Novel multifunctional airlift tube photobioreactor

A technology of photobioreactor and reaction device, which is applied in the direction of specific-purpose bioreactor/fermenter, combination of bioreactor/fermenter, microorganisms, etc., which can solve difficulties, increase the pollution of harmful organisms, and cut algae cells Harm and other problems, to enhance the absorption and analysis of oxygen, to avoid shear damage, to avoid the effect of oxygen stress

CN105462807AInactive Publication Date: 2016-04-06JIAXING ZEYUAN BIOLOGICAL PROD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2016-04-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a reactor body and a multifunctional airlift tube photobioreactor. The reactor body, namely a reaction device, comprises two or more transparent perpendicular pipelines arranged side by side, and connecting tubes or connecting grooves communicated with the tops and the bottoms of the perpendicular pipelines respectively. The photobioreactor comprises the reactor body, a ventilation system and an optional parameter detection and control system. The invention further provides a microalgae culture method which comprises the step that the reactor body or the photobioreactor is used for carrying out photoautotrophic culture, photoinduction culture and mixotrophic nutrition culture of the microalgae. The multifunctional airlift tube photobioreactor has the advantages of being large in illumination specific surface area, high in gas-liquid mass transfer coefficient, small in fluid shear force and the like; engineering enlargement, cleaning and maintenance are easy, operation is convenient, high-density, high-efficiency and large-scale photoautotrophic culture, photoinduction culture and mixotrophic nutrition culture of the microalgae can be achieved, high-efficiency culture of photosynthetic bacteria can also be achieved, and meanwhile the multifunctional airlift tube photobioreactor can be used for microalgae carbon sequestration and wastewater treatment.
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Description

technical field

[0001] The invention belongs to the field of microalgae biotechnology, and relates to a reactor main body and a multifunctional air-lift type tube photobioreactor. Background technique

[0002] Microalgae are rich in protein, polysaccharides, pigments, polyunsaturated fatty acids and multivitamins. At the same time, microalgae have the ability to absorb N / P and accumulate oil. Therefore, microalgae has important application value in many fields such as food, medicine, feed, bait, environmental protection and energy.

[0003] Photobioreactor is the core device of microalgae photoautotrophic culture and light induction (that is, induction of algae cells to synthesize and accumulate useful substances, such as pigments, proteins, etc.) and mixed nutrition culture by light. At present, photobioreactors are mainly divided into open type and closed type. Open photobioreactors mainly refer to raceway ponds and round ponds, which are currently the most widely used c...

Examples

Embodiment 1

[0115] Example 1: Comparison of photoautotrophic culture of Chlorella in airlift tube photobioreactors and single column and horizontal tube photobioreactors.

[0116] The 250L air-lift tube photobioreactor is composed of 12 plexiglass pipes with an inner diameter of 100mm, the distance between the outer wall of the pipe and the outer wall is 50mm, and the top is a square tank with a length of 1.8m, a width of 14cm, and a height of 15cm. The bottom is a pipe with a diameter of 14cm and a length of 1.8m. Ventilate with 1 pipe at intervals, the ventilation volume is 0.2vvm, and the gas contains 2% CO 2 To maintain a pH of around 7.0.

[0117] Sixteen 18L column reactors (height: 2m) with an inner diameter of 100mm (16L liquid content) are arranged in a row, and the distance between the column reactors is 5cm to form a microalgae cultivation system with a total volume of 250L. The bottom of the column reactor is ventilated, the ventilation rate is 0.2vvm, and the gas contains 5...

Embodiment 2

[0121] Example 2: Comparison of photoinduction of Haematococcus pluvialis in an airlift tube photobioreactor and a single column and horizontal tube photobioreactor.

[0122] The 250L air-lift tube photobioreactor is composed of 12 plexiglass pipes with an inner diameter of 100mm. The distance between the outer wall of the pipe and the outer wall is 50mm. It is a pipe with a diameter of 14cm and a length of 1.8m. Ventilate with 1 tube at intervals, the ventilation volume is 0.2vvm, and the gas contains 1% CO 2 To maintain a pH of around 7.0.

[0123] Sixteen 18L column reactors (2.2m high) with an inner diameter of 100mm (with a liquid content of 16L) are arranged in a row, and the distance between the column reactors is 50mm to form a microalgae cultivation system with a total volume of 250L. The bottom of the column reactor is ventilated, the ventilation rate is 0.2vvm, and the gas contains 3% CO 2 To maintain a pH of around 7.0.

[0124] The 250L horizontal tubular phot...