Rapid propagation method for virus-free seedlings of passion fruits
A technology for virus-free seedlings and passion fruit, applied in horticultural methods, botanical equipment and methods, gardening and other directions, can solve the problems of flooding of inferior seedlings, low seedling reproduction efficiency, loss of growers, etc. Time-consuming breeding and improved quality
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Embodiment 1
[0023] A rapid breeding method for passion fruit virus-free seedlings, comprising the following steps:
[0024] S1: Collect passion fruit shoot tip tissue and carry out tissue culture under sterile environment, the medium used in the tissue culture process is MS medium, and in the medium, add mixed phytohormones at a concentration of 15ppm, bring its root Obtain virus-free seedlings when growing to 2cm; the mixed phytohormone is made by mixing NAA:IBA according to the mass ratio of 2.5:1;
[0025] S2: To domesticate and transplant the virus-free seedlings, first open the mouth of the bottle and exercise for 2 days, then take out the plants, then wash the root culture medium and transplant it into a substrate containing nutrient solution for cultivation, and obtain the first-generation virus-free seedlings; Nutrient solution is calculated per liter of solution: containing 900mg calcium nitrate tetrahydrate, 450mg potassium nitrate, 60mg ammonium nitrate, 100mg potassium dihydro...
Embodiment 2
[0030] A rapid breeding method for passion fruit virus-free seedlings, comprising the following steps:
[0031] S1: Collect the shoot tip tissue of passion fruit and carry out tissue culture in a sterile environment. The medium used in the tissue culture process is MS medium, and a mixed phytohormone is added to the medium at a concentration of 20ppm, and the roots are taken When growing to 3cm, the virus-free seedlings are obtained; the mixed plant hormone is made by mixing NAA:IBA according to the mass ratio of 3:1;
[0032] S2: To domesticate and transplant the virus-free seedlings, first open the mouth of the bottle and exercise for 3 days, then take out the plants, then wash the root culture medium and transplant it into a substrate containing nutrient solution for cultivation, and obtain the first-generation virus-free seedlings; The nutrient solution is calculated per liter of solution: containing 945mg calcium nitrate tetrahydrate, 490mg potassium nitrate, 75mg ammoniu...
Embodiment 3
[0037] A rapid breeding method for passion fruit virus-free seedlings, comprising the following steps:
[0038] S1: Collect the shoot tip tissue of passion fruit and carry out tissue culture in a sterile environment. The medium used in the tissue culture process is MS medium, and in the medium, a mixed plant hormone is added at a concentration of 18ppm, and the roots are taken Obtain virus-free seedlings when growing to 2.5cm; The mixed phytohormone is made by mixing NAA:IBA according to the mass ratio of 2.8:1;
[0039] S2: To domesticate and transplant the virus-free seedlings, first open the mouth of the bottle and exercise for 2.5 days, then take out the plants, then wash the root culture medium and transplant it into a substrate containing nutrient solution for cultivation, and obtain the first-generation virus-free seedlings The nutrient solution is calculated by every liter of solution: containing 930mg calcium nitrate tetrahydrate, 475mg potassium nitrate, 68mg ammoniu...
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