Cell culture device and method

A cell culture and cell technology, applied in tissue cell/virus culture device, sterilization method, biochemical cleaning device, etc., can solve pollution and other problems, achieve the effect of avoiding pollution and improving operation safety

Active Publication Date: 2018-03-30
GUANGZHOU JET BIOFILTRATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Based on this, it is necessary to provide a cell culture device for the above problems, using the cell culture device, cells can be cultured in a closed manner, avoiding the pollution problem that may be caused by open culture; and, the cell culture device Also through the use of scaffolds, more surface area for cell generation is provided in the culture device, achieving the purpose of cultivating cells on a large scale

Method used

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  • Cell culture device and method
  • Cell culture device and method
  • Cell culture device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] A cell culture device, such as figure 1 As shown, it includes: a culture tower 100 and a perfusion system.

[0086] The culture tower 100 includes a first adapter cover 110, a second adapter cover 120, eight supports 130 for cell attachment and growth, eight sealing rings 140 and eight buckle rings 150, the first adapter cover 110, the supports 130 and the second connecting cover 120 are stacked in sequence to form a culture tower 100 with a closed cavity. The first connecting cover 110 and the second connecting cover 120 are respectively provided with a perfusion inlet and a perfusion outlet.

[0087] The above-mentioned first connection cover 110, bracket 130 and second connection cover 120 are sequentially stacked and installed in a specific way as follows:

[0088] like Figure 2-4 As shown, the sealing ring 140 is annular and arranged around the edge of the bracket 130, as Figure 5-11 As shown, the retaining ring 150 is annular, and is arranged around the edge ...

Embodiment 2

[0108] A cell culture device, which is basically the same as the cell culture device of embodiment 1, such as Figure 21 As shown, the difference is:

[0109] The cell culture device of this embodiment also includes a humidification device, an air filter 500 , a cell collection bottle 710 , a cell collection tube 720 , a waste liquid bottle 810 , a waste liquid tube 820 , a cell collection valve 730 and a waste liquid valve 830 .

[0110] Figure 21 Among them, the humidification device provides humidified CO for the culture solution storage device 210 by performing humidification treatment on the culture environment (such as the environment in the cell culture box) where the cell culture device is located. 2 gas. Moreover, in this embodiment, an air filter 500 is provided at the air vent of the culture solution storage device 210 communicating with the outside world.

[0111] understandable, such as Figure 22 As shown, the cell culture device may further include an air p...

Embodiment 3

[0122] A cell culture device, which is basically the same as the cell culture device of embodiment 1, such as Figure 23 As shown, the difference is:

[0123]The cell culture device of this embodiment includes three culture towers 100 , and also includes a humidification device, an air filter 500 , a waste liquid bottle 810 and a waste liquid pipe 820 .

[0124] Figure 23 Among them, the humidification device provides humidified CO for the culture solution storage device 210 by performing humidification treatment on the culture environment (such as the environment in the cell culture box) where the cell culture device is located. 2 gas. Moreover, in this embodiment, air filters 500 are provided at the ventilation openings of the culture solution storage device 210 communicating with the outside world.

[0125] understandable, such as Figure 24 As shown, the cell culture device may further include an air pump 400, and the humidification device 300, the air pump 400, and t...

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PUM

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Abstract

The invention relates to a cell culture device and method, belonging to the technical field of cell culture. The culture device includes: a culture tower, including a first adapter cover, a second adapter cover and at least one support for cell attachment and growth, the first adapter cover, at least one of the supports and the second adapter cover are sequentially stacked Set to form a culture tower with a closed cavity, the first connection cover and the second connection cover are respectively provided with a perfusion inlet and a perfusion outlet; and a perfusion system, including a culture solution storage device, a perfusion tube and a supply for A powered perfusion pump, the culture solution storage device, the perfusion pump and the perfusion inlet are sequentially communicated through the perfusion tube. After cell inoculation, the above-mentioned cell culture device assembles various components to form a complete and airtight culture tower, and adopts a perfusion system to complete the culture, thereby avoiding the possibility of contamination and improving operational safety.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a cell culture device and method. Background technique [0002] In recent years, the technology of tissue regeneration engineering, biomedical engineering, and cell therapy industry has developed rapidly. It has become the current clinical application of cells to obtain a large number of cells, extracellular secretions of cells, and cell therapy products through large-scale cultivation of animal cells and human cells in vitro. important direction. For example, tissue trauma, culture of skin cells after burns, acquisition of new skin, as well as tumor cell therapy, cell beauty, cell weight loss, etc., all require in vitro culture of target cells. [0003] At present, both at home and abroad, cells are cultured in vitro by providing cells with medium and culture devices for in vitro growth, and placing the culture devices in an incubator. Commonly used cell culture devices m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/04
CPCC12M23/38C12M23/46C12M23/48C12M23/58C12M29/00C12M29/04C12M29/10C12M29/26C12M33/00C12M37/04C12M23/06C12M23/24C12M23/50
Inventor 袁建华李慧伦王文江苏嘉良李晓琳陈勇
Owner GUANGZHOU JET BIOFILTRATION CO LTD
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