Applications of hepatocyte growth factor-regulated tyropsine kinasesubstrate in preparation of medicines preventing enterovirus 71-type infection

A technology of hepatocyte growth factor and tyrosine kinase, applied in the field of biomedicine

Active Publication Date: 2016-01-27
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there are no reports on the role of VPS4A and HRS molecules in EV71-infected host cells (especially Caco-2 cells). In-depth research on these two molecules

Method used

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  • Applications of hepatocyte growth factor-regulated tyropsine kinasesubstrate in preparation of medicines preventing enterovirus 71-type infection
  • Applications of hepatocyte growth factor-regulated tyropsine kinasesubstrate in preparation of medicines preventing enterovirus 71-type infection
  • Applications of hepatocyte growth factor-regulated tyropsine kinasesubstrate in preparation of medicines preventing enterovirus 71-type infection

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Experimental program
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Embodiment 1

[0049] 1. Design and synthesize specific siRNA sequences for each host cell molecule.

[0050] 1.1 For each target gene, search NCBIGeneBank to obtain the full sequence and mRNA sequence, use existing network resources and common software to conduct biological analysis on each target gene, and select the coding region as the target sequence for siRNA design. Refer to the siRNA design principles, and compare the blast function of the GeneBank database with the human genome sequence to ensure that there is no homology; exclude potential siRNAs that have 8 consecutive bases at the 5'end of the aitisense chain paired with other genes; exclude any segment of consecutive 14 Potential siRNA paired with one base to other genes. The design software was used for pre-evaluation and determination, and the 3 best kinetic parameter targets were selected to enter the subsequent experimental process. A total of 3 interference sequences were synthesized for each gene, as shown in Table 1.

[0051]...

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Abstract

The invention relates to the biomedical technology field, and provides a new target spot for resisting enterovirus 71-type infection and applications. Human colon cancer cells (Caco-2) are employed as target cells, the RNA interference technology is employed to reduce expression of the target cell host proteins, a host factor capable of inhibiting EV71 infection of human colon cancer cells (Caco-2) effectively is sought and the purpose of cutting off EV71 infection from the source (intestinal tract) effectively is achieved. Experiments show that hepatocyte growth factor-regulated tyropsine kinasesubstrate (HRS) plays an important role in inhibiting EV71 infection of Caco-2, the HRS expression is reduced, and EV71 infection can be inhibited obviously. Applications of HRS in preparation of medicines preventing or treating enterovirus 71-type infection are provided.

Description

Technical field [0001] The invention relates to the field of biomedical technology, and is a new target and application for resisting enterovirus 71 infection. Background technique [0002] Enterovirus 71 (enterovirus71, EV71) belongs to the picornaviridae Enterovirus is a human enteric A virus, and is one of the main pathogens that cause hand-foot-mouth disease (HFMD). At present, hand-foot-mouth disease is outbreak and endemic in many parts of the world, especially in the Asia-Pacific region. In my country, since the outbreak of hand-foot-mouth disease in several major provinces and cities in 2008, the number of infections and mortality of the disease have been high, with more than 1 million cases and nearly 1,000 deaths reported each year. The main morbidity of hand, foot and mouth disease is infants and young children under 5 years of age. The clinical manifestations are fever, herpes and other symptoms in the hands, feet, buttocks, and oral mucosa; a small number of childre...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P31/14
Inventor 朱勇喆任浩戚中田陈生林赵平徐庆强
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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