Novel planar chromatography sample application instrument and step repeated sample application method

Abstract

The invention discloses a novel sample applicator for planar chromatography. The structure of the novel sample applicator comprises a temperature control sample application platform, an airflow control system and a motion control system. The applicator adopts a step-and-repeat sample application principle, a stationary phase absorbs a sample solution gradually in certain area range, and various modes are designed to promote solvent volatilization, so that a sample strip up to the mustard is formed. The working principle is as follows: gas output by a miniature air pump of the applicator or an externally connected air source enters a trace nozzle through adjustment of a gas measuring apparatus; the horizontally moving trace nozzle carries a trace sample application needle to perform uniform motion when a vertically moving pressing piece in the motion control system uniformly pushes a solution in the trace sample application needle out, the vertical movement and the horizontal movement are mutually matched, then the gas ejected by the trace nozzle carries the sample solution and is ejected to the surface of the stationary phase, and the uniform sample strip is formed. The sample applicator is stable in property, good in sample application repeatability and capable of meeting the sample application requirements for planar chromatographic analysis and separating preparation.

Description

technical field [0001] The invention is a novel planar chromatogram spotting instrument, which belongs to the field of planar chromatographic analysis instruments among analytical instruments. This instrument can be widely used in chemical, chemical, pharmaceutical, food, environmental protection, agriculture, forestry and other industries for qualitative and quantitative analysis and separation and preparation of a small amount of compounds using planar chromatography. Background technique [0002] Plane chromatography is an operational form of chromatographic techniques, including thin-layer chromatography and paper chromatography, of which thin-layer chromatography is the most commonly used. Thin-layer chromatography is a planar chromatography technique that coats a stationary phase (such as silica gel, polyamide, alumina, etc.) on a planar carrier (such as glass, aluminum foil, polyester plate, etc.) to separate samples. Qualitative identification, quantitative determ...

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Problems solved by technology

Due to the limitations of various factors, the units using imported samplers are not widely used

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