Unlock instant, AI-driven research and patent intelligence for your innovation.
Immuno comb array test paper for detecting antibody of simian MeV (measles virus) as well as preparation method and application
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for detecting test strips and viruses, which is applied in the directions of viruses/bacteriophages, biochemical equipment and methods, and biological testing, etc. The effect of the experiment cycle
Inactive Publication Date: 2016-06-08
海南出入境检验检疫局检验检疫技术中心
View PDF1 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Once an outbreak occurs, it spreads quickly and is difficult to control
Measles virus is a stable, monotypic virus that is difficult to eradicate completely, interfering with experimental studies
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0060] Design and synthesis of embodiment 1SOE-PCR primers
[0061] According to the gene sequence of simian MeV virus, SOE-PCR amplification primers were designed using the principle of overlap extension PCR (SOE-PCR). The primer sequences are as follows:
[0071] The mixture was pre-denatured at 95 °C for 5 min, denatured at 94 °C for 1 min, annealed at 60 °C for 30 s, extended at 72 °C for 1 min, 15 cycles, with a total extension of 10 min at 72 °C.
[0075] The mixture was pre-denatured at 95 °C for 5 min, denatured at 94 °C for 1 min, annealed at 63 °C for 30 s, extended at 72 °C for 1 min, 30 cycles, and a total extension of 10 min at 72 °C.
[0084] pMD18T-MeV and pGEX-4T-1 were digested with EcoRI and XhoI respectively, and the digested target fragments were gel recovered. Connect the recovered target fragments, the connection system is as follows:
[0087] The ligation product was transformed into BL21(DE3)pLysS competent cells, the positive colonies were screened by ampicillin resistance, and the plasmid was extracted for identification by PvuⅠ digestion. The result is as figure 2 shown. The positively identified plasmid was named pGEX-4T-MeV.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention mainly relates to immuno comb array test paper for detecting an antibody of a simian MeV (measlesvirus) as well as a preparation method and an application. A recombinant plasmid pGEX-4T-MeV for establishment of an immuno comb array is shown in the sequence table Seq No.4. An upper primer MeVF in a primer pair for establishment of the recombinant plasmid is shown in the sequence table Seq No.1, a lower primer MeVR1 of the primer pair is shown in the sequence table Seq No.2, and a lower primer MeVR2 of the primer pair is shown in the sequence table Seq No.3. The establishment method comprises the following steps: the SOE (splicing overlapping extension)-PCR (polymerasechain reaction) primer pair is designed according to the nucleotide sequence of the simian MeV, simian MeV genes are amplified through SOE-PCR, and the recombinant plasmid pGEX-4T-MeV is established; finally, the immuno comb array for quickly detecting the antibody in blood or serum of an experimental monkey is prepared with a film chromatographic technique according to the enzyme linked immunosorbent assay principle and has good stability, specificity, sensitivity and repeatability.
Description
technical field [0001] The invention relates to a detection method in the field of biotechnology and the technical field of antigens, in particular to an immune comb detection method for monkey MeV virusantibody and a detection kit thereof. Background technique [0002] Measles is an acute infectious disease of humans and other primates caused by measlesvirus (Measles virus, MeV), and it is one of the diseases that must be strictly controlled in monkey farms. Once an outbreak occurs, its spread is fast and difficult to control. Measles virus is a stable monotype virus, which is difficult to eliminate completely, which interferes with experimental research. In recent years, the number of experimental monkeys in my country has grown rapidly, with a total of more than 200,000. The import and export trade volume of experimental monkeys is increasing year by year. With the increasing requirements for the quality of experimental monkeys in the international market, measles vir...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
