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Preparation method of composite probiotic preparation added into ruminant feed

A compound probiotic and ruminant technology, applied in animal feed, animal feed, microbial-based methods, etc., can solve the problems of low binding rate, high toxicity, pollution, etc., and achieve the effect of increased binding rate and enhanced absorption capacity

Inactive Publication Date: 2015-12-16
JIANGSU JINYU ENVIRONMENTAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem that the present invention mainly solves: for the current ruminant self directly absorbs and utilizes the ability of glucose very limited, and selenium is the trace element necessary for animals, but the current method of supplementing selenium is to add sodium selenite in the diet, resulting in combined In order to solve the problems of low production rate, high toxicity and serious pollution, a preparation method of compound probiotic preparation added to ruminant feed is provided. Cocci are used as strains for activation and cultivation. Glucose, etc. are used as raw materials, and sodium selenite is added to convert it into organic selenium through strain fermentation. Glycerin and other substances are produced during the fermentation process. Finally, corn flour is added as an adsorption carrier. After concentration, a compound probiotic preparation added to ruminant feed is obtained

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0026]First, the Candida krusei strain stored at 4°C was inoculated in solid activation medium, cultured at 30°C for 24 hours, and then transferred three times to restore the viability of the strain. After the activation was completed, the g Candida russii slant was inoculated in the seed medium, put it into a constant temperature shaker incubator, and cultured at 30°C at a speed of 100r / min for 24 hours to obtain seed liquid 1; In the solid activation medium, culture at 28°C for 30 hours, and then repeat the transfer 3 times, so that the vitality of the strain can be restored. The seed solution 2 was obtained by constant temperature cultivation for 18 hours; then the Streptococcus thermophilus strain was inoculated in the solid activation medium, cultured at 38°C for 36 hours, and then repeated transfer 3 times, so that the vitality of the strain could be restored. After the activation was completed, the produced Candida ruthenica slant was inoculated in the seed medium, and ...

example 2

[0029] Firstly, the Candida krusei strain stored at 5°C was inoculated in a solid activation medium, cultured at 35°C for 26 hours, and then transferred four times to restore the viability of the strain. After the activation was completed, the g Candida russii slant was inoculated in the seed medium, put it into a constant temperature shaker incubator, and cultured at 33°C at a speed of 130r / min for 26 hours to obtain seed solution 1; then the Candida russii strain was inoculated In the solid activation medium, culture at 29°C for 33h, and then repeat the transfer 4 times to restore the vigor of the strain. The seed liquid 2 was obtained by constant temperature cultivation for 22 hours; then the Streptococcus thermophilus strain was inoculated in the solid activation medium, cultured at 40°C for 42 hours, and then repeated transfer 4 times, so that the vitality of the strain was restored. After the activation was completed, the produced Candida ruthenica slant was inoculated i...

example 3

[0032] Firstly, the Candida krusei strain stored at 6°C was inoculated in a solid activation medium, cultured at 40°C for 30 hours, and then transferred 5 times to restore the viability of the strain. After the activation was completed, the g Candida russii slant was inoculated in the seed medium, placed in a constant temperature shaker incubator, and incubated at 35°C at a speed of 150r / min for 30 hours to obtain seed liquid 1; In the solid activation medium, culture at 30°C for 36h, and then repeat the transfer 5 times, so that the vigor of the strain can be restored. The seed liquid 2 was obtained by constant temperature cultivation for 24 hours; then the Streptococcus thermophilus strain was inoculated in the solid activation medium, cultured at 42°C for 48 hours, and then repeated transfer 5 times, so that the vitality of the strain was restored. After the activation was completed, the produced Candida ruthenica slant was inoculated in the seed medium, and cultured at 40°...

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Abstract

The invention discloses a preparation method of a composite probiotic preparation added into ruminant feed, belonging to the technical field of animal feed processing. The preparation method comprises the following steps: carrying out activation culture on Candida krusei, Candida utilis and Streptococcus thermophilus used as strains, carrying out strain fermentation by using glucose and the like as raw materials and adding sodium selenite to convert the sodium selenite into organic selenium, wherein glycerol and other substances are generated in the fermentation process; and finally, adding corn flour as an adsorption carrier, and concentrating to obtain the composite probiotic preparation added into ruminant feed. The example proves that the probiotic prepared by the method has the advantages of high combination rate with rumination feed and no toxicity or harm, contains abundant glycerol and organic selenium, and enhances the absorptivity of the ruminant.

Description

technical field [0001] The invention discloses a method for preparing a compound probiotic preparation added to ruminant feed, and belongs to the technical field of animal feed processing. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. Beneficial bacteria or fungi in humans and animals mainly include Clostridium butyricum, lactic acid bacteria, bifidobacteria, Lactobacillus acidophilus, actinomycetes, and yeasts. At present, the most powerful products researched in the world are mainly compound active probiotics composed of the above-mentioned various microorganisms, which are widely used in the fields of bioengineering, industry and agriculture, food safety, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23K1/00C12R1/72C12R1/46
Inventor 李锦宇林大伟张帆
Owner JIANGSU JINYU ENVIRONMENTAL ENG
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