Formula of induction medium for efficiently inducing anther culture of japonica rice

A technology for inducing culture medium and culture medium, applied in the field of agricultural science, can solve the problems of unsatisfactory flower cultivation efficiency, small population of regenerated plants, selection, and utilization limitations.

Inactive Publication Date: 2015-06-24
中一农业科技(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The growth and development of rice is determined by its own genes and environmental factors. Due to the influence of medium components, material differences, and cultivation environments, the current flower cu

Method used

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  • Formula of induction medium for efficiently inducing anther culture of japonica rice
  • Formula of induction medium for efficiently inducing anther culture of japonica rice
  • Formula of induction medium for efficiently inducing anther culture of japonica rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Prepare the culture medium with the following formula:

[0016] KNO 3 2400mg / L, (NH 4 ) 2 SO 4 400mg / L, KH 2 PO 4 350mg / L, CaCl 2 ?2H 2 O 140mg / L, MgSO 4 ?7H 2 O 160mg / L, Na 2 -EDTA 32.5mg / L, FeSO 4 ?7H 2 O 24mg / L, MnSO 4 ?4H 2 O 3.9mg / L, ZnSO 4 ?7H 2 O 1.3mg / L, H 3 BO 3 1.4mg / L, KI 0.65mg / L, glycerol 6.5mg / L, Na 3 C 6 h 5 o 7 ?H 2 O 0.5mg / L, folic acid 0.5mg / L, glycine 3.0mg / L, alanine 2.5mg / L, lysine 2.0mg / L, vitamin B1 2.0mg / L, vitamin B6 1mg / L, niacin 1mg / L, 2-hydroxyethyltrimethylammonium chloride 250mg / L, sucrose 55g / L, plant gel 5.5g / L;

[0017] 2,4-D 4.0mg / L, NAA 4.0mg / L, complex phthalein nucleic acid 0.5mg / L, hydrolyzed protein 0.5g / L, activated carbon 2.75g / L, taurine 0.075mg / L, biotin 0.075mg / L L, phytosulfokinetin PSKα 30pmol / L, pH 5.9.

[0018] The japonica rice varieties Tongkejing and Heihuzijing were selected as anther donors for anther culture, and uninucleated late anthers were collected as test materials. After surface d...

Embodiment 2

[0020] Prepare the medium with the following formula (preferred concentration of alanine added):

[0021] KNO 3 2400mg / L, (NH 4 ) 2 SO 4 400mg / L, KH 2 PO 4 350mg / L, CaCl2 ?2H 2 O 140mg / L, MgSO 4 ?7H 2 O 160mg / L, Na 2 -EDTA 32.5mg / L, FeSO 4 ?7H 2 O 24mg / L, MnSO 4 ?4H 2 O 3.9mg / L, ZnSO 4 ?7H 2 O 1.3mg / L, H 3 BO 3 1.4mg / L, KI 0.65mg / L, glycerol 6.5mg / L, Na 3 C 6 h 5 o 7 ?H 2 O 0.5mg / L, folic acid 0.5mg / L, glycine 3.0mg / L, lysine 2.0mg / L, vitamin B1 2.0mg / L, vitamin B6 1mg / L, niacin 1mg / L, 2-hydroxychloride Ethyltrimethylammonium 250mg / L, sucrose 55g / L, vegetable gel 5.5g / L;

[0022] 2,4-D 4.0mg / L, NAA 4.0mg / L, complex phthalein nucleic acid 0.5mg / L, hydrolyzed protein 0.5g / L, activated carbon 2.75g / L, taurine 0.075mg / L, biotin 0.075mg / L L, phytosulfokinetin PSKα 30pmol / L, pH 5.9.

[0023] The added concentration of alanine is set in the following 10 types: 0; 0.5 mg / L; 1 mg / L; 1.5 mg / L; 2 mg / L; 3mg / L; 3.5 mg / L; L; 8 mg / L.

[0024] The two japonica ri...

Embodiment 3

[0026] Prepare the culture medium with the following formula (preferred coagulant):

[0027] KNO 3 2400mg / L, (NH 4 ) 2 SO 4 400mg / L, KH 2 PO 4 350mg / L, CaCl 2 ?2H 2 O 140mg / L, MgSO 4 ?7H 2 O 160mg / L, Na 2 -EDTA 32.5mg / L, FeSO 4 ?7H 2 O 24mg / L, MnSO 4 ?4H 2 O 3.9mg / L, ZnSO 4 ?7H 2 O 1.3mg / L, H 3 BO 3 1.4mg / L, KI 0.65mg / L, glycerol 6.5mg / L, Na 3 C 6 h 5 o 7 ?H 2 O 0.5mg / L, folic acid 0.5mg / L, glycine 3.0mg / L, alanine 2.5mg / L, lysine 2.0mg / L, vitamin B1 2.0mg / L, vitamin B6 1mg / L, niacin 1mg / L, 2-hydroxyethyltrimethylammonium chloride 250mg / L, sucrose 55g / L;

[0028] 2,4-D 4.0mg / L, NAA 4.0mg / L, complex phthalein nucleic acid 0.5mg / L, hydrolyzed protein 0.5g / L, activated carbon 2.75g / L, taurine 0.075mg / L, biotin 0.075mg / L L, phytosulfokinetin PSKα 30pmol / L, pH 5.9.

[0029] The coagulant is set to the following 2 types: plant gel 2.75g / L, agar 3.5g; agar 7g.

[0030] The two japonica rice varieties were also selected as anther donors, the operation me...

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Abstract

The invention provides a formula of an induction medium for efficiently inducing anther culture of japonica rice. The formula of the induction medium comprises the following components in certain proportions: KNO3, (NH4)2SO4, KH2PO4, CaCl2.2H2O, MgSO4.7H2O, Na2-EDTA, FeSO4.7H2O, MnSO4.4H2O, ZnSO4.7H2O, H3BO3, KI, glycerol, Na3C6H5O7.H2O, folic acid, glycine, alanine, lysine, vitamin B1, vitamin B6, niacin, 2-hydroxyethyl trimethyl ammonium chloride, sucrose, plant gel, 2,4-D, NAA, phthalocyanine complex nucleic acid, protolysate, activated carbon, taurine, biotin and plant sulfide kinetin alpha. The induction medium has the advantages that callus of anther of japonica rice can be efficiently induced, the browning can be lowered, and the culture efficiency of the anther of japonica rice can be substantially improved.

Description

technical field [0001] The invention relates to a japonica rice anther culture medium formula, in particular to a japonica rice anther culture medium formula which can significantly reduce browning and efficiently induce japonica rice anther culture, and belongs to the field of agricultural science and technology. Background technique [0002] Anthers are the male organs of plants, and anther culture is to use plant tissue culture technology to grow anthers that have developed to a certain stage. Inoculate on the medium through aseptic operation, change the developmental program of anthers under a series of induction conditions, undergo mitosis, form cell clusters after dedifferentiation, and then form callus, undergo redifferentiation and develop into complete haploid plants the process of. [0003] In 1964, Guha and Maheshwari, two botanists at the University of Delhi in India, discovered that the embryoids grown from the anthers of Datura pilosula they cultivated were ha...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 不公告发明人
Owner 中一农业科技(广州)有限公司
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