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Rapid propagation method for flos farfarae

A fast technology for coltsfoot flower, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low reproduction efficiency of coltsfoot flower rhizome and limit the rapid development of coltsfoot flower industry, and achieve good application prospects and cycle time Short, fast growing effect

Inactive Publication Date: 2015-08-19
秦孜昌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problem that the rhizome reproduction efficiency of coltsfoot is low, which limits the rapid development of coltsfoot industry, and provides a rapid breeding method for coltsfoot, which is suitable for the breeding of coltsfoot seedlings and promotes the large area of ​​coltsfoot Large-scale cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] In the late autumn and early winter seasons, choose coltsfoot rhizomes that are thick and multi-flowered, white in color, and free from diseases and insect pests, wash the attached soil with clean water, add a few drops of Tween 80 to 0.1% mercury solution and soak for 10 minutes, and then use Rinse with sterile water three times, each time for 2min, cut into small sections of 1cm as explants, inoculate the explants in the induction medium and carry out cluster bud induction culture under light conditions to obtain cluster buds; the induction medium is MS The medium was used as the basic medium, and the macroelements were halved, and sucrose at a concentration of 15g / L, agar at a concentration of 2g / L, 6-BA at a concentration of 0.5mg / L, and NAA at a concentration of 0.3mg / L were added. The clustered buds are transferred to the proliferation medium to carry out the subculture of the clustered buds under light conditions to obtain the subcultured proliferation of the clus...

Embodiment 2

[0019] In the late autumn and early winter seasons, choose coltsfoot rhizomes that are thick and multi-flowered, white in color, and free from diseases and insect pests, wash the attached soil with clean water, add a few drops of Tween 80 to 0.1% mercury solution and soak for 10 minutes, and then use Rinse with sterile water three times, each time for 5min, cut into small sections of 2cm as explants, inoculate the explants in the induction medium and carry out cluster bud induction culture under light conditions to obtain cluster buds; the induction medium is MS The medium was used as the basic medium, and the macroelements were halved, and 25 g / L sucrose, 5 g / L agar, 1.5 mg / L 6-BA, and 1 mg / L NAA were added. The clustered buds are transferred to the proliferation medium and carried out the subculture of the clustered buds under light conditions to obtain the subsequent proliferation of the clustered buds; the proliferation medium uses MS (1 / 3NH4N03) medium as the basic medium,...

Embodiment 3

[0021] In the late autumn and early winter seasons, choose coltsfoot rhizomes that are thick and multi-flowered, white in color, and free from diseases and insect pests, wash the attached soil with clean water, add a few drops of Tween 80 to 0.1% mercury solution and soak for 10 minutes, and then use Rinse with sterile water three times, each time for 3 minutes, cut into small sections of 1-2 cm as explants, inoculate the explants in the induction medium and carry out cluster bud induction culture under light conditions to obtain cluster buds; the induction medium MS medium was used as the basic medium, macronutrients were halved, and sucrose at a concentration of 20g / L, agar at a concentration of 3.5g / L, 6-BA at a concentration of 1.2mg / L, and 0.6mg / L were added The NAA. The clustered buds are transferred to the proliferation medium to carry out the subculture of the clustered buds under light conditions to obtain the subcultured proliferation of the clustered buds; the proli...

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Abstract

The invention discloses a rapid propagation method for flos farfarae. The rapid propagation method comprises four steps: multiple-shoot induction of stem tips, multiple-shoot subculture multiplication, rooting culture and test-tube plantlets transplantation. The flos farfarae seedlings obtained by the method are high in survival rate of transplantation; and bud seedlings are healthy and fast in growth. The method disclosed by the invention is short in cycle and low in cost, can completely meet the requirements of large-scale and large-scale cultivation of the flos farfarae, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of raising seedlings of Chinese medicinal materials, and in particular relates to a rapid breeding method of coltsfoot flowers. Background technique [0002] Coltsfoot (Tussilago farfara), also known as winter flower, butterbur or coltsfoot dandelion, belongs to the genus Coltsfoot in the Asteraceae family. The flower buds are used as medicine, with pungent and warm nature and flavor, which has the functions of moistening the lungs, reducing phlegm and relieving cough. It is recorded in "The Classic": "It is most suitable for drinking, coughing, coughing, and cold-shrinking lung meridian." Although the smell is warm, moist but not dry, the evil of warm and heat, which is stagnated in the lung meridian and cannot be dredged, can also be cured. Therefore, it can be used for cough caused by exogenous diseases and internal injuries, cold and heat, deficiency and excess. Especially chronic cough due to lung de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 秦孜昌
Owner 秦孜昌
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