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Molecular-specific marker primers and methods for identifying Houttuynia cordata and Baibu Huanhun

A technology of labeling primers and Houttuynia cordata, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., can solve problems such as difficult to judge whether it is mixed, misleading, and difficult

Active Publication Date: 2018-04-13
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, once dried or processed, it is difficult to accurately identify them using these traditional methods
However, it is easy to be misled by the analysis of chemical components only based on specific chemical components, and it is difficult to judge whether it is mixed.

Method used

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  • Molecular-specific marker primers and methods for identifying Houttuynia cordata and Baibu Huanhun
  • Molecular-specific marker primers and methods for identifying Houttuynia cordata and Baibu Huanhun

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Experimental program
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Effect test

Embodiment 1

[0012] 1 instrument

[0013] PCR instrument (Eppendorf, model 5332), electrophoresis system (Beijing Liuyi Instrument Factory, model DYY-12), low-temperature refrigerated centrifuge (Eppendorf, model 5810R), gel imaging analyzer (BIO-RAD ChemiDoc XRS), trace Pipettes (Eppendorf).

[0014] 2 reagents

[0015] 2×CTAB extract, 1×TAE buffer, agarose (Promega Company), ethidium bromide (Fluka Company), TransStart®TopTaq DNA Polymerase (Beijing Quanshijin Company), chloroform, absolute ethanol, iso All propanol is of domestic analytical grade.

[0016] 3 materials

[0017] The experimental samples in this study were collected from different origins and identified as Houttuynia cordata Thunb and Gymnotheca chinensis Decne.

[0018] 4 methods

[0019] DNA extraction:

[0020] ⑴ Take 5 g of fresh Houttuynia cordata and the leaves of Baibu Huansheng plant, cut them into pieces, put them in a sterilized mortar, add 0.5g of PVP powder, then add liquid nitrogen to grind quickly, coll...

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Abstract

The invention relates to a molecular-specific marker primer and a method for distinguishing Houttuynia cordata and Houttuynia cordata, belonging to the field of biotechnology. The primers are: the upstream specific primer HtF that specifically recognizes Houttuynia cordata: 5'-TTTTCTTCGTCATTGATTCC-3', the upstream specific primer GcF that specifically recognizes Houttuynia: 5'-AGGAAGAAGCGAATATTCCG-3', and the downstream universal primer trnL‑Fr: 5′‑TCCTC TGCTCTACCAGCTGAG‑3′. This method is based on the molecular identification of the genetic background and is not affected by external factors such as the place of origin of the medicinal material. Compared with the chemical method, it is more accurate, simpler and more convenient.

Description

technical field [0001] The invention relates to a molecular specific marker primer for distinguishing Houttuynia cordata Thunb and Gymnotheca chinensis Decne, and a method for rapidly identifying Houttuynia cordata Thunb and Gymnotheca chinensis Decne by using the specific primer The method belongs to the field of biotechnology. Background technique [0002] The traditional Chinese medicine Houttuynia cordata is a plant of the family Sanbaiaceae (Houttuynia cordata) ( Houttuynia cordata Thunb ) fresh whole grass or dry aerial parts. The traditional Chinese medicine Baibu Huansheng is a plant of the same family as the naked capsule ( Gymnotheca chinensis Decne ) whole grass or leaves. The functions and indications of the two are different, but because of the same distribution, often mixed, and similar shapes, it is easy to be misused and misused; they are common Houttuynia cordata and should be strictly distinguished. The identification of Houttuynia cordata and these co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6895
Inventor 陈莹闫淑君邓传远
Owner FUJIAN AGRI & FORESTRY UNIV
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