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Amino acid sequence and application of a detection liver cancer marker imp1 antigenic epitope

An antigenic epitope and marker technology, applied in the biological field, can solve the problems of low sensitivity and poor specificity, and achieve the effect of high sensitivity and high specificity

Inactive Publication Date: 2017-08-29
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently reported autoantibody detection methods have low sensitivity and poor specificity, and the false negative rate can be as high as 50%.

Method used

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  • Amino acid sequence and application of a detection liver cancer marker imp1 antigenic epitope
  • Amino acid sequence and application of a detection liver cancer marker imp1 antigenic epitope
  • Amino acid sequence and application of a detection liver cancer marker imp1 antigenic epitope

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] kit preparation

[0027] 1 Reagents See Tab.2-7 for reagent preparation.

[0028]

[0029]

[0030]

[0031]

[0032]

[0033]

[0034] 2 operations

[0035] (1) Coating: The enzyme plate should be washed 3 times with washing buffer, the working antigen should be diluted to the working concentration with the coating solution, coated on the enzyme plate, and kept overnight at 4°C.

[0036] (2) Add glutamic acid: Wash 3 times with washing buffer, dilute glutamic acid with coating solution to a concentration of 100µg / ml, 200µl per well, incubate at 37°C or room temperature for 1h;

[0037](3) Add plasma and quality control control (primary antibody): Wash the ELISA plate 3 times with washing buffer, and dilute the plasma to an appropriate concentration with coating solution, generally 1:100-1:500, 100µl per well, Incubate at 37°C or room temperature for 1 hour;

[0038] (4) Secondary antibody incubation: wash 3 times with washing buffer, dilute the sec...

Embodiment 2

[0042] Detection of IMP1 auto-IgG antibody in patients with liver cancer

[0043] 1 Sample collection: In this study, 103 liver cancer samples confirmed by radiological examination and histological examination were selected from the Third Hospital of Jilin University and Provincial Cancer Hospital. All serum samples had not undergone any anti-cancer treatment before collection, and had comprehensive clinical data and information. At the same time, 121 healthy control samples were recruited. The possibility of liver cancer was ruled out by clinical interview and imaging examination. The healthy group and the liver cancer group were comparable in gender and age (P>0.05)

[0044] 2 Detection results: IMP1 autoantibody expression level (Tab.8): There was a statistical difference between the liver cancer group and the healthy control group ( Z =-5.167, P <0.001).

[0045] ROC curve analysis: the area under the ROC curve for IMP1 auto-IgG antibody detection in patients with l...

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Abstract

The epitope amino acid sequence for detecting liver cancer marker IMP1 and an application thereof belong to the technical field of immunology. The invention provides an epitope amino acid sequence of liver cancer related gene IMP1. An IMP1 polypeptide antigen is applied to detect corresponding specific autoantibodies in the blood of a patient with liver cancer, and the autoantibody can be used as a liver cancer marker to assess risks of liver cancer and prognostic effect. The antigenic polypeptide and antibody thereof can be used to prepare reagents for early diagnosis of liver cancer and prognosis and to develop a targeted drug for liver cancer treatment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a polypeptide fragment with detection liver cancer marker IMP1. Background technique [0002] Liver cancer is currently the second most common malignant tumor in my country in terms of morbidity and mortality. There are about 300,000 new cases of liver cancer every year, accounting for more than 50% of the world. Liver cancer develops rapidly and easily metastasizes, and is currently one of the most difficult malignant tumors with the worst prognosis. However, compared with other types of malignant tumors such as breast cancer, lung cancer, and prostate cancer, the molecular understanding of tumor markers related to the pathogenesis of liver cancer is still lacking. A large number of studies have shown that tumor-associated antigens in serum or plasma can induce the body to produce autoantibodies, and there are both tumor antigens and autoantibodies against the tumor ant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47G01N33/574
CPCC07K14/4748G01N33/57438
Inventor 孙世龙刘颖
Owner JILIN UNIV
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