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Application of camptothecin in preparation of medicine resisting to EB (epstein-barr) virus in lysis period

A technology of EB virus and camptothecin is applied in the application field of camptothecin in the preparation of EB virus drugs against lysis phase, and achieves the effects of good medicinal prospects, high EB virus replication efficiency and inhibition of EB virus replication effect.

Inactive Publication Date: 2015-06-03
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the application of camptothecin in the preparation of anti-lytic phase Epstein-Barr virus drug has not been reported yet

Method used

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  • Application of camptothecin in preparation of medicine resisting to EB (epstein-barr) virus in lysis period
  • Application of camptothecin in preparation of medicine resisting to EB (epstein-barr) virus in lysis period
  • Application of camptothecin in preparation of medicine resisting to EB (epstein-barr) virus in lysis period

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 of the present invention provides the application of camptothecin in the preparation of anti-lytic Epstein-Barr virus drugs.

[0052] Step S101, cultivation of ZKO-293 cells

[0053] ZKO-293 cells were cultured in 37°C, 5% carbon dioxide, pH7.2-7.4 environment, adding RPMI serum containing 10% fetal bovine serum and 100μg / ml hygromycin;

[0054] Step S102, Zta eukaryotic vector transfection

[0055] Hp-luciferase plasmid and p3xFLAG-Zta were co-transfected into ZKO-293 cells for transformation for 6 hours, and Hp-luciferase plasmid and p3xFLAG plasmid were co-transfected into ZKO-293 cells as a control to detect and judge luciferase Whether the eukaryotic vector is successfully transfected, select the target cells that are successfully transfected with Zta cDNA, and adjust the cell density to 5×10 5 -10×10 5 a / ml;

[0056] Step S103, adding camptothecin for treatment

[0057] Add camptothecin respectively in above-mentioned cell culture liquid, make the c...

Embodiment 2

[0073] Example 2 of the present invention provides the application of camptothecin in the preparation of anti-lytic Epstein-Barr virus drugs.

[0074] Step S201, cultivation of ZKO-293 cells

[0075] ZKO-293 cells were cultured in 37°C, 5% carbon dioxide, pH7.2-7.4 environment, adding RPMI serum containing 10% fetal bovine serum and 100μg / ml hygromycin, and adjusted the cell density to 5×10 5 -10×10 5 a / ml;

[0076] Step S202, Zta eukaryotic vector transfection

[0077] The Hp-luciferase plasmid and p3xFLAG-Zta were co-transfected into ZKO-293 cells for transformation, and the Hp-luciferase plasmid and p3xFLAG plasmid were co-transfected into ZKO-293 cells as a control;

[0078] Step S203, adding camptothecin for treatment

[0079] Add camptothecin respectively in above-mentioned cell culture liquid, make the concentration of camptothecin be 0.1 (embodiment 2-1), 0.2 (embodiment 2-2), 0.3 (embodiment 2-3), 0.5 (embodiment 2-3) 2-4), 0.7 (Example 2-5) and 0.9 (Example 2-6)...

Embodiment 3

[0091] Example 3 of the present invention provides the application of camptothecin in the preparation of a medicament for preventing cancer caused by Epstein-Barr virus infection.

[0092] According to the experimental results of Example 1 and Example 2, camptothecin has a role in inhibiting the immediate early EB virus in the lytic phase and inhibiting the early EB virus in the lytic phase, effectively inhibiting the combination of Zta protein and OriLyt in EB virus-infected cells, and inhibiting EB virus. Virus replication has a good effect on preventing cancers caused by Epstein-Barr virus infection, such as: nasopharyngeal carcinoma, Burkitt's lymphoma, Hodgkin's disease (HD), gastric cancer, thymoma, etc.

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Abstract

The invention relates to the field of antiviral drugs and in particular discloses application of camptothecin in preparation of a medicine resisting to an EB (epstein-barr) virus in a lysis period and application of camptothecin in preparation of a medicine used for preventing cancer caused by EB virus infection. According to the application, early-stage EB virus infected cells and immediate early-stage EB virus infected cells are constructed and are treated by the camptothecin. The application of the camptothecin in preparation of the medicine resisting to the EB virus in the lysis period has the beneficial effects that a new medical application of the camptothecin is explored, and a new application field is expanded; and combination between Zta protein of the EB virus in the lysis period and OriLyt can be obviously inhibited, so that the camptothecin has a good medicinal prospect.

Description

technical field [0001] The invention relates to the technical field of camptothecin application, in particular to the application of camptothecin in the preparation of anti-lytic Epstein-Barr virus drugs. Background technique [0002] Epstein-Barr virus is the only lymphocrypovirus (lymphocrypovirus) that can cause human infection among members of the Herpesviridae Gamma subfamily, and is an infectious agent that is widely distributed in the human population. Epstein-Barr virus has the characteristics of B lymphocytes, can establish stealth infection in B lymphocytes, stimulate cell proliferation and transformation, and mainly dissociate in the cytoplasm in the form of multi-copy circular DNA and integrate into the host chromosome. The stimulation of the signaling pathway can lead to the transformation of B lymphocytes into paddle cells, so that Epstein-Barr virus can produce infectious virus particles, and finally lead to the lysis and death of host cells. In recent years,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4745A61P31/22A61P35/00
Inventor 王蒲唐超邹军辉万晓春
Owner SHENZHEN INST OF ADVANCED TECH
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