Preparation method of gynura nepalensis total flavones

The technology of total flavonoids of Panax notoginseng and chrysanthemum of Nepal, applied in the field of bioengineering, can solve the problem of low purity of total flavonoids of chrysanthemum of Panax notoginseng, and achieve the effects of remarkable technological progress, mild conditions and simple steps.

Inactive Publication Date: 2016-06-15
SHANGHAI INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Aiming at the above-mentioned technical problems in the prior art, the invention provides a kind of preparation method of the total flavonoids of Panax notoginseng of Nepal, the preparation method of this total flavonoids of Panax notoginseng of Nepal will solve the problem obtained by the preparation method of the prior art The technical problem that the purity of the total flavonoids of Panax notoginseng in Nepal is not high

Method used

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  • Preparation method of gynura nepalensis total flavones
  • Preparation method of gynura nepalensis total flavones
  • Preparation method of gynura nepalensis total flavones

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Experimental program
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Effect test

Embodiment 1

[0022] The determination of total flavonoids is represented by the amount of rutin contained in the sample, and rutin is used as a standard product to make a standard curve: use 80% ethanol as a solvent, and accurately prepare a 0.2mg / ml rutin standard solution for use, and take the above standard solution 0.0, 1.0, 2.0, 4.0, 8.0, 10.0ml are placed in a 25ml volumetric flask, add 80% ethanol to prepare 10ml, first add 1ml of 5% sodium nitrite solution, mix well, let stand for 6min; add 1ml of 10% aluminum nitrate solution, shake well and Stand for 6min; add 10ml of 4% sodium hydroxide solution. Finally, place the scale with 80% ethanol at a constant volume for 15 minutes to measure the absorbance at 510 nm and draw a standard curve:

[0023] Y=0.5205X+0.0237,R 2 =0.9999 (X is the concentration of rutin mg / ml, Y is the absorbance value).

[0024] When measuring the sample, take 10ml sample and carry out according to the rutin assay method.

Embodiment 2

[0026] 1) Weigh 15 g of the dried leaves of Panax notoginseng from Nepal, and pulverize it into a powder with a particle size of 40 mesh;

[0027] 2) Add 450ml of ethanol with a mass percentage concentration of 95% to the Nepal chrysanthemum notoginseng obtained in step (1), stir evenly, and extract with ultrasonic waves for 45min at 60°C, filter after extraction, collect the upper filtrate, and refill it in the filter residue Add 300ml of ethanol with a mass percentage concentration of 95%, stir evenly, and then extract with ultrasonic wave at 60°C for 45min, filter after extraction, collect the filtrate, and combine the filtrate collected twice;

[0028] 3) Concentrate the combined filtrate obtained in step (2) to 20ml under reduced pressure, control the temperature during the concentration process to be 50°C, and the pressure to be -0.08~-0.1Mpa; determine the total flavonoids of the concentrated solution (see Table 2-1 for experimental data ), the extraction rate of flavon...

Embodiment 3

[0033] 1) Weigh 15 g of the dried leaves of Panax notoginseng from Nepal, and pulverize it into a powder with a particle size of 60 mesh;

[0034] 2) Add 300ml of ethanol with a mass percentage concentration of 80% to the Nepalese chrysanthemum notoginseng obtained in step (1), stir well, and extract with ultrasonic waves for 45min at 60°C, filter after extraction, collect the filtrate, and add to the filter residue 200ml of ethanol with a mass percentage concentration of 80%, stir evenly, and then extract with ultrasonic wave at 60°C for 45min, filter after extraction, collect the filtrate, and combine the filtrate collected for the second time;

[0035] 3) Concentrate the combined filtrate obtained in step (2) to 20ml under reduced pressure. During the concentration process, the temperature is controlled at 50°C and the pressure is -0.08~-0.1Mpa; the samples are measured, and the results are shown in Table 2-2. The extraction rate calculation formula shows that the extractio...

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Abstract

The invention provides a preparation method of gynura nepalensis total flavones, which comprises the following steps: weighing dried gynura nepalensis leaves, and crushing the dried gynura nepalensis leaves; adding ethanol into dried gynura nepalensis powder, stirring uniformly, carrying out auxiliary extraction with ultrasonic waves or microwaves at 35-65 DEG C or directly extracting in a water bath, filtering after extracting, and collecting upper filtrate; carrying out vacuum concentration on the filtrate, wherein in the concentration process, the temperature is controlled at 50-65 DEG C and the pressure is controlled at -0.08 to -0.1MPa; determining the extraction rate of the flavones according to a formula; adding the concentrated filtrate into an AB-8 macroporous adsorption resin chromatographic column, and respectively eluting with water and ethanol, wherein the temperature is controlled at 25-38 DEG C and the pressure is controlled at -0.08 to -0.1MPa; and carrying out vacuum concentration on the eluent, and determining the extraction rate of the flavones. The method provided by the invention is simple in step and mild in condition; the environmental pollution is avoided in the production process; and the product can be used for processing drugs and health products.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a Nepalese chrysanthemum notoginseng, specifically a preparation method of Nepalese chrysanthemum notoginseng total flavonoids. Background technique [0002] Gynuranepalensis DC. is a plant of the genus Panax notoginseng in the Compositae family. It is a perennial herb with its aerial parts used as medicine. The plant contains a large number of pharmacologically active alkaloids, terpenes, glycosides, flavonoids, sugars, organic acids and other chemical components, among which flavonoids have obvious hypoglycemic effect. Nepal chrysanthemum notoginseng is mainly distributed in Guizhou, Yunnan and other places in my country. However, there are few studies on the extraction process of active ingredients in Nepalese chrysanthemum notoginseng. Contents of the invention [0003] Aiming at the above-mentioned technical problems in the prior art, the invention provides a kind of preparat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28
CPCA61K36/28A61K2236/15A61K2236/333A61K2236/51A61K2236/53A61K2236/55
Inventor 龚钢明晋丛斌李张宇
Owner SHANGHAI INST OF TECH
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