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A qualitative and quantitative detection method for microorganisms in water bodies

A technology of microorganisms and target microorganisms, applied in the biological field, can solve the problems of inaccurate qualitative and quantitative detection of microorganisms, and achieve the effects of fine detection results, high resolution and probability guarantee

Active Publication Date: 2020-06-16
JIANGHAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problem of inaccurate qualitative and quantitative detection of microorganisms in the prior art, the embodiment of the present invention provides a qualitative and quantitative detection method for microorganisms in water bodies

Method used

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  • A qualitative and quantitative detection method for microorganisms in water bodies
  • A qualitative and quantitative detection method for microorganisms in water bodies
  • A qualitative and quantitative detection method for microorganisms in water bodies

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Experimental program
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Embodiment

[0039] The identification of embodiment, triangular lake water body microorganism

[0040] Water bodies can include water in rivers, rivers, lakes, seas, glaciers, snowpack, reservoirs, ponds, etc., and can also include groundwater and water vapor in the atmosphere. The sample to be tested in this embodiment is the water body in the Triangular Lake in Wuhan, Hubei. The purpose of detecting microorganisms in the water body is to monitor the pollution of the water body by human and animal feces through the amount of microorganisms.

[0041] Step 1. Determining the target microbial group in the sample to be tested, target microorganisms and non-target organisms, and reference microorganisms that do not exist in the sample to be tested, the specific method is as follows:

[0042] The number of target microbial groups ≥ 1, and each target microbial group includes ≥ 0 target microorganisms; target microorganisms can be bacteria, viruses, fungi, actinomycetes, rickettsia, mycoplasma,...

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Abstract

The invention discloses a qualitative and quantitative detection method for microorganisms in a water body, which belongs to the field of biotechnology. The method comprises the following steps: determining target microbial groups, target microorganisms and non-target organisms, and reference microorganisms that do not exist in the test sample; designing target microbial groups and target microbial characteristic regions; designing Multiple amplification primers for the characteristic region; after adding reference microorganisms and exogenous nucleic acids to the sample to be tested, extract the nucleic acid of the microorganisms in the sample to be tested; use the designed multiple primers to amplify the microbial nucleic acid, and amplify to obtain characteristic sequencing fragments; Characteristic sequencing fragments qualitatively and quantitatively analyze the microorganisms in the sample to be tested. The present invention does not need to pre-cultivate and proliferate microorganisms, and can perform high-throughput, high-accuracy, and high-resolution detection of multiple known microorganisms in a sample to be tested at one time, and the detection process is simple, fast and standardized.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a qualitative and quantitative detection method for microorganisms in water bodies. Background technique [0002] Water body microorganisms are an indicator of water body quality and an important source of human and animal pathogens. Therefore, accurate qualitative and quantitative detection and monitoring of water body microorganisms is very necessary. [0003] Existing qualitative and quantitative detection technologies for microorganisms in water bodies include morphological counting, chip detection, 16S rRNA sequencing, metagenomic sequencing and real-time quantitative PCR (Polymerase Chain Reaction, polymerase chain reaction). Morphological counting detection requires pre-cultivation of microorganisms, which takes a long time, non-culturable microorganisms cannot be detected, and only one type of microorganism can be detected at a time, the throughput is low, the sampling amount...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6869C12Q1/04C12Q1/06
CPCC12Q1/6869C12Q2537/143C12Q2535/122C12Q2545/114
Inventor 张静方治伟彭海
Owner JIANGHAN UNIVERSITY
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