A kind of method of tissue culture of Vaccinium camphora with stem segment as explant

Abstract

The invention discloses a method for culturing Vaccinium camphoriae tissue with stem segments as explants, comprising the following steps: step 1: culture medium configuration, using WPM as the basic medium, and adding NAA and TDZ; step 2: stem segments For callus induction, take Vaccinium camphoria tissue culture seedlings with strong stems, remove the leaves in an aseptic operating table, cut the stems into long stems, and scratch the surface and place them in the storage room configured in step 1. Cultivate in the culture medium; Step 3: Adventitious bud induction culture, grow into seedlings induced by step 2 into the adventitious bud induction medium and carry out adventitious bud induction culture; Step 4: Rooting induction of Vaccinium camphora leaf tissue culture seedlings, Inoculate the robust Vaccinium camphoria plantlets grown from the adventitious bud induction culture in step 3 into the rooting medium to induce rooting to obtain rooted Vaccinium camphoria plantlets. The method of the invention has short period and low cost, can completely meet the demand of large-scale cultivation, and has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture of vaccinia camphoria with stem segments as explants. Background technique [0002] Camphor leaf vaccinia (original variety) V. dunalianumWight. is an evergreen shrub of the Rhododendronaceae Vaccinium genus, also known as rice fruit (Kunming, Yunnan) and long-tailed vaccinia (Illustrated Handbook of Higher Plants in China). Vaccinium camphor leaves leathery or thick leathery, apex tail-like acuminate; assembly tidbits, axillary; berries spherical, purple-black when ripe, covered with white powder. Distributed in Sichuan, Guizhou, Yunnan, and Tibet in China, often growing in hillside shrubs, under broad-leaved forests or lime hill shrubs, and abroad in Sikkim, Bhutan, India (northeast), Myanmar (northeast) to Vietnam ( Flora of China). As the raw material of Quezui tea, Vaccinium camphora has not yet been artificially planted...

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Problems solved by technology

[0003] Vaccinium camphor is widely used as the raw material of Quezui tea and the main plant natural whitening agent of skin care products. However, at present, the breeding cycle of Vaccinium camphor is long, and it is impossible to effectively obtain a large number of Vaccinium camphora seedlings in a short period of time.

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