Dissolved oxygen measuring method based on fluorescence quenching principle and application

A technology of fluorescence quenching and measurement method, used in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of inaccurate measurement results, low stability, interference of fluorescence light intensity, etc., to improve accuracy and stability, improve Accuracy, effect of removing bias in measurement results

Active Publication Date: 2015-12-09
OCEANOGRAPHIC INSTR RES INST SHANDONG ACAD OF SCI
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Problems solved by technology

Moreover, in the prior art, the measurement of dissolved oxygen concentration is calculated by measuring the intensity of fluorescence light to obtain the concentration of dissolved oxygen, but the intensity of fluorescence light is easily interfered by external light, resulting in inaccurate measurement results and low stability

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  • Dissolved oxygen measuring method based on fluorescence quenching principle and application
  • Dissolved oxygen measuring method based on fluorescence quenching principle and application
  • Dissolved oxygen measuring method based on fluorescence quenching principle and application

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Embodiment 1

[0036] figure 1 It is a schematic diagram of the principle of the dissolved oxygen measurement sensor based on the present invention, such as figure 1 As shown, the dissolved oxygen measuring instrument includes an excitation photodiode 1 , a reference photodiode 2 , an oxygen sensitive film 3 , a photodetector 4 , a photoelectric conversion board 5 and a communication control module 6 .

[0037] When measuring, first, place the sensor in the target sea area, control the excitation light diode 1 to emit blue-green light, and the blue-green light reaches the oxygen sensitive film 3, prompting the oxygen sensitive film 3 to react with dissolved oxygen in the water environment, and Red fluorescence is emitted, and the red fluorescence is transmitted to the photodetector 10, converted into an excitation photoelectric signal by the photoelectric conversion board 5 and transmitted to the communication control module 6, and then transmitted to the data terminal after communication pr...

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Abstract

The invention discloses a dissolved oxygen measuring method based on a fluorescence quenching principle and application, and belongs to the technical field of measurement of dissolved oxygen in a water body. The method includes the following steps of firstly, measuring the phase phi 1 of exciting light and the phase phi 2 of reference light to obtain the phase difference delta phi; secondly, calculating the oxygen partial pressure delta p through the phase difference delta phi obtained in the first step; thirdly, calculating the oxygen saturation degree S through the obtained delta p; fourthly, calculating the dissolved oxygen concentration [O2] through the oxygen saturation degree S obtained in the third step. By means of the method, the accuracy of the calculated dissolved oxygen concentration is high, and the result stability is high.

Description

technical field [0001] The invention relates to the technical field of dissolved oxygen measurement in water bodies, in particular to a method and application of dissolved oxygen measurement based on the principle of fluorescence quenching. Background technique [0002] The principle of fluorescence quenching, broadly speaking, includes any effect that can reduce the intensity of fluorescence. In a narrow sense, it only refers to the physical or chemical interaction process that occurs between fluorescent substance molecules and solvent or solute molecules, resulting in a decrease in fluorescence intensity. These substances that cause fluorescence quenching are called quenchers. [0003] The fluorescence quenching process is actually a process of competing with the luminescent process to shorten the lifetime of the excited state of the luminescent molecule. The fluorescence lifetime is a function of the phase of the received signal. The measurement mechanism of the existin...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 李正宝高皜宗芳伊贺海靖吴承璇刘杰吕斌祁国梁杨晓楠
Owner OCEANOGRAPHIC INSTR RES INST SHANDONG ACAD OF SCI
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