrated, and after being sterilized by ultraviolet light, it was then made to volume with sterile water to a crude drug concentration of 2.0 g·mL -1 .

Embodiment 2

[0020] Example 2 : Bacteriostasis of South Artemisia Artemisiae Extract

[0021] 2.1 Materials and reagents

[0022] Southern mugwort ethanol extract, sterile water, PDA medium. The strains tested: Alternariapanax Whetzel was provided by the Plant Pathology Laboratory of Agricultural College of Guangxi University.

[0023] 2.2 Method

[0024] The antibacterial effect of the extract of Artemisia argyi was determined by mycelial growth rate method. Measure the crude drug concentration to 2.0g·mL -1 6.75ml, 3.375ml, and 1.6875ml of the South Artemisia wormwood extract solution were respectively adjusted to 10ml with sterile water to prepare different concentrations of South Artemisia Artemisia test solutions, and 1ml of South Artemisia Artemisia test solutions with different concentrations were respectively taken , add 14ml of melted PDA medium to a 90mm petri dish to make a drug-containing plate, so that the final test concentration is 0.0900g·mL -1 , 0.0450g·mL -1 and 0...

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Abstract

The invention discloses the use of the extract of Artemisia miltiorrhiza for preventing and treating black spot of Panax notoginseng, and belongs to the technical field of plant disease and insect pest control. Wash the body and dry it naturally, crush the fresh body and sieve it, then mix the stem and leaf powder and place it in a dry container; (2) add ethanol to the powder obtained in step (1) for the first filtration, To obtain the filtrate, add ethanol to the obtained filter residue for second filtration, repeat the filtration 2 times, and combine the filtrate for 3 times; (3) Concentrate the filtrate obtained in step (2) under reduced pressure, use ultraviolet light to sterilize, and then use sterile water Constant volume can obtain the Artemisia annua extract of the present invention. The southern mugwort extract of the invention has a good antibacterial effect on the pathogenic bacteria of root rot of notoginseng.

Description

technical field [0001] The invention relates to the technical field of disease and insect pest control, in particular to the use of Artemisia mugwort extract for preventing and treating Panax notoginseng black spot. Background technique [0002] Southern mugwort (Artemisia verlotorum Lamotte), belonging to the genus Artemisia in the family Asteraceae, is also known as bitter wormwood and white wormwood. Because Artemisia plants are similar in appearance and smell, they can also be collectively referred to as "wormwood" or "artemisia". They are distributed in most of my country, Europe, Asia, and Africa. It is a perennial plant with a fragrance. The main root is slightly obvious, and there are many lateral roots. Stems solitary or few, 50-100 cm high, branches 5-8 cm long, obliquely attached to the stem. It can be seen everywhere in the wild and occupies an important position in Chinese traditional culture. Can be used as medicine, has anti-inflammatory, hemostatic effect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/12A01P3/00
Inventor 李良波向维黄荣韶马仲辉甘凤琼张平刚韦继光
Owner GUANGXI UNIV
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