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Manual germination accelerating method and seedling raising method of Bletilla sfriata (Thunb.)Reiehb.f.

A germination and artificial technology, applied in the field of agricultural science and technology, can solve the problems of high survival rate of seedling raising methods, short seedling raising time, and inability to wash solid medium, so as to improve seedling raising speed and efficiency, increase survival rate, and shorten seedling raising time Effect

Inactive Publication Date: 2015-12-02
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of this, the present invention provides an artificial germination method and a bletilla striata seedling raising method, the artificial germination method solves the problem that the solid medium carried by the roots of the seedlings cannot be cleaned, and the seedling raising method has a high survival rate and a short seedling raising time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The site is selected to complete this embodiment in Guanzhuang Lake Farm, Zhongxiang City, Hubei Province. The farm is located at about 30° north latitude, with an altitude of about 200m. The terrain is relatively flat, and a standardized controllable greenhouse of 3000m is built. 2 , 16 rows of seedbeds are laid inside, each row of seedbeds is 2m wide and 70m long, and the east-west and above of the greenhouse need to be shaded with 6-pin density sunshade nets, and the shading rate reaches 90-95%.

[0034] Bletilla striata seeds are artificially germinated in the laboratory, and the specific steps include:

[0035] (1) Storage and disinfection of Bletilla striata seeds: After harvesting Bletilla striata pods in October-November, seal them in plastic film bags and store them in a refrigerator at 4°C. This method can keep Bletilla striata seeds alive for 6-9 months. The disinfection process of Bletilla striata fruit pods should be completed on an ultra-clean workbench. F...

Embodiment 2

[0058] Embodiment 2 is basically the same as Embodiment 1, the difference is:

[0059] In this example, the seeds of Bletilla striata were artificially germinated in the laboratory, and the medium used was: 1 / 2MS medium was added with mashed potatoes, activated carbon, agar, NAA, 6-BA and locust bean gum to make mashed potatoes The concentration of activated carbon is 10g / L, the concentration of activated carbon is 2.0g / L, the mass percent of agar is 1.0%, the concentration of NAA is 1.2mg / L, the concentration of 6-BA is 1.0mg / L, the mass percent of locust bean gum 2.0%, pH 6.0.

[0060] In the seedling raising stage, the seedbed is prepared as follows: the ground is leveled, and then the matrix nutrient soil is spread on it, and the N, P, k, and organic matter contents of the matrix are respectively: 1.0g / kg, 0.5g / kg, 0.5g / kg, 70%, the width of the matrix compartment is 2m, the height is 15cm, and the length can be determined according to the plot. Before laying Bletilla st...

Embodiment 3

[0066] Embodiment 3 is basically the same as Embodiment 1, the difference is:

[0067] In this example, the seeds of Bletilla striata were artificially germinated in the laboratory, and the medium used was: 1 / 2MS medium was added with mashed potatoes, activated carbon, agar, NAA, 6-BA and locust bean gum to make mashed potatoes The concentration of activated carbon is 60g / L, the concentration of activated carbon is 1.2g / L, the mass percent of agar is 0.5%, the concentration of NAA is 1.1mg / L, the concentration of 6-BA is 0.8mg / L, the mass percent of locust bean gum 1.5%, pH 5.8.

[0068] In the stage of raising seedlings, the seedbed is prepared as follows: the ground is leveled, and then the matrix nutrient soil is spread on it, and the N, P, k, and organic matter contents of the matrix are respectively: 2.0g / kg, 2.0g / kg, 2.0g / kg, 40%, the width of the substrate compartment is 2m, the height is 15cm, and the length can be determined according to the plot. Before laying Bletil...

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Abstract

The invention provides a manual germination accelerating method. The method comprises the following steps: (1) inoculating seeds into a culture medium for cultivation, wherein the culture medium contains locust bean gum with mass percent being 0.1%-2.0%; and (2) inoculatingpaenibacillus xylanilyticusYUPP-1 onto the culture medium, and degrading the culture medium. The invention also provides application of the method in seedling raising of Bletilla sfriata (Thunb.)Reiehb.f. The application comprises the following steps: laying a substrate on the ground after performing germination accelerating by the method, spreading symbiotic strains of Bletilla sfriata (Thunb.)Reiehb.f. on a compartment surface to obtain a seedbed, and sowing buds of Bletilla sfriata (Thunb.)Reiehb.f. on the seedbed for cultivating and seedling raising. The manual germination accelerating method can be used for solving the problem that solid culture media carried on the roots of seedlings cannot be cleaned thoroughly, thereby enhancing the survival rate of seedlings remarkably; when the manual germination accelerating method is used by Bletilla sfriata (Thunb.)Reiehb.f. and is further applied to large-scale rapid seedling raising, the survival rate is improved to 95% or above, no dead seedling or rejuvenation period exists during transplanting to a field, and adult seedlings can be obtained by about 4-6 months in advance; the manual germination accelerating method is simple to operate and suitable for large-scale production, and has important guiding significance on manual large-scale plantation of orchidacea medicinal plants.

Description

technical field [0001] The invention belongs to the technical field of agricultural science and technology, and in particular relates to an artificial accelerating germination method and a bletilla striata seedling raising method. Background technique [0002] Bletilla striata, namely Bletillasfriata (Thunb.) Reiehb.f., is a terrestrial orchid plant, and its main medicinal part is the dried tuber of Bletilla striata, which mainly contains bletilla striata gum, and its content is as high as 50%. First of all, bletilla striata glue is non-toxic and non-irritating to the skin, and is a green and safe product; secondly, bletilla striata glue has the functions of stopping bleeding and nourishing the lungs, promoting muscle growth and relieving pain, etc. It is the main raw material of Yunnan Baiyao, Sunflower Weikangling, Band-Aids and other medicines; moreover, bletilla striata gum also has the effect of removing free radicals in the body and delaying skin aging, and is widely u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02A01G1/00
Inventor 周燚余磊磊孙正祥邓建新龚天芝方守国
Owner YANGTZE UNIVERSITY
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