Method of extracting Agrocybe aegerita polysaccharide from Agrocybe aegerita

The technology of tea tree mushroom polysaccharide and tea tree mushroom is applied in the field of biomedicine, which can solve the problems of less extraction research, and achieve the effects of high extraction efficiency, good extraction consistency and high content of active ingredients.

Inactive Publication Date: 2016-01-27
GUANGXI NANNING SHENGQIAN TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the reports on tea tree mushroom at home and abroad mainly focus on proteins, and there are few studies on the extraction of tea tree polysaccharides from tea tree mushroom

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 100 kg of dried tea tree mushroom and crush it to less than 0.5 cm, put it into the extraction tank, add 500 L of 0.3% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 40 ° C, start timing, extract for 3 hours, and filter the liquid through 400 mesh bag; after the reflux extraction is completed, the feed liquid is passed through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 7.5, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 5.0, and centrifuged; Add the flow rate to the 100L macroporous adsorption resin column, first wash the macroporous adsorption resin with water at a flow rate of 120L / h to remove unadsorbed impurities; then wash the macropores with a volume fraction of 15% ethanol solution at a flow rate of 45L / h Adsorption resin removes the higher impurity of polarity; Then use volume fraction as 65% ethanol solution to wash macropor...

Embodiment 2

[0032] Weigh 100 kg of dried tea tree mushroom and crush it to less than 0.5 cm, put it into the extraction tank, add 300 L of 0.25% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 30 ° C, start timing, extract for 2 hours, and filter the liquid through 400 mesh bag; after the reflux extraction is completed, the feed liquid passes through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 8.0, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 4.5, and centrifuged; Add the flow rate to the 100L macroporous adsorption resin column, first wash the macroporous adsorption resin with water at a flow rate of 100L / h to remove unadsorbed impurities; then wash the macropores with a volume fraction of 15% ethanol solution at a flow rate of 30L / h Adsorption resin removes the higher impurity of polarity; Then use volume fraction as 70% ethanol solution to wash macroporou...

Embodiment 3

[0034] Weigh 100kg of dried tea tree mushroom and crush it to less than 0.5cm, put it into the extraction tank, add 400L of 0.35% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 35°C, start timing, extract for 1 hour, and filter the liquid through 400 mesh bag; after the reflux extraction is completed, the feed liquid passes through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 7.8, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 4.8, and centrifuged; Add the flow rate to the 100L macroporous adsorption resin column, first wash the macroporous adsorption resin with water at a flow rate of 200L / h to remove unadsorbed impurities; then wash the macroporous resin with a volume fraction of 20% ethanol solution at a flow rate of 50L / h Adsorption resin removes the higher impurity of polarity; Then use volume fraction as the ethanol solution of 65% as desorpt...

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Abstract

The invention a method of extracting Agrocybe aegerita polysaccharide from Agrocybe aegerita, comprising following steps: (1), extracting Agrocybe aegerita at 30-40 DEG C for 1-3 h with hydrochloric acid solution having a mass fraction of 0.25-0.35% to obtain extract; (2), adding a base to the extract to adjust pH to 7.5-8.0, and removing insoluble substances by solid-liquid separation to obtain clarified aqueous solution A; (3), adding an acid to the aqueous solution A to adjust pH to 4.0-4.5, and removing insoluble substances by solid-liquid separation to obtain clarified aqueous solution B; (4), adding the aqueous solution B to macroporous resin columns, removing unadsorbed impurities by washing the macroporous resin column with water; washing macroporous resin with ethanol solution having a volume fraction of 15-20% to remove impurities higher in polarity; using ethanol solution having a volume fraction of 65-70% as a desorption solution to wash the macroporous resin, collecting the desorption solution after washing, and performing reduced pressure concentration and drying to obtain Agrocybe aegerita extract. The method has a reasonable process and high extraction efficiency, is simple to operate and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting tea tree mushroom polysaccharide from tea tree mushroom, and the invention relates to the field of biomedicine. Background technique [0002] Tea Tree Mushroom, scientific name: agrocybeaegirit, was originally a wild fungus that grows on the part of the tea tree in the alpine dense forest area in Lichuan, Jiangxi. Tea tree mushroom contains 18 kinds of amino acids needed by the human body, especially 8 kinds of amino acids that cannot be synthesized by the human body, dextran, mycoprotein, carbohydrates and other nutrients. It is also rich in B vitamins and various mineral elements, such as iron, potassium, zinc, selenium and other elements, which are higher than other fungi. Traditional Chinese medicine believes that the mushroom has the functions of nourishing the kidney, diuresis, treating back pain, dampness, strengthening the spleen, Antidiarrheal and other effects, is an ideal food for patients wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07A23L33/105
Inventor 陈先锐
Owner GUANGXI NANNING SHENGQIAN TECH DEV CO LTD
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