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A kind of detoxification tissue culture matrix and method suitable for cut flower chrysanthemum heat treatment stem tip

A technology for stem tip detoxification and cut-flower chrysanthemum, which is applied in the field of plant cultivation and cultivation, can solve the problems of inefficient large-scale production of seedlings, degradation of cut-flower chrysanthemum varieties, and affecting the quality of cut flowers, so as to promote large-scale production and improve the quality of seedlings. Good, high incidence of cluster buds

Active Publication Date: 2021-08-10
苏州胜景泰园林环境建设有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the current deficiencies in the degeneration of cut chrysanthemums and purple wild chrysanthemums, and the transplanting and planting technology of tissue cultured seedlings, the purpose of this invention is to provide a method for improving the efficient proliferation of detoxified tissue cultured seedlings suitable for heat treatment of cut chrysanthemums, and to solve the problem of Long-term asexual reproduction leads to a large accumulation of viruses in the plant, causing the degeneration of cut chrysanthemum varieties and weakening of growth potential, affecting the quality of cut flowers, and the problem that seedlings cannot be efficiently mass-produced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1:

[0048] The first object of the present invention is to form a method for efficient breeding of cut-flower chrysanthemum heat-treated stem tip detoxified tissue-cultured seedlings. The implementation site is the garden flower genetics and breeding laboratory of the Institute of Horticulture, Zhejiang Academy of Agricultural Sciences and the cut-flower chrysanthemum production base of Jiaxing Jiade Horticultural Co., Ltd., the above-mentioned The method includes the following steps:

[0049] (1) The preparation of the culture medium: including basic medium and the components of the culture medium at each stage of tissue culture, and the weight of each component per liter is:

[0050] a) Basic medium: where,

[0051] Shoot tip induction medium Ⅰ is 20g / L sucrose, 4.4 g / L agar powder, 1 / 2 B5+Ca(NO3) at pH 5.8 2 500mg / L medium;

[0052] Ordinary medium (explant primary induction, strong seedling medium and shoot tip induction medium II) is MS medium with 30 g...

Embodiment 2

[0071] The first object of the present invention is to form a method for efficient breeding of cut-flower chrysanthemum heat-treated stem tip detoxified tissue-cultured seedlings. The implementation site is the garden flower genetics and breeding laboratory of the Institute of Horticulture, Zhejiang Academy of Agricultural Sciences and the cut-flower chrysanthemum production base of Jiaxing Jiade Horticultural Co., Ltd., the above-mentioned The method includes the following steps:

[0072] (1) The preparation of the culture medium: including basic medium and the components of the culture medium at each stage of tissue culture, and the weight of each component per liter is:

[0073] a) Basic medium: where,

[0074] Shoot tip induction medium Ⅰ is 20g / L sucrose, 4.4 g / L agar powder, 1 / 2 B5+Ca(NO3) at pH 5.8 2 500mg / L medium;

[0075] Ordinary medium (explant primary induction, strong seedling medium and shoot tip induction medium II) is MS medium with 35 g / L sucrose, 4.4 g / L a...

Embodiment 3

[0094] In this example: the domesticated varieties of purple wild chrysanthemum were collected in Chun'an, Zhejiang Province and carried out tissue culture in Hangzhou. In mid-April 2017, 200 bottles of rooted tissue culture seedlings with a height of 7-10 cm were selected, with an average of 10 plants per bottle, and were carried out in Hangzhou Greenhouse. Transplanting test.

[0095] Specific operation steps: Wash the tissue-cultured seedlings with clean water, drain the water, and air-dry naturally. Use medicaments (50% carbendazim WP 600 times, 97% hymexazol 1700-2000 times, 80% mancozeb WP 400-600 times, 40% azoxystrobin 800-1000 times) disinfection configuration good substrate. The dried tissue-cultured seedlings were inserted into the treated peat substrate for cutting culture. After 1-2 months of growth, cut off the 7-10cm shoots and dip them in rooting powder. Mix the rooting coconut peat matrix with disinfectants (50% carbendazim WP 600 times, 80% mancozeb WP 400...

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Abstract

The invention discloses a detoxification tissue culture medium suitable for heat treatment of chrysanthemum stem tips and a method thereof, belonging to the technical field of plant cultivation. The tissue culture medium is composed of a combination of induction medium and a combination of induction medium for detoxification shoot tips which are used sequentially in stages , strong seedling culture and proliferation medium combination, rooting medium combination composition: the induction medium combination is composed of explant induction medium, explant first-generation induction medium, and explant induction medium used in sequence; detoxification The shoot tip induction medium combination is composed of explant induction medium, shoot tip induction medium I, shoot tip induction medium II used in sequence, strong seedling culture and proliferation medium combination: it includes strong seedlings used in sequence Culture medium and proliferation medium; the invention solves the problem that due to long-term asexual reproduction, a large amount of viruses accumulate in the plant body, causing the degeneration of chrysanthemum varieties and weakening of growth potential, affecting the quality of cut flowers, and at the same time, the problem that seedlings cannot be produced efficiently and on a large scale.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, in particular to a detoxification tissue culture substrate and method suitable for heat treatment of cut chrysanthemum stem tips. Background technique [0002] At present, the cut chrysanthemums cultivated in our province are mainly summer chrysanthemums and autumn chrysanthemums. The harvest period of summer chrysanthemums is from June to July. The growth characteristics are in a warm environment, with a night temperature of 10°C-23°C and a day temperature of 23°C-30°C. Under the environment, it can show good growth characteristics of the variety, is not sensitive to photoperiod response, and can be cultivated under natural light, but it is not resistant to high temperature. When cultivated under high temperature in summer, it is easy to have thickened calyx, smaller flower diameter, curled and uneven petals, and even The phenomenon of inability to develop and bloom after budding, repr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 郭方其吴超袁峰黑银秀柴金甫刘君丁晓瑜徐智豪黎侠
Owner 苏州胜景泰园林环境建设有限公司
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