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Application of heme oxygenase-2 in preparing preparations inhibiting organ transplantation immune rejection

A technology of heme oxidase and immune rejection is applied in the field of preparing preparations for inhibiting immune rejection in organ transplantation, which can solve the problems of reducing immune rejection and low long-term survival rate, and achieve the effects of improving survival rate and inhibiting immune rejection.

Inactive Publication Date: 2016-02-03
XUZHOU MEDICAL COLLEGE
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AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of low long-term survival rate of transplanted organs due to immune rejection, we prepared HO-2 transgenic mice to study the effect of HO-2 on reducing immune rejection in mouse skin allografts, and then provided Application of HO-2 and its gene in preparation of preparation for suppressing immune rejection of organ transplantation

Method used

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  • Application of heme oxygenase-2 in preparing preparations inhibiting organ transplantation immune rejection
  • Application of heme oxygenase-2 in preparing preparations inhibiting organ transplantation immune rejection
  • Application of heme oxygenase-2 in preparing preparations inhibiting organ transplantation immune rejection

Examples

Experimental program
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Effect test

Embodiment 1

[0011] Example 1: Amplification of HO-2 gene and construction of eukaryotic expression vector pEF1α-HO-2-AcGFP

[0012] Total RNA was extracted from the hippocampus of C57BL / 6 mice, and cDNA was synthesized using TaKaRa reverse transcription kit. Use cDNA as template. Using F:5'-GTGAATTCATGTCTTCAGAGGTGGAGACC(EcoRI)-3'(SEQIDNO.1), R:5'-TAGGATCCCATGTAGTACCAGGCCAATAG(BamHI)-3'(SEQIDNO.2) as upstream and downstream primers to amplify the 848bp fragment of HO-2. Incremental conditions: 94°C for 4 min, 94°C for 40s, 60°C for 40s, 72°C for 40s, 72°C for 10min, 30 cycles. The amplification product was electrophoresed on 1% agarose gel to determine the size of the amplification product, and then the PCR product of H0-2 was recovered and purified, and the vector pEF1α-IRES-AcGFP (purchased from Clontech) was simultaneously treated with EcoRI and BamHI double enzymes After digestion, 1% agarose gel electrophoresis was carried out, and the products of both digestions were recovered by a...

Embodiment 2

[0013] Example 2: Establishment of HO-2 Transgenic Animal Model

[0014] Transgenic mice of HO-2 were prepared by microinjection technology (assisted by Guangzhou Saiye Biological Company).

Embodiment 3

[0015] Example 3: Mouse skin transplantation experiment

[0016] The skins of adult HO-2 transgenic FVB mice and wild-type FVB mice were transplanted to the backs of adult BALB / c mice, respectively, and 10 positive mice and wild-type mice were selected for each time period to observe the skin survival rate. figure 1 As shown, the HO-2 transgenic (Tg) transplanted skin was compared with the wild type (W) on the 2nd day (A1, A2), and the survival rate of the A1 skin was not significantly different from that of the A2 skin; on the 4th day, the B1 skin was more than B2 skin, The survival rate of B1 skin was 70% and that of B2 skin was 40%, and the difference in survival rate between the two reached a significant level; on the 8th day, the survival rate of C1 skin was 50% compared with C22 skin, and the survival rate of B2 skin was 20%. The difference in survival rate between the two reached a significant level. The 2d, 4d, and 8dd positive skin and negative skin were subjected to...

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Abstract

The invention relates to application of heme oxygenase-2 in preparing preparations inhibiting organ transplantation immune rejection, and discloses application of heme oxygenase-2 and genes thereof in preparing preparations inhibiting organ transplantation immune rejection. The preparation can be used for inhibiting organ transplantation immune rejection and can increase the organ transplantation survival rate, and an effective method is provided for treating chronic end-stage diseases of the liver, the kidneys, the heart and the like.

Description

technical field [0001] The invention relates to the application of heme oxidase-2 in the preparation of preparations for inhibiting immune rejection of organ transplantation. Background technique [0002] Organ transplantation is the main method for the treatment of chronic end-stage diseases such as liver, kidney and heart, and suppression of rejection is one of the important factors for the long-term survival of transplanted organs. With the progress of research, the role of the self-protective response of transplanted organs in preventing graft rejection has gradually attracted attention. The protective effect is mainly mediated by protective genes. Through research, it is found that the expression of protective genes is in the smooth muscle cells and vascular endothelial cells of the transplanted surviving organs, and there is little or no expression in the transplanted organs with rejection. Heme Oxygenase-2 (HO-2) HO-2 is an important endogenous anti-apoptotic factor,...

Claims

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Application Information

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IPC IPC(8): A61K38/44A61K48/00A61P37/06
Inventor 陈仁金王珍珍陈全刚袁红花吴连连
Owner XUZHOU MEDICAL COLLEGE
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