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Cyclamen tissue culture rapid propagation method

A technology of tissue culture rapid propagation and cyclamen, applied in the field of plant tissue culture, can solve the problems of slow reproduction of cyclamen, and achieve the effect of shortening the reproduction cycle and growing robustly.

Inactive Publication Date: 2015-10-21
韦键
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for rapid propagation of cyclamen tissue culture, which can solve the problem of slow propagation speed of cyclamen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Select budded flower stems of strong cyclamen plants, cut them into 1 cm segments, soak them in 75% alcohol for 30 seconds, and rinse them with sterile water for 5 times; inoculate the segments into bud induction medium, adjust the light to 2200LUX, adjust Temperature to 23°C, cultivate for 10 days until new shoots grow;

[0017] When the new sprout grows to 1 cm, it is inoculated in the strong seedling medium, and the light is adjusted to 2300 Lux, the temperature is 23 ° C, and it is cultivated for 18 days until the height of the seedling is 1.5 cm; the above-mentioned strong seedling is transferred into the rooting medium, and the light is adjusted. 2200 Lux, temperature is 23 ℃, cultivates for 20 days until the root of seedling grows 4 centimeters, obtains the cyclamen tissue culture seedling; The seedlings were transplanted to vermiculite, peat, sawdust in a seedling tray with a mass ratio of 1:2:2 for seedling cultivation, the air humidity was controlled to be 85%...

Embodiment 2

[0020] Select budded flower stems of strong cyclamen plants, cut them into small sections of 1.5 cm, soak them in 75% alcohol for 60 seconds, and rinse them with sterile water for 3 times; inoculate the small sections into bud induction medium, adjust the light to 2500LUX, adjust The temperature is 26°C, cultivated for 8 days until new shoots grow; when the sprouts grow to 2 cm, inoculate them in the strong seedling medium, adjust the light to 2500 Lux, and the temperature is 25°C, and cultivate for 13 days until the seedling height is 1 cm; Transfer the above-mentioned strong seedlings into the rooting medium, adjust the light to 2500 Lux, and the temperature is 26°C, and cultivate for 15 days until the seedling base grows 1 cm root to obtain the cyclamen tissue-cultured seedlings; When the roots grow to 1 cm, the cyclamen tissue culture seedlings are transplanted to vermiculite, peat, and the mass ratio of sawdust is 1:2:2 in the seedling tray for hardening and cultivating, a...

Embodiment 3

[0023] Select budded flower stems of robust cyclamen plants, cut them into 1.5 cm segments, soak them in 75% alcohol for 45 seconds, and rinse them with sterile water for 4 times; inoculate the segments into bud induction medium, adjust the light to 2400LUX, adjust Temperature to 25°C, cultivate for 10 days until new shoots grow; when the new shoots grow to 2 cm, inoculate them in the medium for strong seedlings, adjust the light to 2300 Lux, and cultivate at 25°C for 15 days until the height of the seedlings is 1 cm; Transfer the above-mentioned strong seedlings into the rooting medium, adjust the light to 2400 Lux, and the temperature is 25°C, and cultivate for 18 days until the seedling base grows 1 cm root to obtain the cyclamen tissue-cultured seedlings; When the roots grow to 1 cm, the cyclamen tissue culture seedlings are transplanted to vermiculite, peat, and the mass ratio of sawdust is 1:2:2 in the seedling tray for hardening and cultivating, and the air humidity is c...

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Abstract

The invention discloses a cyclamen tissue culture rapid propagation method and belongs to the technical field of plant tissue culture. The cyclamen tissue culture rapid propagation method comprises the specific steps that a flower stalk, with buds, of a cyclamen plant is selected and cut into small sections, disinfection is carried out, the small sections are inoculated to a bud induction culture medium, the illumination intensity and the temperature are regulated, culturing is carried out till new buds grow out, the small sections are inoculated again to a sound seedling culture medium, the illumination intensity and the temperature are regulated, culturing is carried out to obtain sound seedlings, the sound seedlings are inoculated again to a rooting culture medium, the illumination intensity and the temperature are regulated, culturing is carried out to obtain cyclamen tissue culture seedlings, the cyclamen tissue culture seedlings are transplanted into a seedling tray to carry out hardening seedling culture, the air humidity is controlled, the illumination intensity is regulated, hardening seedling culture is carried out, and the seedlings are finally transplanted to an open field to be cultured. By means of the cyclamen tissue culture rapid propagation method, the propagation period of cyclamen is shortened.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of cyclamen. Background technique [0002] Cyclamen persicum Mill., also known as radish crabapple, rabbit ear flower, rabbit flower, Yipinguan, bonfire flower, and petal lotus, is a perennial herb of Primulaceae and cyclamen. The leaves grow from the top of the tuber. Heart-shaped, ovate or kidney-shaped, the leaves have fine serrations, the leaf surface is green, with white or gray halo spots, the back of the leaf is green or dark red, the petiole is long, reddish brown. Cyclamen is a widely grown flower that is suitable for indoor pots and greenhouses in winter. Certain cultivars of cyclamen have a strong aroma, while others have little or no aroma. The word "cyclamen" comes from the transliteration of the scientific name Cyclamen. [0003] Cyclamen likes warmth and is afraid of heat. It grows best in a cool env...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 韦键
Owner 韦键
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