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Asparagus cochinchinensis tissue culture seedling culture medium

A culture medium and asparagus group technology, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., to achieve the effects of shortening disinfection time, reducing production costs, and simple operation

Active Publication Date: 2017-02-08
张家港和智珅知识产权有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a medium for asparagus tissue culture seedlings to solve the problems of how to increase the content of asparagine, the main active ingredient of asparagus, and improve economic benefits in the existing tissue culture medium

Method used

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  • Asparagus cochinchinensis tissue culture seedling culture medium

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preparation example Construction

[0082] Described asparagus tissue culture medium, its preparation method comprises the following steps:

[0083] S1: After peeling the watermelon, crush the fruit with a grinder, and pass the obtained juice through a 3000-5000 mesh sieve to obtain fruit juice;

[0084] S2: The fruit juice, potassium salt, phosphorus salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, iron salt, vitamin, amino acid obtained in step S1 , indole butyric acid, gibberellin, 6-benzyl adenine, naphthalene acetic acid, kinetin, cosolvent, dispersant, compatibilizer, antibacterial agent, water mix, the microwave power is 80-120W, the temperature is Stir at 62-68°C for 30-50min at a rotational speed of 200-400r / min to prepare the mixed solution A;

[0085] S3: Add a conditioner and a stabilizer to the mixed solution A prepared in step S2, and stir for 15-25 minutes at a temperature of 46-52° C. and a rotational speed of 10...

Embodiment 1

[0088] A culture medium for asparagus tissue culture seedlings, in parts by weight, comprising the following raw materials: 250 parts of fruit juice, 3 parts of potassium salt, 2.5 parts of phosphorus salt, 1.8 parts of nitrogen salt, 0.9 part of calcium salt, 0.8 part of magnesium salt, 0.4 part of iodine salt, 0.3 part of boron salt, 0.2 part of molybdenum salt, 0.3 part of zinc salt, 0.2 part of manganese salt, 0.1 part of copper salt, 0.1 part of iron salt, 0.13 part of vitamin, 0.14 part of amino acid, 0.08 part of indole butyric acid, 0.08 parts of gibberellin, 0.07 parts of 6-benzyl adenine, 0.08 parts of naphthaleneacetic acid, 0.05 parts of kinetin, 0.15 parts of conditioner, 2.2 parts of cosolvent, 1.8 parts of dispersant, 1.5 parts of compatibilizer, and 1.2 parts of stabilizer 2.2 parts of antibacterial agent, 3000 parts of water;

[0089] The fruit juice is watermelon juice;

[0090] The potassium salt is potassium chloride;

[0091] The phosphorus salt is calci...

Embodiment 2

[0116] A culture medium for asparagus tissue culture seedlings, in parts by weight, comprising the following raw materials: 120 parts of fruit juice, 2.2 parts of potassium salt, 1.3 parts of phosphorus salt, 1.1 part of nitrogen salt, 0.7 part of calcium salt, 0.6 part of magnesium salt, 0.3 part of iodine salt, 0.2 part of boron salt, 0.2 part of molybdenum salt, 0.3 part of zinc salt, 0.1 part of manganese salt, 0.1 part of copper salt, 0.1 part of iron salt, 0.09 part of vitamin, 0.08 part of amino acid, 0.05 part of indole butyric acid, 0.06 parts of gibberellin, 0.04 parts of 6-benzyl adenine, 0.06 parts of naphthaleneacetic acid, 0.03 parts of kinetin, 0.17 parts of conditioner, 1.4 parts of cosolvent, 1.2 parts of dispersant, 1 part of compatibilizer, and 0.9 parts of stabilizer 1.5 parts of antibacterial agent, 2500 parts of water;

[0117] The fruit juice is watermelon juice;

[0118] The potassium salt is potassium chloride;

[0119] The phosphorus salt is calcium...

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Abstract

The invention discloses an asparagus cochinchinensis tissue culture seedling culture medium, which is prepared from the following raw materials: a fruit juice, kali salt, phosphor salt, nitrogen salt, calcium salt, magnesium salt, iodate, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, ferric salt, vitamin, amino acid, indolebutyric acid, gibberellin, 6-BA, naphthylacetic acid, kinetin, a conditioner, a cosolvent, a dispersing agent, a compatilizer, a stabilizer, an antibacterial agent and water. By adopting the asparagus cochinchinensis tissue culture seedling culture medium provided by the invention for tissue-culturing asparagus cochinchinensis, the obtained seedling quality is good, the survival rate is high, and the effective constituent asparagine is high.

Description

technical field [0001] The invention belongs to the technical field of asparagus planting, and in particular relates to a culture medium for asparagus tissue culture seedlings. Background technique [0002] Asparagus is a commonly used traditional Chinese medicine, which has the effects of nourishing yin and promoting body fluid, moistening the lungs and clearing the heart. In recent years, the demand for wild asparagus in the market has increased significantly, resulting in the destruction of wild asparagus, so the artificial cultivation technology of asparagus has gradually developed. Since most of the asparagus currently on the market are scattered and planted by farmers, and the varieties are relatively miscellaneous, it is difficult to guarantee the content of asparagine, the active ingredient of asparagus. Contents of the invention [0003] The invention provides a medium for asparagus tissue culture seedlings to solve the problems of how to increase the content of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 韦波
Owner 张家港和智珅知识产权有限公司
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