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Method for detecting peripheral blood circulating tumor cell EGFR of advanced rectal cancer patient

A technology for peripheral blood circulation and colorectal cancer, applied in the field of EGFR detection, can solve the problems of difficulty in evaluating the expression status of EGFR, and achieve the effect of real-time detection

Inactive Publication Date: 2016-05-11
SHANDONG RES INST OF TUMOUR PREVENTION TREATMENT
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Problems solved by technology

Immunohistochemical method uses tumor tissue as the detection object, but for colorectal cancer patients at advanced stage where tissue samples cannot be obtained, it becomes difficult to evaluate the expression status of EGFR

Method used

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  • Method for detecting peripheral blood circulating tumor cell EGFR of advanced rectal cancer patient
  • Method for detecting peripheral blood circulating tumor cell EGFR of advanced rectal cancer patient
  • Method for detecting peripheral blood circulating tumor cell EGFR of advanced rectal cancer patient

Examples

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Embodiment Construction

[0039] Below in conjunction with accompanying drawing and embodiment the present invention is described as follows.

[0040] An example of using this technical method to separate, obtain and identify peripheral blood circulating tumor cells of 8 patients with advanced colorectal cancer (while detecting 8 samples of normal people as negative controls).

[0041] 1. Using a membrane filtration device to separate and obtain CTCs in the peripheral blood of patients with advanced colorectal cancer who cannot obtain tissue samples:

[0042] Collect 5ml of peripheral blood from each sample, dilute the peripheral blood with 45ml diluent (composition: 1mmol / lEDTA+0.1%BSA), and then add 3ml of 4% paraformaldehyde to fix the diluted blood sample for 10 minutes.

[0043] At fixed intervals, assemble the membrane filtration device: as attached figure 1 , figure 2 , image 3As shown, the filter device is composed of a filter 3 , a filter membrane 7 , a blood sample container 2 , a waste ...

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Abstract

The invention discloses a method for detecting a peripheral blood circulating tumor cell EGFR of an advanced rectal cancer patient. CTC in peripheral blood of the advanced rectal cancer patient from whom a tissue specimen cannot be obtained is obtained through separation by means of a membrane filtration device, a thin slice is manufactured through the cell wax block technology, and accordingly the EGFR expression situation of the CTC in the peripheral blood of the advanced rectal cancer patient from whom the tissue specimen cannot be obtained is detected. By means of the technological method, the EGFR expression situation of the CTC in the peripheral blood of the advanced rectal cancer patient from whom the tissue specimen cannot be obtained can be detected without colorectal cancer tissue. The technology belongs to minimal invasion and can achieve real-time detection.

Description

technical field [0001] The invention relates to a new method for detecting EGFR, in particular to a method for detecting EGFR in peripheral blood circulating tumor cells of patients with advanced colorectal cancer. Background technique [0002] For patients with advanced colorectal cancer, targeted combination chemotherapy can significantly prolong the disease-free progression-free survival and overall survival of advanced colorectal cancer. Among the targeted drugs currently used clinically in patients with advanced colorectal cancer. Cetuximab is an IgG1 monoclonal antibody targeting EGFR, which has been clinically used and achieved certain curative effect. [0003] EGFR is a member of the ligand-activated receptor tyrosine kinase erB-B family, which is involved in the growth, proliferation and regeneration of intestinal mucosa, and plays a crucial role in the development of colorectal cancer. EGFR is positively expressed in 50%-85% of colorectal cancer samples, and its ...

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/68G01N33/57419G01N2333/71
Inventor 田禾李胜王振丹
Owner SHANDONG RES INST OF TUMOUR PREVENTION TREATMENT
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