A kind of method extracting Coprinus comatus element from Coprinus comatus

A technology for the mushrooms of chicken leg mushrooms, which is applied in the field of biomedicine, can solve the problems of no research on the extraction of chicken leg mushrooms, and achieve the effects of shortening the production cycle, simple and coherent process, and high effective components.

Active Publication Date: 2018-08-07
湖南杰萃生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, reports on Coprinus comatus at home and abroad mainly focus on protein, polysaccharides, etc., and there is no research on the extraction of Coprinus comatus in Coprinus comatus.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 10kg of dried Coprinus comatus, put it into a 200L extraction tank, add 100L of 0.1% hydrochloric acid (mass fraction, the same below) solution, and start stirring; open the jacket and steam to heat to 50-60°C, start timing, and extract for 1 hour; The feed liquid is passed through a 400-mesh filter bag, and 100 L of 0.1% hydrochloric acid solution is put into the filter residue again, and the extraction is repeated for 1 hour; When the pH value reaches 7.2, centrifuge; add citric acid to the centrifuged liquid to adjust the pH value to 5.3, and centrifuge; the centrifuged supernatant passes through a 25L AB-8 resin column at a flow rate of 25L / h; The impurities that are not adsorbed by the resin were eluted at a flow rate of 50L / h, and then 25L of 25% ethanol was used for elution at a flow rate of 10L / h, and then 25L of 75% ethanol was used for desorption at a flow rate of 10L / h. Press and recover the ethanol desorption solution, concentrate until the solid conte...

Embodiment 2

[0030] Weigh 300kg of dried Coprinus comatus, put it into a 3000L extraction tank, add 2400L of 0.1% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 50-60°C, start timing, and extract for 1 hour; the feed liquid passes through a 400-mesh filter bag , and put 2400L of 0.1% hydrochloric acid solution into the filter residue again, and repeat the extraction for 1h; Add citric acid to the centrifugal supernatant to adjust the pH value to 5.3, and centrifuge; the centrifugal supernatant passes through a 1000L AB-8 resin column at a flow rate of 1000L / h; Remove impurities that are not adsorbed by the resin, then elute with 1000L of 25% ethanol at a flow rate of 500L / h, then desorb with 1500L of 80% ethanol at a flow rate of 500L / h, collect the alcohol desorption solution, recover the ethanol desorption solution under reduced pressure and concentrate it to The solid content reaches more than 50%, and vacuum-dried to obtain 9.3kg of dry extract, an...

Embodiment 3

[0032] Weigh 200kg of dried Coprinus comatus, put it into a 3000L extraction tank, add 1600L of 0.1% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 50-60°C, start timing, and extract for 1 hour; the feed liquid passes through a 400-mesh filter bag , and put 1200L of 0.1% hydrochloric acid solution into the filter residue again, and repeat the extraction for 1h; Add citric acid to the centrifugal supernatant to adjust the pH value to 5.3, and centrifuge; the centrifugal supernatant passes through a 1000L AB-8 resin column at a flow rate of 1000L / h; Remove impurities that are not adsorbed by the resin, then use 1000L of 25% ethanol to elute at a flow rate of 500L / h, then desorb with 1500L of 75% ethanol at a flow rate of 500L / h, collect the alcohol desorption solution, recover the ethanol desorption solution under reduced pressure and concentrate it to The solid content reaches more than 50%, and vacuum-dried to obtain 6.1kg of dry extract, ...

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PUM

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Abstract

The invention discloses a method for extracting Coprinus comatus from Coprinus comatus. The method comprises the following steps: (1) extracting Coprinus comatus at 50-60°C with a hydrochloric acid solution with a mass fraction of 0.05-0.2% to obtain an extract; (2) adding alkali to the extract to adjust the pH value to 7.0-7.3, and then separating the solid and liquid to remove insoluble matter to obtain a clear aqueous solution A; (3) adding acid to the aqueous solution A to adjust the pH value to 5.0-5.5 , and then solid-liquid separation to remove insolubles, to obtain a clear aqueous solution B; (4) the aqueous solution B is added to the macroporous adsorption resin column, and the macroporous adsorption resin is washed with water to remove unadsorbed impurities; then the volume fraction Wash the macroporous adsorption resin with 25-30% ethanol solution to remove impurities with higher polarity; then use 75-85% ethanol solution as desorption solution to wash the macroporous adsorption resin, collect the desorption solution after washing, and Concentrate under reduced pressure and dry to obtain Coprinus comatus extract. The method has reasonable process, high extraction efficiency, simple operation and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting Coprinus comatus from Coprinus comatus, and relates to the field of biomedicine. Background technique [0002] Coprinus comatus (Coprinus comatus), the scientific name of Coprinus pubescens, belongs to the phylum Fungi, Basidiomycotina, Phylomycetes, Agaricaceae, Coprinus family, and Coprinus genus in taxonomy. Coprinus comatus has tender meat, delicious taste and rich nutrition. It is sweet and smooth in taste, beneficial to the spleen and stomach, clears the heart and calms the nerves, and regular consumption can help digestion, increase appetite and treat hemorrhoids. Modern studies have shown that Coprinus comatus has anti-oxidation, lowering blood sugar, preventing liver damage, anti-tumor, improving immunity, and antibacterial effects. One of the 16 rare edible fungi in one. [0003] Coprinus comatus is the main active ingredient in Coprinus comatus. Coprinus comatus can effectively inhibit non...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/07A23L33/00
Inventor 黄六仔
Owner 湖南杰萃生物技术有限公司
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