Method for evaluating calcification of biological valve in vitro and anti-calcification factor solution

Abstract

The present invention relates to the technical field of biological valves, and provides an in vitro biological valve calcification evaluation method, which includes the steps of preparation of calcification evaluation solution; RCI solution preheating; PRCI treatment; sample preservation; in vitro calcification reaction; calcium quantitative detection; The anti-calcification factor solution includes: alcohol reagent A, alkali solution B, grease and its derivatives reagent C and buffer solution reagent D; the evaluation method of the present invention has a short evaluation cycle, reduces evaluation procedures, and saves evaluation costs; at the same time The biological valve is chemically treated with the anti-calcification factor solution, some free chemical groups are blocked, and the binding force between the biological valve and calcium ions is reduced, thereby reducing the amount of calcium attached to the biological valve and enhancing the anti-calcification ability of the biological valve; Through the method for evaluating the calcification of the biological valve in vitro of the present invention, the anti-calcification performance of the chemically treated biological valve can be quickly and effectively judged.

Description

technical field [0001] The invention relates to the field of biological valves, in particular to an evaluation method for calcification of biological valves in vitro and an anti-calcification factor solution. Background technique [0002] At present, the incidence of aortic valve stenosis due to aortic valve calcification in the elderly over 65 years old is 2% to 7%, and the proportion of aortic valve stenosis increases with age. In patients with severe aortic stenosis, the left ventricular function is severely impaired, the quality of life of the patients is reduced, and the survival time is significantly shortened, so effective treatment is necessary. [0003] For patients with severe aortic valves with surgical indications, surgical artificial aortic valve replacement (whether mechanical valve or biological valve is used) is still the first choice of treatment, and the area of ​​the aortic valve after replacement is often greater than 1.5cm 2 . However, for those patien...

AI Technical Summary

Problems solved by technology

[0005] The method of evaluating biological valve calcification through animal clinical practice has certain limitations. It has high requirements for clinical surgery and clinicians' technical dependence, and has corresponding requirements for animal selection. At the same time, the breeding cycle is long and affected by the feeding environment and individual differences. Large, other symptoms may appear, such as bioprosthetic epidermis, growth vegetation, etc., which cannot accurately and effectively reflect the chemical interaction between bioprosthesis and calcium ions after chemical treatment

Existing methods not only increase the technical difficulty, but also greatly increase the time and cost. At the same time, the evaluation of the calcification performance of the biological valve is affected by various factors.

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