Application of traditional Chinese medicine composition to preparation of monoamine oxidase MAO inhibitor

A technology of monoamine oxidase and composition, which is applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve problems such as limited application, and achieve the effect of highlighting substantive characteristics

Inactive Publication Date: 2015-07-22
臧孝国
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its application is limited because of the high blood pressure caused by "cheese reaction"

Method used

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  • Application of traditional Chinese medicine composition to preparation of monoamine oxidase MAO inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Take 20 g of barb thorns, 18 g of fennel, 12 g of Aina xianggen, 15 g of iron chopsticks, and 20 g of rhubarb root, add water to decoct for 2 times, and the amount of water added for the first time is 10 times the weight of the medicinal material, Decocting for 2 hours, adding water for the second time is 8 times the weight of the medicinal materials, decocting for 1 hour, combining the decoction, concentrating, drying, and pulverizing to obtain Chinese medicine composition granules. The concentration conditions are: 0.07MPa, 60-70°C When concentrated under reduced pressure to 65°C, the relative density was 1.30. The spray-drying conditions are: the inlet air temperature is 120°C, the outlet air temperature is 80°C, the material temperature is 90°C, the atomization pressure is 0.4 MPa, and the spray speed is 10ml / s.

Embodiment 2

[0016] Embodiment 2: Chinese medicine composition detects the inhibitory activity to MAO-A and MAO-B with the method of fluorescent probe, described method is carried out according to the following steps:

[0017] 1. Test drug: Prepare the test drug according to the method in Example 1 above.

[0018] 2. Method: Dissolve the sample to be tested in DMSO, and make a series of samples with concentration gradient (1g crude drug amount / ml-10g crude drug amount / ml). Take 4ul of MAO (10mg / ml), boric acid buffer (PH=8.4), 50ul of BSA (50mg / ml), add the sample solution to be tested, mix well, react in a water bath at 30-38°C for 3h, then add probe 7 -(3-Aminopropoxy)coumarin 2ul (50mmol / ml), the final concentration of the inhibitor is 0~10-2mmol / l, and the mixture is reacted in a water bath at 30±5°C for 12h, and then tested with a fully functional fluorescence spectrophotometer (λex / λem=365 / 460nm) for detection. IC50 was calculated according to the obtained data, and the results are...

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Abstract

The invention provides application of a traditional Chinese medicine composition to preparation of a monoamine oxidase (MAO) inhibitor. The traditional Chinese medicine composition is prepared from 20 parts of delavay raspberry, 18 parts of galium verum, 12 parts of blumea balsamifera roots, 15 parts of wintersweet roots or stems and 10 parts of roots of multibract raspberry. Compared with the conventional monoamine oxidase MAO inhibitor, the monoamine oxidase MAO inhibitor provided by the invention has the advantages that the inhibitory activity and the selectivity on MAO-A and MAO-B of the monoamine oxidase MAO inhibitor are both greatly improved, that is, a great improvement is achieved.

Description

technical field [0001] The invention relates to a new application of a Chinese medicine composition, in particular to an application of the Chinese medicine composition in preparing monoamine oxidase MAO inhibitors. Background technique [0002] Since the anti-tuberculosis drug isoniazid was found to be a potential MAO inhibitor, research on MAO inhibitors has become a hot topic. Iproniazid became the first MAO inhibitor, because it was discovered that this kind of MAO inhibitor containing hydrazine structure can also inactivate P450s and cause liver toxicity; and studied the non-hydrazine MAO inhibition represented by tranylcypromine Agents, this category can be called the second generation. But because "cheese reaction" causes high blood pressure, its application is limited. Therefore, it is quite necessary and urgent to develop a newer and more efficient monoamine oxidase inhibitor. Contents of the invention [0003] The object of the present invention is to provide ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61P43/00
Inventor 臧孝国
Owner 臧孝国
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