Factorized breeding method of blueberry tissue-cultured seedlings
An industrialized, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticultural tools/equipment, etc., to achieve the effects of fast growth, large-scale cultivation, and good application prospects
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[0015] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
[0016] The present invention takes the Cuguashan blueberry in Yongchuan District, Chongqing City as the experimental material, systematically carries out research work such as rooting culture and test-tube seedling transplantation, and establishes and optimizes the blueberry tissue culture seedling factory breeding method.
[0017] 1. Optimization of rooting medium
[0018] The sprouts growing vigorously and with a plant height of 3-5 cm from the subcultured proliferating clustered buds were transferred to five rooting media with different hormone concentration ratios, and the rooting culture was carried out under light conditions to obtain test-tube plantlets; the rooting media was 1 / 4MW, 1 / 2MW, 1 / 4CQM6, and 1 / 2CQM6 medium are basic medium (the formula of basic medium is shown in Table 1-4).
[0019] Table 1 rooting medium with 1 / 4MW ...
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