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A new method for rapid discovery of low-level active ingredients in natural products

An active ingredient, low-content technology, which is applied in the field of compound extraction and separation in natural products, can solve the problem of inability to effectively solve the problem of finding low-content active ingredients, has not yet reached the effective concentration, and is difficult to find, etc., and achieves low cost and automation. High-level, easy-to-use effects

Active Publication Date: 2018-03-09
CHINA PHARM UNIV
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  • Claims
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Problems solved by technology

However, because this method does not treat the high and low content components in complex natural products differently, in the process of fraction preparation based on the full component chromatographic fingerprint, when the preparation amount of the high content components reaches the effective concentration of activity screening, the low Due to the low preparation amount, the content components are often difficult to be found before reaching the effective concentration
Therefore, this method cannot effectively solve the problem of finding low-level active ingredients.

Method used

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  • A new method for rapid discovery of low-level active ingredients in natural products
  • A new method for rapid discovery of low-level active ingredients in natural products
  • A new method for rapid discovery of low-level active ingredients in natural products

Examples

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Embodiment 1

[0054] Example 1: Rapid Screening of Trace Active Components Activating Nrf2-ARE Pathway in Salvia Miltiorrhiza Extract

[0055] 1. Instruments and materials

[0056] The high-performance liquid phase system is Agilent 1100HPLC system (Agilent, Germany); quadrupole mass spectrometer detector (Agilent, Germany), with electrospray ion source (ESI), double high-pressure pump, degasser, disc autosampler, Column oven and DAD detector; Sovall Evolution RC high-speed refrigerated centrifuge (KENDRO, USA); rotary evaporator (Büchi, Switzerland); LABCONCO freeze-drying apparatus; BP 211D electronic balance per hundred thousandth (Sartorius, Germany) ; Solvent Evaporation Workstation (Gene Vac EZ-2).

[0057] Ultra-clean table (Thermo Company of the United States, Thermo Scientific 1300A2 type); CO 2 Incubator (Thermo Company, USA, series 2Water Jacket); vertical ultra-low temperature preservation box (Haier Company, DW-86L386); centrifuge (Thermo Company, USA, Fresco17); microplate r...

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Abstract

The invention relates to the field of natural medicines, in particular to a method for quickly discovering low-content active ingredients in natural products. The method divides the whole components of natural medicine into high-content components and low-content components through relative quantification, and uses the high-resolution chromatographic peak fraction preparation method to directly establish a high-content fraction library and obtain corresponding mass spectrum information. After knocking out these high-content components, the low-content components were enriched, and the low-content fraction library was obtained after solvent concentration and secondary separation, and the mass spectrum information of the low-content components was obtained at the same time. On this basis, activity screening was carried out on high and low content fractions respectively. This method has the advantages of rapidity, precision, high degree of automation, simple operation, low cost, and strong applicability, and is suitable for the discovery of low-level active ingredients in natural products.

Description

technical field [0001] The invention relates to the field of compound extraction and separation in natural products, in particular to a method for quickly discovering low-content active ingredients in complex natural products. technical background [0002] Natural medicines mainly refer to single-component or multi-component medicines with clear therapeutic effects of natural sources of plants (herbal medicine), animals, microorganisms, marine organisms and other organisms. [0003] Natural medicines are an important source of modern innovative drug discovery. Many compounds from natural sources have been found to have diverse and unique physiological activities, and a large number of drugs with special therapeutic effects have been developed on this basis. Natural active substances often have the characteristics of novel structure, high activity, and few adverse reactions. They are an important source of new drug research and development in the pharmaceutical industry, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/34
Inventor 陈君李雪岩杨琳李萍张柳
Owner CHINA PHARM UNIV
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