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Laminaria brown seaweed introduction method based on gametophyte cloning line

A gametophyte and kelp technology, which is applied in the field of introduction of kelp brown algae based on gametophyte cloning lines, can solve the problems of large size of attached seedlings, unseparated male and female gametophytes, and low size, and achieves small occupied volume, improved success rate, and generation volume. small effect

Inactive Publication Date: 2015-12-23
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

This method can not only solve the problems of excessive sporophyte volume, easy to rot, loose spores and low quality during the introduction of large-scale economic brown algae, but also solve the problem of unseparated male and female gametophytes and large size of seedlings in the introduction of kelp in the past. And other issues

Method used

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  • Laminaria brown seaweed introduction method based on gametophyte cloning line
  • Laminaria brown seaweed introduction method based on gametophyte cloning line
  • Laminaria brown seaweed introduction method based on gametophyte cloning line

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Embodiment 1

[0028] This embodiment takes the introduction of extreme northern kelp as an example, and the specific steps include:

[0029] 1. Algae selection and pretreatment. Select the sea area where the target seaweed grows luxuriantly. When the tide ebbs to the lowest line, select algae from the seaweed population. The criteria for selecting algae are as follows: the individual algae are large and complete, the color is normal and shiny, there are no other attached organisms on the surface, spores The bladder is full. Use a sterile scalpel to cut the sporophyte tissue block with sporangia, put it in an ice box, control the temperature at about 4°C, and bring it back to the laboratory as soon as possible.

[0030] The sporophyte tissue pieces brought back to the laboratory were cleaned with sterilized seawater, and the surface of the algae was gently wiped with sterilized degreasing cotton to remove attachments; the cleaned sporophytes were placed in a cool and ventilated place (tempe...

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Abstract

The invention provides a laminaria large-scale economical brown seaweed introduction method based on a gametophyte cloning line, belonging to the technical field of marine organisms. The laminaria large-scale economical brown seaweed introduction method comprises the steps of selecting and pre-treating parent seaweeds, collecting spores, culturing gametophytes, separating female and male gametophytes, transporting the gametophyte cloning line in a long distance, propagating the gametophyte cloning line and preserving the gametophyte cloning line for a long period. According to the laminaria large-scale economical brown seaweed introduction method, by using the characteristics that laminaria seaweed gametophytes have small sizes in all generations and are easy to preserve, the laminaria seaweed gametophytes occupy a small space when being transported in the long distance, and a culture solution does not need to be replaced; and the laminaria large-scale economical brown seaweed introduction method is simple and feasible and the success rate can be up to 100%.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a method for introducing brown algae of the genus Laminaria based on gametophytic cloning lines. Background technique [0002] Seaweed is an important marine resource and has a wide range of uses. It can be used as food. For example, kelp and seaweed are popular marine foods; seaweed is also the bait for marine animals such as abalone and sea cucumber, and it is also used to extract iodine, alginate and manna Alcohol is an important chemical raw material. In addition, seaweed is also of great significance to the restoration and protection of the offshore ecological environment. Seaweed breeding is an important way for efficient and sustainable utilization of seaweed resources. After decades of development, seaweed farming has become an important part of my country's marine industry. Taking brown algae kelp as an example, the annual breeding area is about 37,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘福利王飞久孙修涛汪文俊梁洲瑞
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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