A kind of fermentation medium and its optimization method for improving spectinomycin production

Abstract

The present invention discloses a fermentation culture medium for improving the spectinomycin yield, and an optimization method thereof, and relates to the field of biopharmaceutics, wherein the fermentation culture medium is prepared from the following substances by weight: 1.0-3.0% of oral glucose, 3.0-6.0% of corn starch, 0.0-2.0% of fish powder, 0.5-2.0% of yeast powder, 0-2.0% of peanut cake powder, 0-2.0% of biological nitrogen, 0.05-0.25% of potassium dihydrogen phosphate, 0.01-0.2% of potassium chloride, 0.1-1.0% of ammonium sulfate, 0.01-0.05% of barbital, 0.1-1.0% of calcium carbonate, and the balance of water, wherein the pH value is 6.5-7.0. With the fermentation culture medium of the present invention, the spectinomycin production capability of the streptomyces adopted as the fermentation strain is substantially improved, the improvement effect of the spectinomycin yield is significant, and the fermentation culture medium is suitable for the large-scale stable and efficient fermentation production of spectinomycin.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a fermentation medium for increasing spectinomycin production and an optimization method thereof. Background technique [0002] Spectinomycin, the English name Spectinomycin, is a secondary metabolite produced by Streptomyces spectabilis after aerobic fermentation. It is an aminoglycoside antibiotic and has strong effects on both Gram-positive and Gram-negative bacteria It has high antibacterial activity against Neisseria gonorrhoeae, high sensitivity to penicillin and tetracycline-resistant Neisseria gonorrhoeae, and moderate antibacterial activity against many Enterobacteriaceae bacteria. Spectinomycin has the characteristics of broad bactericidal spectrum, no allergies, few side effects, and good safety. At present, it is clinically used to treat venereal diseases caused by Neisseria gonorrhoeae, and can also be used for animal treatment and can promote animal growth. It is ...

AI Technical Summary

Problems solved by technology

Patent CN103484509A proposes that the medium of spectinomycin seed tank and fermenter contains corn oil, maltose, brewer's yeast dry residue and earthworm powder, which solves the problem of cost and minimizes the source of raw and auxiliary materials without being affected by the environment. However, its raw materials are still subject to geographical restrictions, and the earthworm powder in its culture medium belongs to animal-derived raw materials. According to the GMP management regulations for veterinary and human use, it is difficult to achieve in industrial production.

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