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Factorized breeding method of blueberry tissue-cultured seedlings

An industrialized, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticultural tools/equipment, etc., to achieve the effects of fast growth, large-scale cultivation, and good application prospects

Inactive Publication Date: 2014-01-29
CHONGQING UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many studies on the propagation and tissue culture of blueberry varieties, but there are few studies on the induced rooting of unrooted seedlings

Method used

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  • Factorized breeding method of blueberry tissue-cultured seedlings
  • Factorized breeding method of blueberry tissue-cultured seedlings
  • Factorized breeding method of blueberry tissue-cultured seedlings

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Embodiment Construction

[0015] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

[0016] The present invention takes the Cuguashan blueberry in Yongchuan District, Chongqing City as the experimental material, systematically carries out research work such as rooting culture and test-tube seedling transplantation, and establishes and optimizes the blueberry tissue culture seedling factory breeding method.

[0017] 1. Optimization of rooting medium

[0018] The sprouts growing vigorously and with a plant height of 3-5 cm from the subcultured proliferating clustered buds were transferred to five rooting media with different hormone concentration ratios, and the rooting culture was carried out under light conditions to obtain test-tube plantlets; the rooting media was 1 / 4MW, 1 / 2MW, 1 / 4CQM6, and 1 / 2CQM6 medium are basic medium (the formula of basic medium is shown in Table 1-4).

[0019] Table 1 rooting medium with 1 / 4MW ...

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Abstract

The invention discloses a factorized breeding method of blueberry (LM3) tissue-cultured seedlings, which is characterized by comprising the step of taking 1 / 4 MW culture medium as rooting medium, wherein the medium does not contain any hormone including IBA (indolebutyric acid) and NAA (naphthaleneacetic acid). Through the factorized breeding method of the blueberry tissue-cultured seedlings, the seedling rooting rate is up to 100%, and the root systems are strong and have lateral buds, the transplanting survival rate is high and the seedlings are strong and rapid in growth; and the method disclosed by the invention is short in period, low in cost and capable of completely satisfying the demand of large-area cultivation and has favorable application prospect.

Description

technical field [0001] The invention relates to a seedling breeding method, which is an industrial breeding method of blueberry tissue culture seedlings. Background technique [0002] Blueberry (Blueberry) is a shrub berry fruit tree of Ericaceae (Ericaceae) Vaccinium.SPP. It is one of the main small fruit fruit trees in the world at present. It is widely used in industries such as pigment and food processing. In addition to the organic acids, vitamins and minerals contained in ordinary fruits, blueberry fruit is also rich in unsaturated fatty acids, ellagic acid and a variety of biologically active substances. It is low in sugar and fat, and its antioxidant capacity ranks first among all fruits and vegetables. . Therefore, it is listed as one of the top five healthy foods by the International Food and Agriculture Organization, and it can be called the "King of Fruits in the World". [0003] my country's blueberry introduction work began in the mid-1980s, and the scale of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄科刘奕清汤玲黄登艳袁海英唐建民陈泽雄廖林正
Owner CHONGQING UNIV OF ARTS & SCI
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