Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Metabolite marker for diagnosing stable angina pectoris

A technology for stable angina pectoris and metabolic markers, which is applied in the field of biochemistry to achieve the effect of promoting standardization, improving diagnostic convenience, and accurate and reliable results

Active Publication Date: 2016-06-29
CHINA PHARM UNIV
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] No one has used plasma metabolite levels to diagnose coronary heart disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Metabolite marker for diagnosing stable angina pectoris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Screening characterization of differential metabolites in plasma between subjects with normal coronary arteries and patients with stable angina

[0031] 1. Objects and methods

[0032] 1. The source of the specimen

[0033] After obtaining the consent of the patients, the peripheral venous blood plasma of 280 patients with stable angina pectoris and 350 subjects with normal coronary arteries in Jiangsu Provincial People's Hospital from September 2010 to June 2015 were collected, and all patients or subjects with normal coronary arteries underwent coronary angiography confirmed. Patients with normal coronary arteries were age- and sex-matched with patients with stable angina. All patients with stable angina and normal coronary arteries have normal heart, lung, liver, kidney and hematopoietic functions.

[0034] The time of blood collection was in the morning on an empty stomach.

[0035] 2. Main reagents

[0036] Acetonitrile and formic acid (UPLC pure) w...

Embodiment 2

[0057] Example 2: Construction of ROC curve to verify the ability of 6 differential metabolites to diagnose stable angina pectoris

[0058] Receiver operating curve (ROC) method was used for verification, and the ability to diagnose coronary heart disease was judged by the expression levels of differential metabolites in the plasma of patients with stable angina pectoris and normal coronary arteries. The results showed that phosphorylcholine, palmitoylethanolamine, lysophosphatidylcholine (16:0), lysophosphatidylcholine (18:2), lysophosphatidylcholine (18:1), phosphatidylinositol (20 :4 / 0:0) These 6 differential metabolites have a strong ability to diagnose patients with stable angina pectoris and normal coronary arteries individually, and the area under the ROC curve (AUC) is greater than 0.7, which has clinical diagnostic significance; At the time of diagnosis, with the increase of the combined number, the AUC further increased, and the highest value was achieved when all 6 ...

Embodiment 3

[0067] Example 3: Application in Screening for Drugs for Treatment or Alleviation of Stable Angina Pectoris

[0068] The blood plasma of 160 patients with stable angina pectoris before and after drug treatment was detected, and the results showed that: with the increase of the course of drug treatment, the patient's condition was gradually relieved, and the levels of the 6 metabolic markers provided by the present invention in plasma gradually increased. It tends to the level of healthy people, and there is no significant difference (P>0.05) between the plasma levels of healthy people and those after complete cure. This result is consistent with the detection results of coronary angiography. Therefore, the metabolic markers provided by the present invention can be used to screen drugs for treating or alleviating stable angina.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a metabolite marker for diagnosing stable angina pectoris. The metabolite marker comprises one or more of phosphorylcholine, palmitoylethanolamine, lysophosphatidylcholine (16:0), lysophosphatidylcholine (18:2), lysophosphatidylcholine (18:1) and phosphatidylinositol (20:4 / 0:0). When the single metabolite marker is used for diagnosing the stable angina pectoris and distinguishing a patient having the stable angina pectoris from a person with normal arteria coronaria, area under the curve (AUC) of ROC is greater than 0.7, and the metabolite marker has clinical diagnostic significance; and when a plurality of metabolite markers are used for diagnosis in a combination manner, along with increasing of the number of the combined metabolite markers, AUC is increased further, is maximum when the six metabolite markers are combined completely, and reaches 0.988, and under the optimal cutoff value, sensitivity and specificity are 98.3% and 99.2% respectively. The metabolite marker provided by the invention can accurately diagnose the stable angina pectoris, and is high in accuracy, high in sensitivity and high in specificity.

Description

technical field [0001] The invention belongs to the field of biochemistry, and relates to metabolic markers for diagnosis and classification of coronary heart disease, in particular to a group of metabolic markers for diagnosing stable angina pectoris. Background technique [0002] Coronary heart disease, also known as ischemic heart disease, involves atherosclerosis in the arteries supplying myocardial blood, that is, coronary atherosclerotic lesions cause vascular lumen narrowing or plaque formation or even rupture and complete blockage, resulting in myocardial ischemia and hypoxia. Or necrosis leads to cardiac ischemic disease, which leads to a series of serious cardiovascular events such as clinical angina pectoris and myocardial infarction. Coronary heart disease is the main killer of human health. It has the characteristics of high incidence rate, high disability rate, high recurrence rate, high mortality rate, and many complications. It has become a major disease that...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/025G01N2030/027
Inventor 朱伟齐炼文王士雷范勇
Owner CHINA PHARM UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com