Cell culture device and method
A cell culture and cell technology, applied in tissue cell/virus culture device, sterilization method, biochemical cleaning device, etc., can solve pollution and other problems, achieve the effect of avoiding pollution and improving operation safety
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Embodiment 1
[0085] A cell culture device, such as figure 1 As shown, it includes: a culture tower 100 and a perfusion system.
[0086] The culture tower 100 includes a first adapter cover 110, a second adapter cover 120, eight supports 130 for cell attachment and growth, eight sealing rings 140 and eight buckle rings 150, the first adapter cover 110, the supports 130 and the second connecting cover 120 are stacked in sequence to form a culture tower 100 with a closed cavity. The first connecting cover 110 and the second connecting cover 120 are respectively provided with a perfusion inlet and a perfusion outlet.
[0087] The above-mentioned first connection cover 110, bracket 130 and second connection cover 120 are sequentially stacked and installed in a specific way as follows:
[0088] like Figure 2-4 As shown, the sealing ring 140 is annular and arranged around the edge of the bracket 130, as Figure 5-11 As shown, the retaining ring 150 is annular, and is arranged around the edge ...
Embodiment 2
[0108] A cell culture device, which is basically the same as the cell culture device of embodiment 1, such as Figure 21 As shown, the difference is:
[0109] The cell culture device of this embodiment also includes a humidification device, an air filter 500 , a cell collection bottle 710 , a cell collection tube 720 , a waste liquid bottle 810 , a waste liquid tube 820 , a cell collection valve 730 and a waste liquid valve 830 .
[0110] Figure 21 Among them, the humidification device provides humidified CO for the culture solution storage device 210 by performing humidification treatment on the culture environment (such as the environment in the cell culture box) where the cell culture device is located. 2 gas. Moreover, in this embodiment, an air filter 500 is provided at the air vent of the culture solution storage device 210 communicating with the outside world.
[0111] understandable, such as Figure 22 As shown, the cell culture device may further include an air p...
Embodiment 3
[0122] A cell culture device, which is basically the same as the cell culture device of embodiment 1, such as Figure 23 As shown, the difference is:
[0123]The cell culture device of this embodiment includes three culture towers 100 , and also includes a humidification device, an air filter 500 , a waste liquid bottle 810 and a waste liquid pipe 820 .
[0124] Figure 23 Among them, the humidification device provides humidified CO for the culture solution storage device 210 by performing humidification treatment on the culture environment (such as the environment in the cell culture box) where the cell culture device is located. 2 gas. Moreover, in this embodiment, air filters 500 are provided at the ventilation openings of the culture solution storage device 210 communicating with the outside world.
[0125] understandable, such as Figure 24 As shown, the cell culture device may further include an air pump 400, and the humidification device 300, the air pump 400, and t...
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