Tissue-culturing and rapid-propagating method of dragon fruits

A dragon fruit group, technology of dragon fruit, applied in the field of plant propagation, to achieve the effect of a large number of roots, strong roots, and strong buds

Inactive Publication Date: 2015-09-16
吴华球
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rapid propagation of seedlings by in vitro culture has been widely used on most horticultural plants, but there are still few reports about the in vitro culture technology research of dragon fruit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The stem section of the pitaya is used as an explant, and the proliferation culture, elongation culture and rooting culture are respectively carried out. The method of proliferation culture is as follows: first wet the explants with 70% alcohol, sterilize with 0.1% mercuric chloride for 10 minutes, rinse repeatedly with sterile water for 15 minutes each time, and explant the rinsed pitaya The explants were pre-cultured on 1 / 2MS medium for 1 week; the pre-cultured pitaya explants were cultured in medium A, the culture temperature was controlled at (25±2)°C, and the light time was 12h·d -1 , light intensity 2000lx, culture 28-32d to obtain pitaya budlets; wherein, the above medium A is the medium obtained after adding 6-BA and IAA on the MS medium, the pH is 5.5, and the concentration of 6-BA is 0.8mg·L -1 , the concentration of IAA is 0.3mg·L -1 .

[0020] The specific method of elongation culture is: cut off the small buds of dragon fruit cultured by proliferation fr...

Embodiment 2

[0023] The stem section of the pitaya is used as an explant, and the proliferation culture, elongation culture and rooting culture are respectively carried out. The method of proliferation culture is as follows: first wet the explants with 70% alcohol, sterilize with 0.1% mercuric chloride for 10 minutes, rinse repeatedly with sterile water for 5 times, each time for 10 minutes, and explant the washed dragon fruit The explants were pre-cultured on 1 / 2MS medium for 1 week; the pre-cultured pitaya explants were cultured in medium A, the culture temperature was controlled at (25±2)°C, and the light time was 12h·d -1 , light intensity 2000lx, cultivated for 28-32d to obtain small buds of pitaya; wherein, the above medium A is the medium obtained after adding 6-BA and IAA on the MS medium, the pH is 5.8, and the concentration of 6-BA is 1.2mg·L -1 , the concentration of IAA is 0.5 mg·L -1 .

[0024] The specific method of elongation culture is: cut off the small buds of dragon f...

Embodiment 3

[0027] The stem section of the pitaya is used as an explant, and the proliferation culture, elongation culture and rooting culture are respectively carried out. The method of proliferation culture is as follows: first wet the explants with 70% alcohol, sterilize with 0.1% mercuric chloride for 6-10 minutes, rinse repeatedly with sterile water for 5 times, each time for 10-15 minutes, and wash the explants The pitaya explants were first pre-cultured on 1 / 2MS medium for 1 week; the pre-cultured pitaya explants were placed in medium A for cultivation, the culture temperature was controlled at (25±2)°C, and the light time 12h·d -1 , light intensity 2000lx, cultivated for 30d to obtain small buds of pitaya; wherein, the above medium A is the medium obtained after adding 6-BA and IAA on the MS medium, the pH is 5.6, and the concentration of 6-BA is 1.0mg · L -1 , the concentration of IAA is 0.4mg·L -1 .

[0028] The specific method of elongation culture is: cut off the small bud...

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PUM

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Abstract

The invention discloses a tissue-culturing and rapid-propagating method of dragon fruits, and in particular relates to the technical field of plant propagation. According to the invention, dragon fruit stems are used as explants; multiplication culture, elongation culture and rooting culture are respectively carried out. A multiplication culture method comprises the following steps of wetting the explants by using 70% alcohol, sterilizing and washing, and putting the washed dragon fruit explants on a 1/2 MS culture medium to pre-culture for one week; putting the pre-cultured dragon fruit explants in a culture medium A to culture for 28-32 d so as to obtain dragon fruit sprouts, wherein the culture temperature is controlled within (25+/-2) DEG C; the illumination time is 12h.d<-1>; and the illumination intensity is 20001x. According to the invention, the dragon fruit sprouts are robust; regeneration seedlings are rapid in growth speed; the rooting number is high; generated roots are robust; the tissue-culturing and rapid-propagating method is a rapid-propagating technology of dragon fruits; raw materials used in the invention are wide in source and low in application cost; and industrialized, scale and intensive production of dragon fruit seedlings can be realized easily.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to a method for tissue culture and rapid propagation of dragon fruit. Background technique [0002] Dragon fruit (Hylocereus undatus Britt & Rose) has another name called red dragon fruit, sesame fruit, fairy honey fruit, green dragon fruit, etc., and is a fruit cultivar of the genus Liangtianchi in the cactus family. The plant is native to the tropical regions of Central America, and its flowers and fruits are edible, and it is a local traditional food and an important economic crop. It was introduced to Taiwan from the Americas in the late 1980s and early 1990s. Dragon fruit is high in fiber, low in calories, rich in vitamins B1, B2, B3, C, glucose, minerals, and various trace elements. [0003] In recent years, the southern China region has introduced species one after another, and the planting area has continued to expand. At present, cuttings or grafting are mostly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 吴华球
Owner 吴华球
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