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Method for detecting GABA, Glu, DA, 5-HT and amphetamine type stimulants from serum

A technology for amphetamines and 5-HT, which is applied in the detection of Glu, 5-HT and amphetamine drugs, DA, and GABA in serum, can solve the problems of low sensitivity, poor selectivity, long detection time and the like, and achieves easy sample processing. , Simple operation, rapid detection effect

Active Publication Date: 2016-06-29
BEIJING TONGREN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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Problems solved by technology

The above method has the following problems: HPLC is easy to operate, but has low sensitivity and poor selectivity, and complex and time-consuming sample pretreatment processes such as derivatization or solid phase extraction are often required; electrochemical methods have low sensitivity; ion chromatography-mass spectrometry requires The use of buffer salt ion pairs is often not compatible with mass spectrometers; GC-MS method sample processing is cumbersome, time-consuming and laborious, and the average detection time is long, which is not suitable for large sample detection

Method used

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  • Method for detecting GABA, Glu, DA, 5-HT and amphetamine type stimulants from serum
  • Method for detecting GABA, Glu, DA, 5-HT and amphetamine type stimulants from serum
  • Method for detecting GABA, Glu, DA, 5-HT and amphetamine type stimulants from serum

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example 1

[0095] The establishment of a detection method for GABA, Glu, DA, 5-HT and amphetamines in serum samples of drug addicts includes the following steps:

[0096] (1) Preparation of standard solution:

[0097] ① GABA: Weigh 11.00mg of GABA standard substance, put it in a 10mL volumetric flask, and dissolve it in pure water to obtain GABA1.10mg·mL according to the standard curve and quality control working solution requirements -1The standard stock solution was diluted with pure water to obtain a series of working solutions and quality control working solutions.

[0098] ②Glu: Weigh 21.00mg of Glu into a 10mL volumetric flask and dissolve it in pure water to obtain 2.10mg·mL -1 , according to the need to prepare the corresponding concentration of Glu working solution.

[0099] ③DA and 5-HT: Weigh DA·HCl (11.37mg) and 5-HT·HCl (10.37mg) standard products, put them in a 10mL brown volumetric flask, dissolve with methanol and bring up to the mark. According to the requirements of ...

example 2

[0169] Determination of Serum Samples of Drug Addicts

[0170] The treatment method of serum samples of drug addicts is the same as that of the "blank" serum samples in Example 1 above. Randomly take 25 serum samples of drug addicts. After sample processing and injection of LC-MS / MS to collect data, the serum samples of unknown drug addicts are calculated by the accompanying standard curve. The target compound of the sample (Glu was quantified by the internal standard one-point method), and the calculation results are shown in Table 8.

[0171] Table 8 Calculation results of target compounds in serum samples of drug addicts

[0172]

[0173] BLOQ: The detection value is lower than the lower limit of quantitation; ND: Not detected;

[0174] The results in Table 8 show that MDA, MDMA and MDEA were not detected in the serum of drug addicts, which is consistent with the current situation of amphetamine drug use in China. These three drugs are not common in seized drugs. The s...

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Abstract

The invention discloses a method for detecting GABA, Glu, DA, 5-HT and amphetamine type stimulants from serum. The method comprises steps as follows: S1: preparation of a standard liquid: GABA, Glu, DA, 5-HT and amphetamine type stock solutions, standard curve working solutions and quality control working solutions are prepared, and an internal standard compound stock solution and a working solution are prepared; S2: treatment of blank serum samples and a drug addict serum sample: the standard curve working solutions or the quality control working solutions of GABA, Glu, DA, 5-HT and amphetamine type compounds are added to the blank serum samples respectively, then a trichloroacetic acid containing Glu-d5 and a acetonitrile solution containing DA-d4 and Amp-d6 are added sequentially, vortex shocking and centrifugation are performed, and supernatants are sucked for testing; except addition of the standard curve working solutions or quality control working solutions, an unknown biological sample is subjected to the treatment manners the same as above; S3: liquid chromatograph-mass spectrometer detection: the supernatants prepared in the S2 are detected; S4: drawing of standard curves. The method has the characteristics of convenience in operation, high efficiency and practicability.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, specifically to the field of pharmacokinetics and biological sample content determination, and in particular to a detection method for GABA, Glu, DA, 5-HT and amphetamine drugs in serum. Background technique [0002] Represented by Methamphetamine (MA for short, commonly known as: methamphetamine, other amphetamine compounds include Amp; New drugs are becoming the most widely abused, fastest spreading and most harmful drugs in the world. Studies have shown that methamphetamine can damage central nervous cells. In certain brain regions, the abusers will experience a decrease in the levels of dopamine and 5-hydroxytryptamine, a decrease in the activity of tyrosine hydroxylase and tryptophan hydroxylase, and a decrease in the activity of dopamine and 5-hydroxytryptophan. The decreased absorption and transport capacity of serotonin and the histologically visible terminal damage suggest ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/88
CPCG01N30/02G01N30/06G01N30/88G01N2030/027G01N2030/8822
Inventor 赵秀丽
Owner BEIJING TONGREN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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