Cell cryopreservation liquid capable of enabling cells to survive for long time, cell preparation and preparation method
A technology for cryoprotective solution and cell preparation, applied in the field of medicine, can solve the problems of decreased cell viability, consuming manpower and material costs, affecting the effect of reinfusion treatment, etc., and achieves the effect of ensuring effectiveness and reducing production costs.
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[0084] The cryoprotective solution for cells, cell preparations and preparation methods of the present invention that can enable cells to survive for a long time will be further described in detail below through specific embodiments.
[0085] 1. This embodiment provides a cell cryoprotectant solution that can make cells survive for a long time. It is a clinical-grade cell cryoprotectant solution. Cell preparations prepared with this cryoprotectant solution mainly include three categories: ① Stem cell preparation mainly contains: 80% (v / v) human serum albumin + 20% (v / v) CryoSure-DEX40 freezing solution + 1×10 7 -6×10 7Mesenchymal stem cells, the final volume of the preparation is 8ml. ②Immune cell preparations mainly include: 80% (v / v) human serum albumin + 20% (v / v) CryoSure-DEX40 freezing solution + 1×10 9 -2×10 9 Personal peripheral blood / umbilical cord blood-derived cytokine-induced killer cells (CIK), the final volume of the preparation is 20ml. ③Immune cell preparati...
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