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Wild cotton or semi-wild cotton mirid resistance identification method

A technology of semi-wild cotton and identification method, which is applied in the field of wild cotton or semi-wild cotton resistance identification to lycium bugs, can solve the problems of blank, less utilization of resistance internal attributes, etc., and achieves strong practicability and operability, identification stable results

Inactive Publication Date: 2015-06-24
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

Since the mirid bug was not the main pest in the past, and most of the research on resistance identification methods revolved around cultivated varieties, the use of the original ecological resistance properties of wild cotton or semi-wild cotton was less, or even blank.

Method used

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  • Wild cotton or semi-wild cotton mirid resistance identification method
  • Wild cotton or semi-wild cotton mirid resistance identification method
  • Wild cotton or semi-wild cotton mirid resistance identification method

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example

[0068] 1. Experimental method:

[0069] (1) The test was carried out in a net room (6 meters long, 2.5 meters wide, and 2.0 meters high), and the identified varieties were planted in the net room. 10 plants were planted for each material, and were repeated 3 times. 0.25m, the seedling stage can control cotton aphids 1-2 times;

[0070] (2) 80-100 mesh nylon nets are covered on the outside of the net to prevent the invasion of pests and natural enemies; (3) artificially inoculate the mixed colony of Lygus aeruginosa in the 10-12 leaf stage of cotton growth, 1 head per square meter, and the released The mirid bugs were collected from natural populations in the field and raised indoors with green beans, with strong vitality;

[0071] (4) Clean the cage 10 days before releasing the mirid bug, and spray phoxim 1500 times liquid in the net room to kill pests and natural enemies;

[0072] (5) 7-10 days after inoculation, investigate the damage degree of the upper 3 leaves of cotton...

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Abstract

The invention discloses a wild cotton or semi-wild cotton mirid resistance identification method. The method specifically comprises the steps that identified varieties are planted in a net house; the net house is covered with a nylon net ranging from 80 meshes to 100 meshes; apolygus lucorum mixed populations are inoculated manually during 10-12 leaf stage of cotton growing with the density being one per square meter; a cage is cleared ten days before mirids are released, and 1500-fold phoxim is sprayed in the net house; the damage degrees of all the identified varieties to three leaves at the top of each cotton plant, the total number of lateral branches of each cotton plant and the number of damaged lateral branches are investigated in seven to ten days after the apolygus lucorum mixed populations are inoculated; the leaf damaged index, the lateral branch damage rate and the lateral branch damage reducing rate are calculated; the resistance levels of the identified varieties are judged. By means of the method, interference of the factors such as other pests and natural enemies is eliminated, the identification result is stable and reliable, and the practicality and operability are high.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a method for identifying wild cotton or semi-wild cotton resistance to mirid bugs. Background technique [0002] Although transgenic insect-resistant cotton has effectively curbed the harm of cotton bollworm, it is still of great significance to cultivate natural insect-resistant cotton containing insect-resistant genes from cotton. In recent years, some scientific researchers are also working on the research of using wild cotton to transfer beneficial genes to upland cotton, but no valuable and stable germplasm has been found. [0003] my country has already obtained from Asian cotton, Bick's cotton, Thurber's cotton, Stoke's cotton, abnormal cotton, Steeti cotton, Somali cotton, Mexican semi-wild cotton, Baumer's cotton, broad-leaved cotton, fluffy cotton Cotton bollworm resistance was bred from wild species such as cotton, and cotton aphid resistance was b...

Claims

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Application Information

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IPC IPC(8): A01G7/00A01G13/00
CPCA01G7/00A01G13/00
Inventor 雒珺瑜崔金杰张帅吕丽敏王春义朱香镇李春花
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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