Asparagus cochinchinensis tissue culture seedling culture medium
A culture medium and asparagus group technology, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., to achieve the effects of shortening disinfection time, reducing production costs, and simple operation
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[0082] Described asparagus tissue culture medium, its preparation method comprises the following steps:
[0083] S1: After peeling the watermelon, crush the fruit with a grinder, and pass the obtained juice through a 3000-5000 mesh sieve to obtain fruit juice;
[0084] S2: The fruit juice, potassium salt, phosphorus salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, iron salt, vitamin, amino acid obtained in step S1 , indole butyric acid, gibberellin, 6-benzyl adenine, naphthalene acetic acid, kinetin, cosolvent, dispersant, compatibilizer, antibacterial agent, water mix, the microwave power is 80-120W, the temperature is Stir at 62-68°C for 30-50min at a rotational speed of 200-400r / min to prepare the mixed solution A;
[0085] S3: Add a conditioner and a stabilizer to the mixed solution A prepared in step S2, and stir for 15-25 minutes at a temperature of 46-52° C. and a rotational speed of 10...
Embodiment 1
[0088] A culture medium for asparagus tissue culture seedlings, in parts by weight, comprising the following raw materials: 250 parts of fruit juice, 3 parts of potassium salt, 2.5 parts of phosphorus salt, 1.8 parts of nitrogen salt, 0.9 part of calcium salt, 0.8 part of magnesium salt, 0.4 part of iodine salt, 0.3 part of boron salt, 0.2 part of molybdenum salt, 0.3 part of zinc salt, 0.2 part of manganese salt, 0.1 part of copper salt, 0.1 part of iron salt, 0.13 part of vitamin, 0.14 part of amino acid, 0.08 part of indole butyric acid, 0.08 parts of gibberellin, 0.07 parts of 6-benzyl adenine, 0.08 parts of naphthaleneacetic acid, 0.05 parts of kinetin, 0.15 parts of conditioner, 2.2 parts of cosolvent, 1.8 parts of dispersant, 1.5 parts of compatibilizer, and 1.2 parts of stabilizer 2.2 parts of antibacterial agent, 3000 parts of water;
[0089] The fruit juice is watermelon juice;
[0090] The potassium salt is potassium chloride;
[0091] The phosphorus salt is calci...
Embodiment 2
[0116] A culture medium for asparagus tissue culture seedlings, in parts by weight, comprising the following raw materials: 120 parts of fruit juice, 2.2 parts of potassium salt, 1.3 parts of phosphorus salt, 1.1 part of nitrogen salt, 0.7 part of calcium salt, 0.6 part of magnesium salt, 0.3 part of iodine salt, 0.2 part of boron salt, 0.2 part of molybdenum salt, 0.3 part of zinc salt, 0.1 part of manganese salt, 0.1 part of copper salt, 0.1 part of iron salt, 0.09 part of vitamin, 0.08 part of amino acid, 0.05 part of indole butyric acid, 0.06 parts of gibberellin, 0.04 parts of 6-benzyl adenine, 0.06 parts of naphthaleneacetic acid, 0.03 parts of kinetin, 0.17 parts of conditioner, 1.4 parts of cosolvent, 1.2 parts of dispersant, 1 part of compatibilizer, and 0.9 parts of stabilizer 1.5 parts of antibacterial agent, 2500 parts of water;
[0117] The fruit juice is watermelon juice;
[0118] The potassium salt is potassium chloride;
[0119] The phosphorus salt is calcium...
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