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Rapid propagation method of orchid

An orchid and rapid technology, applied in the field of rapid propagation of orchids, can solve the problems of poor orchid quality, low survival rate, unscientific orchid planting, etc., and achieve the effect of improving poor quality and improving survival rate.

Inactive Publication Date: 2015-09-23
柳州市长林苗木种植专业合作社
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AI Technical Summary

Problems solved by technology

[0004] However, the current orchid planting is not scientific enough, resulting in poor quality and low survival rate of orchids

Method used

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Embodiment Construction

[0014] The invention discloses a method for rapid propagation of orchids, which comprises the following steps:

[0015] Preparation of asexually propagated leaves Select the buds on the bulbs of orchid plants as explants, scrub them with detergent and rinse with tap water for 40-50 minutes, then disinfect the surface, and finally rinse with sterile water, put MS+5.0mg / L 6-benzylaminoadenine + 50g / L sucrose + 7g / L agar, pH 5.5 culture medium to induce seedlings, culture conditions: 18-22 ℃, light intensity 1200-1400Lux, photoperiod: 13h / d, when the seedlings grow to 2-3 leaves and the leaves are 6-7cm long, use scissors to cut the leaves into 0.9-1.1cm long leaves for later use;

[0016] Using MS medium as the basic medium, add 8.0-10.0mg / L 6-benzylaminoadenine, 0.1-0.3mg / L a-naphthaleneacetic acid (NAA for short), 30g / L sucrose and 7g / L agar to make a differentiation medium with a pH of 5.6. The culture conditions are: 17-23°C, light intensity of 1000-1500Lux, photoperiod of ...

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Abstract

The invention provides a rapid propagation method of orchid. The rapid propagation method includes steps of selecting buds on corm of an orchid plant as explant, brushing and cleaning the explant by cleanser essence, washing by tap water for 40-50 min, disinfecting the surface and finally flushing with sterile water; putting the explanted into PH 5.5 culture medium with MS, 5.0 mg / L of 6-benzylamino adenine, 50 g / L of cane sugar and 7 g / L of agar to culture and induce into seedling under the conditions of 18-22 degrees Centigrade, 1200-1400 Lux of illumination intensity and 13 h / d of photoperiod, cutting leaves into 0.9-1.1 cm long by scissors until the seedling grows 6-7 cm long with two to three leaves for reserve. The rapid propagation method has the advantages that orchid can be quickly propagated, quality of orchid can be improved and survival rate is increased.

Description

technical field [0001] The invention relates to the technical field of planting, in particular to a method for rapid propagation of orchids. Background technique [0002] Orchid refers to the general term for the entire Orchidaceae. In my country, the traditional concept of "orchid" only refers to the Orchidaceae and Cymbidium plants that are produced in my country. Orchidaceae is a class of precious plants with high ornamental value and economic value. [0003] The orchids in the traditional Chinese famous flowers only refer to several species of ground orchids distributed in the Chinese orchids, such as Chunlan, Huilan, Jianlan, Molan and Hanlan, which are usually referred to as "Chinese orchids". This type of orchid is quite different from tropical orchids with large and colorful flowers. They have no eye-catching beauty, no huge flowers and leaves, but they have a simple, quiet, elegant and clean temperament, which is very in line with the aesthetic standards of orient...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 冯世娇
Owner 柳州市长林苗木种植专业合作社
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