Rapid propagation method for virus-free seedlings of passion fruits

A technology for virus-free seedlings and passion fruit, applied in horticultural methods, botanical equipment and methods, gardening and other directions, can solve the problems of flooding of inferior seedlings, low seedling reproduction efficiency, loss of growers, etc. Time-consuming breeding and improved quality

Inactive Publication Date: 2018-08-24
广西君朗农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main problem in the passion fruit planting industry is the cultivation of seedlings. The lack of strict market supervision at this stage has led to a large number of low-quality seedlings flooding them, causing huge losses to growers; traditional methods of cultivating passion fruit seedlings are divided into two types, one is Cutting and rooting of passion fruit branches is the seedlings; the other is to sow passion fruit seeds to obtain seedlings and then graft them. The above-mentioned traditional methods often have many problems, such as cuttings and grafting scions with viruses, resulting in seedlings with viruses, In addition, the rooting of traditional soil cuttings is slow, the root system is weak, the seedlings are weak, and the seedling reproduction efficiency is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A rapid breeding method for passion fruit virus-free seedlings, comprising the following steps:

[0024] S1: Collect passion fruit shoot tip tissue and carry out tissue culture under sterile environment, the medium used in the tissue culture process is MS medium, and in the medium, add mixed phytohormones at a concentration of 15ppm, bring its root Obtain virus-free seedlings when growing to 2cm; the mixed phytohormone is made by mixing NAA:IBA according to the mass ratio of 2.5:1;

[0025] S2: To domesticate and transplant the virus-free seedlings, first open the mouth of the bottle and exercise for 2 days, then take out the plants, then wash the root culture medium and transplant it into a substrate containing nutrient solution for cultivation, and obtain the first-generation virus-free seedlings; Nutrient solution is calculated per liter of solution: containing 900mg calcium nitrate tetrahydrate, 450mg potassium nitrate, 60mg ammonium nitrate, 100mg potassium dihydro...

Embodiment 2

[0030] A rapid breeding method for passion fruit virus-free seedlings, comprising the following steps:

[0031] S1: Collect the shoot tip tissue of passion fruit and carry out tissue culture in a sterile environment. The medium used in the tissue culture process is MS medium, and a mixed phytohormone is added to the medium at a concentration of 20ppm, and the roots are taken When growing to 3cm, the virus-free seedlings are obtained; the mixed plant hormone is made by mixing NAA:IBA according to the mass ratio of 3:1;

[0032] S2: To domesticate and transplant the virus-free seedlings, first open the mouth of the bottle and exercise for 3 days, then take out the plants, then wash the root culture medium and transplant it into a substrate containing nutrient solution for cultivation, and obtain the first-generation virus-free seedlings; The nutrient solution is calculated per liter of solution: containing 945mg calcium nitrate tetrahydrate, 490mg potassium nitrate, 75mg ammoniu...

Embodiment 3

[0037] A rapid breeding method for passion fruit virus-free seedlings, comprising the following steps:

[0038] S1: Collect the shoot tip tissue of passion fruit and carry out tissue culture in a sterile environment. The medium used in the tissue culture process is MS medium, and in the medium, a mixed plant hormone is added at a concentration of 18ppm, and the roots are taken Obtain virus-free seedlings when growing to 2.5cm; The mixed phytohormone is made by mixing NAA:IBA according to the mass ratio of 2.8:1;

[0039] S2: To domesticate and transplant the virus-free seedlings, first open the mouth of the bottle and exercise for 2.5 days, then take out the plants, then wash the root culture medium and transplant it into a substrate containing nutrient solution for cultivation, and obtain the first-generation virus-free seedlings The nutrient solution is calculated by every liter of solution: containing 930mg calcium nitrate tetrahydrate, 475mg potassium nitrate, 68mg ammoniu...

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PUM

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Abstract

The invention provides a rapid propagation method for virus-free seedlings of passion fruits and belongs to the field of agricultural planting. The rapid propagation method comprises the steps: firstly, collecting stem tip tissues of the passion fruits, and carrying out tissue culture in an aseptic environment to obtain virus-free seedlings; then, domesticating, transplanting and cultivating the virus-free seedlings to obtain first-generation virus-free seedlings; colleting stem segments of the first-generation virus-free seedlings, carrying out rapid propagation by using an aerosol cultivation bed, and carrying out reasonable management to rapidly obtain second-generation virus-free seedlings; and next, cultivating the second-generation virus-free seedlings in a farmland, then, picking branches of second-generation plants, cultivating the branches on the aerosol cultivation bed to form a finished product of commercial seedlings. The invention provides the rapid propagation method forthe virus-free seedlings of the passion fruits; and due to the combination of a plant tissue culture technology and a plant non-test-tube rapid propagation technology in the method, the virus-free passion fruit seedlings in the true sense are cultivated, meanwhile, the seedling culture efficiency is increased, the quality of seedlings is improved, and the propagation industrialization of the passion fruit seedlings is promoted.

Description

【Technical field】 [0001] The invention relates to the technical field of agricultural planting, in particular to a rapid breeding method for virus-free passion fruit seedlings. 【Background technique】 [0002] Passion fruit, also known as egg fruit, is rich in 17 kinds of amino acids, multivitamins, carotenoids and various trace elements needed by the human body. Passion fruit can relieve headaches caused by anxiety, tension, depression and nervous tension , Stomach pain, frequent urination, palpitation, muscle spasms, convulsions, pain and other discomforts, enhance the body's resistance, improve immunity, and improve the composition of intestinal flora, and play a role in protecting the stomach from absorbing harmful substances. Purify the body, avoid the deposition of harmful substances in the body, and then achieve the effect of improving the skin and beautifying the appearance. The advantages in these aspects make the market demand of passion fruit multiply day by day. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李永辉滕传姮
Owner 广西君朗农业科技有限公司
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