Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!
Tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of tissue culture medium and culture medium, which is applied in tissue culture, microorganisms, plant regeneration, etc., can solve the problems of not meeting production and market demand, plant trait variation, and low survival rate of cuttings, and achieve good application prospects and high rooting rate. High, high survival rate effect
Inactive Publication Date: 2013-06-05
CHONGQING UNIV OF ARTS & SCI
View PDF4 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Due to the large variation in plant traits of this variety, clonal breeding methods such as cuttings and grafting are usually used to propagate excellent single-plant clones. However, there are not many rootstocks and scion resources for grafting, and the survival rate of cuttings is lower than 40%, resulting in a low reproductive coefficient. , cannot meet production and market demand
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0024] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings. The explant No. 1 of Lonicerae cinerea Yulei used in the examples was provided by the greenhouse of the Flower Research Institute of Chongqing University of Arts and Science.
[0025] 1. Optimization of callus induction medium
[0026] Rinse the No. 1 explant of Lonicerae pilosula Yulei (the leaves extracted in the same year) with tap water, rinse with 70% ethanol solution for 30 seconds, and then rinse with 0.1% HgCl 2 Disinfect the solution for 8 minutes, and finally rinse with sterile water 4 to 5 times to thoroughly wash away residual HgCl 2 . Cut the sterilized leaves into 1cm 3 size, inoculated on callus induction medium. The callus induction medium used MS, MB and B5 as the basic medium, and added 6-BA 1mg / L, KT 2mg / L, NAA 0.1mg / L, carrageenan 6g / L and s...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts. The medium comprises at least one of the following mediums: a callus induction medium which comprises B5, 2mg / L of 6-BA (6-benzylaminopurine), 2mg / L of KT (kinetin), 0.1mg / L of NAA (naphthylacetic acid), 5.5-6.0g / L of carragheenan and 30g / L of sucrose and has a pH value of 5.6-5.8; an adventitious bud differentiation medium which comprises B5, 1mg / L of 6-BA, 2mg / L of KT, 0.1mg / L of NAA, 5.5-6.0g / L of carragheenan and 30g / L of sucrose and has a pH value of 5.6-5.8; an adventitious bud subculture medium which comprises MB, 1mg / L of 6-BA, 0.8mg / L of IAA (indoleacetic acid), 5.5-6.0g / L of carragheenan and 30g / L of sucrose and has a pH value of 5.6-5.8; and a rooting medium which comprises 1 / 3MB, 1.5mg / L of IBA (indolebutyric acid), 0.1mg / L of NAA, 5.5-6.0g / L of carragheenan and 20g / L of sucrose and has a pH value of 5.6-5.8. The medium of the invention, which has the advantages of high callusinductivity, high dventitious bud differentiation rate and propagation coefficient, high rooting rate, high transplanting survival rate of test tube sprouts, robust sprout growth, normal leaf shape and leaf color, rapid subcutaneous rooting and new leaf germination, and the like, can satisfy needs of the large-scale cultivation of Lonicera macranthoides Hand. Mazz Yulei No.1, and has a good application prospect.
Description
technical field [0001] The invention belongs to the technical field of plant tissue culture and relates to a tissue culture medium for plant seedlings. Background technique [0002] Gray felted honeysuckle (Lonicera m acranthoides Hand. Mazz) is a perennial semi-evergreen winding small shrub or erect small shrub plant of Lonicera genus Lonicera. The content of chlorogenic acid in its flower buds is the highest among all kinds of honeysuckle in the country. One of the main varieties of commercial honeysuckle. [0003] Yulei No. 1 was approved by the Chongqing Forest Variety Approval Committee in 2008 as a new variety of Lonicera aureus. The flower yield of this variety is more than 400 kg per mu, which is 30-80% higher than that of the traditional planting of Lonicera chinensis. Its stress resistance and adaptability are also stronger than that of traditional home-grown Lonicera spp. ~30 days and the entire flowering period is full of flower buds, basically no flowering. Co...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.