Method for extracting agaricus bisporus polysaccharide from agaricus bisporus

Abstract

The invention discloses a method for extracting agaricus bisporus polysaccharide from agaricus bisporus. The method comprises the following steps of (1) extracting agaricus bisporus by a hydrochloric acid solution with the mass fraction being 0.25 to 0.35 percent for 1 to 3h at 30 to 40 DEG C to obtain an extracting solution; (2) adding alkali into the extracting solution to regulate the pH value to be 7.5 to 8.0, and then performing solid-liquid separation to remove insoluble substances to obtain a clear water solution A; (3) adding acid into the water solution A to regulate the pH value to be 4.0 to 4.5, and then performing solid-liquid separation to remove insoluble substances to obtain a clear water solution B; (4) adding the water solution B into a macroporous absorption resin column; firstly using water to wash macroporous absorption resin to remove impurities which are not adsorbed; then using an ethanol solution with the volume fraction being 15 to 20 percent to wash the macroporous absorption resin to remove impurities with high polarity; next using an ethanol solution with the volume fraction being 65 to 70 percent as deabsorption liquid to wash the macroporous absorption resin, collecting the washed deabsorption liquid, and performing vacuum concentration and drying to obtain agaricus bisporus extracts. The method has the advantages that the process is reasonable; the extraction efficiency is high; the operation is simple; the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting Tricholoma polysaccharide from Tricholoma, and relates to the field of biomedicine. Background technique [0002] Tricholoma bisporus, scientific name Agaricus bisporus (Agaricus bisporus), Tricholoma bisporus, round mushroom, mushroom, Agaricus bisporus, white mushroom, belongs to Fungi, Basidiomycotina, Basidiomycetes, Agaricaceae, Agaricaceae, and Mushrooms. [0003] The protein content of Agaricus bisporus is 35%-38%. It contains 6 kinds of amino acids necessary for the human body. It is also rich in vitamin B1, vitamin B2, vitamin PP, nucleotides, niacin, ascorbic acid and vitamin D. Its nutritional value is vegetable and 4-12 times that of fruit, enjoying the reputation of "health food" and "king of vegetables". It is favored by the domestic market, especially the international market. [0004] Tricholoma has very good anti-tumor activity, and the anti-tumor activity of the polysaccharide contain...

AI Technical Summary

Problems solved by technology

[0005] At present, domestic and foreign reports on Tricholoma mainly focus on proteins, and there are few studies on the extraction of Tricholoma polysaccharides from Tricholoma.