Tissue culture method for dendrobium moniliforme

A technique for tissue culture of Dendrobium candidum, applied in the field of cultivation, can solve problems such as resource destruction and extinction of Dendrobium candidum, and achieve the effects of being beneficial to rooting, promoting rooting, and promoting the growth of seedlings

Inactive Publication Date: 2016-03-30
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Abstract
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Problems solved by technology

[0003] However, because the natural reproduction of Orchidaceae seeds mainly relies on branch reproduction, it is difficult to germinate in the wild. With the development of society, people's excessive picking has caused most of the wild Dendrobium copper-skinned resources to be destroyed and almost extinct. Therefore, looking for A method of tissue culture cultivates Dendrobium copper-skinned to make Dendrobium copper-skinned rapid propagation, realize the industrialization of high-quality seedlings of Dendrobium copper-skinned seedlings, meet people's needs, and become an urgent problem to be solved by people

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  • Tissue culture method for dendrobium moniliforme

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Embodiment 1

[0034] Step 1, take the fruit pods of Dendrobium copper-skinned dendrobium and carry out disinfection and pretreatment, after soaking 5 minutes with the aqueous solution of liquid detergent of 2% by volume fraction, wash 15 minutes with running water, then put the fruit pods of Dendrobium copper-skinned noble dendrobium in a sterile ultra-clean bench The pods were clamped with sterile tweezers, and the entire surface of the pods was stained with 75% alcohol by volume, and then burned. After the alcohol was burned out, the burning was repeated once, each burning for 5 seconds, to obtain explants.

[0035] Step 2. Cut the explants, take out the seeds and inoculate them into the first medium, and cultivate them in the first medium for 50 days at a culture temperature of 23° C., a light intensity of 1500 lux, and a light time of 8 h / d , to obtain sterile test tube seedlings.

[0036] The first medium includes: MS, 0.5 mg / L gibberellin, 2.0 g / L activated carbon, 5 g / L agar and 30 g...

Embodiment 2

[0046] Step 1, take the fruit pods of Dendrobium copper-skinned dendrobium and carry out disinfection and pretreatment, soak 5 minutes with the volume fraction of 2% detergent solution, rinse with running water for 30 minutes, and then put the fruit pods of Dendrobium copper-skinned dendrobium in a sterile ultra-clean bench The pods were clamped with sterile tweezers, and the entire surface of the pods was stained with 75% alcohol by volume, and then burned. After the alcohol was burned out, the burning was repeated for 10 seconds each time to obtain explants.

[0047] Step 2: Cut the explants, take out the seeds and inoculate them into the first culture medium, and culture them in the first culture medium for 70 days at a culture temperature of 27°C, a light intensity of 1500 lux, and a light time of 10 h / d , to obtain sterile test tube seedlings.

[0048] The first medium includes: MS, 0.5 mg / L gibberellin, 2.0 g / L activated carbon, 5 g / L agar and 30 g / L sucrose, and the ini...

Embodiment 3

[0058] Step 1, take the fruit pods of Dendrobium copper-skinned dendrobium and carry out disinfection and pretreatment, soak 5 minutes with the aqueous solution of detergent liquid that is 2% by volume fraction, rinse 20 minutes with running water, then put the fruit pods of Dendrobium copper-skinned noble dendrobium in the sterile ultra-clean bench The pods were clamped with sterile tweezers, and the entire surface of the pods was stained with 75% alcohol by volume, and then burned. After the alcohol was burned out, the burning was repeated once, each burning for 8 seconds, to obtain explants.

[0059] Step 2: Cut the explants, take out the seeds and inoculate them into the first culture medium, and cultivate them in the first culture medium for 60 days at a culture temperature of 25°C, a light intensity of 1500 lux, and a light time of 9 h / d , to obtain sterile test tube seedlings.

[0060] The first medium includes: MS, 0.5 mg / L gibberellin, 2.0 g / L activated carbon, 5 g / L ...

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Abstract

The invention discloses a tissue culture method for dendrobium moniliforme. The method comprises the steps that 1, dendrobium moniliforme pods are taken to serve as explants to be disinfected; 2, the explants germinate to obtain sterile test-tube plantlets; 3, the sterile test-tube plantlets are cultured to obtain test-tube plantlet cluster buds; 4, the test-tube plantlet cluster buds are placed in a strong plantlet culture medium to be cultured in the strong plantlet culture medium to obtain test-tube plantlets; 5, the test-tube plantlets are placed in a rooting culture medium to obtain tissue culture seedlings; 6, the tissue culture seedlings are transplanted into a matrix to be cultured after seedling hardening is performed. According to the method, the multiplication coefficient of the dendrobium moniliforme cluster buds obtained through the culture method reaches 20-30 times, healthy and strong high-quality dendrobium moniliforme seed seedlings can be supplied in the short time, and the scale seedling culturing problem of the dendrobium moniliforme is effectively solved.

Description

technical field [0001] The present invention relates to a cultivation method, more specifically, the present invention relates to a kind of tissue culture method of Dendrobium copper-skinned. Background technique [0002] Dendrobium copper bark, also known as: thin-stem dendrobium, thin yellow grass, belongs to the genus Dendrobium orchidaceae, a perennial epiphytic herb, is a precious Chinese herbal medicine with high medicinal and health care value. In recent years, the study of Dendrobium has aroused people's widespread interest. Dendrobium copper contains high polysaccharides, total alkaloids and other chemical components. Eye, anti-tumor, anti-aging, anti-radiation, enhancing the body's immunity and other effects, so Dendrobium copper skin has gradually attracted people's attention. [0003] However, because the natural reproduction of Orchidaceae seeds mainly relies on branching, it is difficult to germinate in the wild. With the development of society, people's exces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王晓峰李刚缪剑华农东新韦坤华韦莹王一诺
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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