Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cell cryopreservation liquid capable of enabling cells to survive for long time, cell preparation and preparation method

A technology for cryoprotective solution and cell preparation, applied in the field of medicine, can solve the problems of decreased cell viability, consuming manpower and material costs, affecting the effect of reinfusion treatment, etc., and achieves the effect of ensuring effectiveness and reducing production costs.

Inactive Publication Date: 2016-02-24
北京佳宸弘生物技术有限公司
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the cells of this form of cell preparation are relatively "fresh", there are also significant limitations
For example, it is best to use the cell preparation within 4 hours after preparation, otherwise the cell viability will drop sharply, which will affect the effect of reinfusion therapy; at the same time, the departments carrying out cell therapy must invest huge sums of money in the establishment of corresponding cell culture GMP laboratories (The premise must be that there is a corresponding site for the construction of the laboratory), and then recruit relevant technical personnel to prepare cell preparations nearby, which not only consumes a lot of manpower and material resources, but also greatly limits the use of cell preparations: only in The preparation should be used as soon as possible within 4 hours after the preparation is completed, and it cannot be provided to external units that need it and have a long journey

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0084] The cryoprotective solution for cells, cell preparations and preparation methods of the present invention that can enable cells to survive for a long time will be further described in detail below through specific embodiments.

[0085] 1. This embodiment provides a cell cryoprotectant solution that can make cells survive for a long time. It is a clinical-grade cell cryoprotectant solution. Cell preparations prepared with this cryoprotectant solution mainly include three categories: ① Stem cell preparation mainly contains: 80% (v / v) human serum albumin + 20% (v / v) CryoSure-DEX40 freezing solution + 1×10 7 -6×10 7Mesenchymal stem cells, the final volume of the preparation is 8ml. ②Immune cell preparations mainly include: 80% (v / v) human serum albumin + 20% (v / v) CryoSure-DEX40 freezing solution + 1×10 9 -2×10 9 Personal peripheral blood / umbilical cord blood-derived cytokine-induced killer cells (CIK), the final volume of the preparation is 20ml. ③Immune cell preparati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses cell cryopreservation liquid capable of enabling cells to survive for long time, a cell preparation and a preparation method. The cell cryopreservation liquid is mixed liquid containing human serum albumins and CryoSure-DEX40 cryopreservation liquid. The cell preparation comprises the cell cryopreservation liquid provided by the invention and cells. The preparation method comprises the following steps: uniformly mixing the CryoSure-DEX40 cryopreservation liquid and the human serum albumins to form the cell cryopreservation liquid capable of enabling the cells to survive for long time and the cell cryopreservation liquid is slowly injected into washed cell sediment; and the cell cryopreservation liquid is injected and then is uniformly mixed to finally form the cell preparation. After the cell preparation prepared by the preparation method provided by the invention is cryopreserved for long time or transported for a long distance, the survival rate of the cells is still very high; and the cell cryopreservation liquid has a clinic-grade application value; and the limitation that a previous cell preparation can be transported while being prepared is broken through and a utilization geographical range of the cell preparation is enlarged.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a cell cryoprotectant solution capable of keeping cells alive for a long time, a cell preparation and a preparation method. Background technique [0002] The 21st century is the era of cell therapy. The current cell therapy is mainly divided into two categories: immune cell therapy and stem cell therapy. The current model adopted in the field of cell therapy is generally to build a laboratory near the hospital to prepare the cell preparation nearby. After the cell preparation is prepared, it is best to infuse it within 4 hours. Such a cell preparation is mainly composed of: 90% (v / v) 0.9% normal saline + 10% (v / v) human serum albumin (the concentration is not strictly limited) + a certain number of cells (stem cells or immune cells) , the cells were in a non-frozen state. In view of the fact that the cells are directly transferred from the culture medium to the solvent mainly composed ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 魏俊宁韩莹杨轶
Owner 北京佳宸弘生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products