Application of artemisia verlotorum extract to prevention and control of panax pseudoginseng black spot

A technology of Panax notoginseng black spot and extract, applied in the direction of chemicals, applications, biocides, etc. for biological control, can solve the problems of aggravated disease spread, high humidity, high temperature, etc., and achieve the suppression of Panax notoginseng black spot The effect of pathogenic bacteria

Active Publication Date: 2015-05-27
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The high temperature and high humidity in the Panax notoginseng garden shed, improper fertilization may aggravate the spread of the disease

Method used

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  • Application of artemisia verlotorum extract to prevention and control of panax pseudoginseng black spot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 : Preparation of South Artemisia Artemisiae Extract

[0019] Select the fresh stems and leaves of Artemisia miltiorrhiza without disease, wash them and dry them naturally. The fresh body is crushed and passed through a sieve with a pore size of 50 mesh, then the stem and leaf powder are mixed and placed in a dry container, and 8 times the amount of ethanol is added to soak for 4 days at room temperature, and then filtered with four layers of gauze to obtain the filtrate. Repeat the soaking operation of the filter residue with 6 times the amount of ethanol in the previous step, and after repeating it twice, combine the filtrates three times. The filtrate was decompressed and concentrated, and after being sterilized by ultraviolet light, it was then made to volume with sterile water to a crude drug concentration of 2.0 g·mL -1 .

Embodiment 2

[0020] Example 2 : Bacteriostasis of South Artemisia Artemisiae Extract

[0021] 2.1 Materials and reagents

[0022] Southern mugwort ethanol extract, sterile water, PDA medium. The strains tested: Alternaria panax Whetzel was provided by the Plant Pathology Laboratory of the Agricultural College of Guangxi University.

[0023] 2.2 Method

[0024] The antibacterial effect of the extract of Artemisia argyi was determined by mycelial growth rate method. Measure the crude drug concentration to 2.0g·mL -1 6.75ml, 3.375ml, and 1.6875ml of the South Artemisia wormwood extract solution were respectively adjusted to 10ml with sterile water to prepare different concentrations of South Artemisia Artemisia test solutions, and 1ml of South Artemisia Artemisia test solutions with different concentrations were respectively taken , add 14ml of melted PDA medium to a 90mm petri dish to make a drug-containing plate, so that the final test concentration is 0.0900g·mL -1 , 0.0450g·mL -1 ...

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Abstract

The invention discloses application of an artemisia verlotorum extract to prevention and control of panax pseudoginseng black spot, belonging to the technical fields of plant pest prevention and control. A preparation method of the artemisia verlotorum extract comprises the following steps of (1) naturally airing after cleaning fresh stems and leaves of disease-free artemisia verlotorum, sieving after smashing the fresh stems and leaves of the disease-free artemisia verlotorum, then, mixing the stem powder and the leaf powder, and placing the mixed powder in a dry vessel; (2) adding ethanol into the artemisia verlotorum powder obtained in the step (1) to filter for the first time to obtain a filtrate, adding ethanol into the obtained filter residue again to filter for the second time, repeatedly filtering twice, and then, mixing the filtrates obtained in three times; and (3) carrying out reduced-pressure condensation and ultraviolet sterilization on the filtrate obtained in the step (2), and then, keeping the constant volume by using sterile water to obtain the artemisia verlotorum extract. The artemisia verlotorum extract disclosed by the invention has a favorable antibacterial effect for pathogenic bacteria of panax pseudoginseng black spot.

Description

technical field [0001] The invention relates to the technical field of disease and insect pest control, in particular to the use of Artemisia mugwort extract for preventing and treating Panax notoginseng black spot. Background technique [0002] Southern mugwort (Artemisia verlotorum Lamotte), belonging to the genus Artemisia in the family Asteraceae, is also known as bitter wormwood and white wormwood. Because Artemisia plants are similar in appearance and smell, they can also be collectively referred to as "wormwood" or "artemisia". They are distributed in most of my country, Europe, Asia, and Africa. It is a perennial plant with a fragrance. The main root is slightly obvious, and there are many lateral roots. Stems solitary or few, 50-100 cm high, branches 5-8 cm long, obliquely attached to the stem. It can be seen everywhere in the wild and occupies an important position in Chinese traditional culture. Can be used as medicine, has anti-inflammatory, hemostatic effect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/12A01P3/00
Inventor 李良波向维黄荣韶马仲辉甘凤琼张平刚韦继光
Owner GUANGXI UNIV
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