A method for extracting cyclic (pro-glycoside) dipeptide from bacillus coagulans

A technology of Bacillus coagulans and seeds, which is applied in the field of preparation of cyclic dipeptides, and achieves the effect of simple extraction method, low cost and high purity

Inactive Publication Date: 2019-01-04
HUANGHE S & T COLLEGE
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports of small molecule cyclic dipeptides in the metabolites of Bacillus coagulans. Cyclic dipeptides are diketopiperazines with a stable six-membered ring structure, which exhibit antibacterial, antifungal, anti Viral, anti-tumor, immunosuppressive, neuroprotective, anti-hyperglycemic, anti-malarial and other biological and pharmacological activities

Method used

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  • A method for extracting cyclic (pro-glycoside) dipeptide from bacillus coagulans
  • A method for extracting cyclic (pro-glycoside) dipeptide from bacillus coagulans

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Embodiment 1

[0024] A method for extracting cyclic (pro-glycoside) dipeptide from Bacillus coagulans, comprising the following steps:

[0025] (1) Activation of strains: Inoculate Bacillus coagulans on the plate medium and culture at 37°C for 24 hours to obtain activated strains;

[0026] (2) Primary culture: inoculate the activated strains obtained in step (1) into MRS liquid medium, and culture on a shaker at 150r / min and 37°C for 12 hours to obtain 1L of seed solution;

[0027] (3) Secondary culture: inoculate the seed solution obtained in step (2) into the MRS liquid medium, the volume of the seed solution is 4% of the volume of the MRS liquid medium, at 150r / min, 37°C, shaker Cultivate for 48 hours to obtain 20L of fermented liquid;

[0028] (4) Crude extract preparation: the fermentation liquid prepared in step (3) was concentrated by rotary evaporation to 1 / 4 of the original fermentation liquid volume, and then centrifuged, the supernatant was precipitated with ethanol, filtered an...

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Abstract

The invention discloses a method for extracting cyclo(Pro-Gly)dipeptide from Bacillus coagulans. The method mainly comprises the following steps: carrying out strain activation and culture, obtaining a crude extract, carrying out rough separation on the crude extract by silica gel column chromatography, purifying by recrystallization, carrying out TLC (thin layer chromatography) tracking detection, carrying out nuclear magnetic detection on the obtained compounds, and carrying out contradistinction with documents, thereby obtaining the cyclo(Pro-Gly)dipeptide. The raw material Bacillus coagulans is probiotics which are safe and harmless; and the extraction process is simple and is low in cost. The prepared compounds have high purity.

Description

technical field [0001] The invention belongs to the technical field of preparation of cyclic (pro-glycine) dipeptide, and in particular relates to a method for extracting cyclic (pro-glycine) dipeptide from bacillus coagulans. Background technique [0002] Bacillus coagulans (bacillus coagulans LL1103), also known as spore-forming lactic acid bacteria, not only has the characteristics of lactic acid bacteria producing lactic acid, but also has the characteristics of rich enzyme system and stress resistance, high temperature resistance, bile salt resistance and easy storage of Bacillus. As a rising star of the probiotic family, it has been widely used in aquaculture, animal husbandry, food and medicine industries when it is made into microecological preparations. In addition to the health care function of Bacillus coagulans in maintaining the intestinal microecological balance, it has obvious curative effects on common intestinal diseases such as chronic colitis, diarrhea and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/04C07D487/04C12R1/07
CPCC07D487/04C12P21/02
Inventor 陈林郭庆丰马经纬康文艺
Owner HUANGHE S & T COLLEGE
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