Chinese cabbage est‑ssr marker and its primers and applications

A Chinese cabbage, labeling technology, applied in the field of molecular biology, can solve the problem that the Chinese cabbage EST-SSR primer is not saturated
CN104774951BActive Publication Date: 2017-09-15VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
Publication Date
2017-09-15

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Abstract

The invention relates to Chinese cabbage EST-SSR (expressed sequence tag-simple sequence repeat) markers as well as primers and applications thereof. The invention discloses a Chinese cabbage EST-SSR marker BR-es10 and a primer thereof, a Chinese cabbage EST-SSR marker BR-es13 and a primer thereof, and a Chinese cabbage EST-SSR marker BR-es15 and a primer thereof. By carrying out amplification on the DNA of 24 Chinese cabbage varieties, and carrying out detection by using 6% denaturing polyacrylamide gel and / or 12% non-denaturing polyacrylamide gel electrophoresis, a situation that the amplified products of the three sets of EST-SSR primers are obvious in banding pattern, and good diversity is achieved among varieties is found, so that different varieties can be distinguished well, therefore, the markers and primers thereof can be applied to the identification of Chinese cabbage varieties and the construction of variety fingerprints.
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Description

technical field

[0001] The patent of the invention relates to Chinese cabbage EST-SSR markers and their primers and applications, in particular to three groups of Chinese cabbage EST-SSR markers and their primers and their application in the construction of variety fingerprints, belonging to the technical field of molecular biology. Background technique

[0002] The emergence of molecular markers has transformed the selection in breeding work from traditional phenotypic selection to genotype selection, greatly improving the accuracy and efficiency of selection. Therefore, molecular markers have become an important and indispensable part of breeding work and genetics research since the emergence of molecular markers. Tool of. Especially the emergence of PCR-based molecular markers such as RAPD (Williams et al., 1990), SSR (Akkaya et al., 1992), AFLP (Vos et al., 1995), etc., because of their simplicity, rapidity and high efficiency. , so that the development and utilization ...

Claims

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