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Heat shock protein 90 (HSP90) chemiluminescence detection kit

A technology of chemiluminescence detection and heat shock protein, which is applied in the field of clinical blood immune detection, can solve the problems of many influencing factors, false positive clinical requirements, narrow detection range, etc., and achieve the effect of high sensitivity and strong specificity

Inactive Publication Date: 2016-03-16
BEIJING DIAGREAT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the technology is mature, advanced and practical. The methods widely used in the quantitative analysis of PAP are mainly radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA). Among them, RIA must be labeled with radioactive elements such as 125I. The detection equipment is complicated and expensive, and special instruments must be used for measurement. The half-life of the radionuclide is short, and it cannot be stored for a long time. The measurement results are unstable, and it also brings radiation damage to the operator
ELISA detection sensitivity is not high enough, the detection range is narrow, there are many influencing factors, it is easy to cause false negatives and false positives, and it cannot meet the clinical requirements.

Method used

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  • Heat shock protein 90 (HSP90) chemiluminescence detection kit
  • Heat shock protein 90 (HSP90) chemiluminescence detection kit
  • Heat shock protein 90 (HSP90) chemiluminescence detection kit

Examples

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Effect test

Embodiment 1

[0044] A heat shock protein 90 (HSP90) chemiluminescent detection kit, comprising the following components:

[0045] ① heat shock protein 90 (HSP90)) standard product, the heat shock protein 90 referred to as HSP90;

[0046] ②Magnetic particles coated with heat shock protein 90 (HSP90) monoclonal antibody;

[0047] ③ Enzyme-labeled heat shock protein 90 (HSP90) monoclonal antibody;

[0048] ④ Chemiluminescent substrates for the action of the above enzymes;

[0049] ⑤ washing liquid;

[0050] ⑥ White opaque microwell plate.

[0051] Wherein, the magnetic particles coated with HSP90 monoclonal antibody are prepared through the following steps:

[0052] ① Take 5.0ml of magnetic particle suspension with a mass content of 2.5%, magnetic plate separation, discard the supernatant, and wash the magnetic particles with deionized water; the liquid in the magnetic particle suspension has a concentration of 0.05mol / L and a pH value of Phosphate buffer saline of 7.4;

[0053] ②Wash t...

Embodiment 2

[0077] A heat shock protein 90 (HSP90) chemiluminescent detection kit, comprising the following components:

[0078] ① heat shock protein 90 (HSP90)) standard product, the heat shock protein 90 referred to as HSP90;

[0079] ②Magnetic particles coated with heat shock protein 90 (HSP90) monoclonal antibody;

[0080] ③ Enzyme-labeled heat shock protein 90 (HSP90) monoclonal antibody;

[0081] ④ Chemiluminescent substrates A and B for the above-mentioned enzyme action;

[0082] ⑤ washing liquid;

[0083] ⑥ White opaque microwell plate.

[0084] Wherein, the magnetic particles coated with HSP90 monoclonal antibody are prepared through the following steps:

[0085] ① Take 5.0ml of magnetic particle suspension with a magnetic particle mass content of 2.5%, magnetic plate separation, discard the supernatant, and wash the magnetic particles once with deionized water; the liquid in the magnetic particle suspension has a concentration of 0.05mol / L, Phosphate buffer with a pH value ...

Embodiment 3

[0109] A heat shock protein 90 (HSP90) chemiluminescent detection kit, comprising the following components:

[0110] ① heat shock protein 90 (HSP90)) standard product, the heat shock protein 90 referred to as HSP90;

[0111] ②Magnetic particles coated with heat shock protein 90 (HSP90) monoclonal antibody;

[0112] ③ Enzyme-labeled heat shock protein 90 (HSP90) monoclonal antibody;

[0113] ④ Chemiluminescent substrates A and B for the above-mentioned enzyme action;

[0114] ⑤ washing liquid;

[0115] ⑥ White opaque microwell plate.

[0116] Wherein, the magnetic particles coated with HSP90 monoclonal antibody are prepared through the following steps:

[0117] ① Take 5.0ml of magnetic particle suspension with a magnetic particle mass content of 2.5%, magnetic plate separation, discard the supernatant, and wash the magnetic particles once with deionized water; the liquid in the magnetic particle suspension has a concentration of 0.05mol / L, Phosphate buffer with a pH value ...

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Abstract

The present invention provides a heat shock protein 90 (HSP90) chemiluminescence detection kit, which comprises: 1) a heat shock protein 90 (HSP90)) standard substance, wherein the heat shock protein 90 is short for HSP90; 2) magnetic particles coated with a HSP90 monoclonal antibody; 3) an enzyme-labeled HSP90 monoclonal antibody; 4) chemiluminescent substrates A and B acted by the enzyme; 5) a washing solution; and 6) a white opaque micro-pore plate. According to the present invention, with the kit, the HSP90 content in patient serum can be detected, and the important guide significance is provided for detection of non-small cell lung cancers and other tumors; and the kit has characteristics of high sensitivity, strong specificity, safety, and reliability.

Description

technical field [0001] The invention belongs to the technical field of clinical blood immune detection. Specifically, the invention provides a heat shock protein 90 (HSP90) chemiluminescent detection kit and a preparation method thereof. The kit of the invention has the advantages of simplicity, speed, sensitivity, stability and the like. The detection kit has high sensitivity and strong specificity. Background technique [0002] Shock protein (HeatShockProtein, HSP) was discovered by geneticist Ritossa in 1962, which is a kind of protein that is highly conserved in the process of biological evolution and widely exists in prokaryotes and eukaryotes. Heat shock protein is a protein synthesized by organisms in response to physical, chemical, biological, and mental stimuli in the environment. It can maintain the function of signal transduction proteins and is an important buffer factor in the process of cell mutation. It is responsible for the spatial structure and mutation o...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N21/76
CPCG01N33/6872G01N21/76G01N33/57423
Inventor 许秀丽
Owner BEIJING DIAGREAT BIOTECH CO LTD
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