Method for discriminating soybeans with different stress-tolerant potential based on metabonomics measure

A metabolomics and potential technology, applied in the field of analytical chemistry, can solve the problems of difficult to achieve the expected purpose, unreasonable evaluation, cumbersome operation, etc., and achieve the effect of reliable data, accurate measurement, and simple and convenient operation

Active Publication Date: 2016-07-20
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In the above two cases, the evaluation process of soybean resources is time-consuming and labor-intensive. If the number of evaluation objects is too large, the operation will be more cumbersome; and the evaluation may be unreasonable and untrue due to special climate reasons, or it may be more difficult to achieve expectations Objective, in practice, there is an urgent need for a method that can quickly and efficiently evaluate the stress tolerance potential of soybean

Method used

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  • Method for discriminating soybeans with different stress-tolerant potential based on metabonomics measure
  • Method for discriminating soybeans with different stress-tolerant potential based on metabonomics measure
  • Method for discriminating soybeans with different stress-tolerant potential based on metabonomics measure

Examples

Experimental program
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Effect test

example 1

[0066] The soybean samples to be tested in this example include 9 soybean germplasm resources collected in southwestern regions such as Sichuan, Chongqing, and Guangxi, including wild resources, typical varieties commonly used in southwest soybean production and unapproved breeding intermediate materials. Soybean seed sample information is as follows Table 1 shows.

[0067] Table 1 Material information of 9 kinds of soybeans with different resistance to field mold in Example 1

[0068]

[0069] First, the samples were collected and crushed and passed through a 60-mesh sieve, and then the obtained soy flour was stored in an ultra-low temperature refrigerator at -80°C for later use.

[0070] Secondly, the analysis steps of soy flour for each sample are as follows:

[0071] 1) Accurately weigh 200.0mg of soy flour, place it in a 10mL centrifuge tube with a lid, add 5mL of methanol aqueous solution with a volume concentration of 80% (control the material-to-liquid ratio to 40:1); after se...

example 2

[0080] The soybean samples to be tested in this example are all from all parts of Sichuan, including 6 local wild germplasm resources, 1 common cultivated variety (Nandou 12), 1 cultivated variety (Gongxuan No. 5) in the field, and soybean seeds The sample information is shown in Table 3.

[0081] Table 3 Material information of 8 kinds of soybeans with different shade tolerance characteristics in Example 2

[0082]

[0083]

[0084] First, the samples are collected and crushed and passed through a 60-mesh sieve, and then the obtained soy flour is stored in an ultra-low temperature refrigerator at -60°C for later use.

[0085] Secondly, the analysis steps of soy flour for each sample are as follows:

[0086] 1) Accurately weigh 200.0mg of soy flour, place it in a 10mL centrifuge tube with a lid, add 5mL of methanol aqueous solution with a volume concentration of 80% (control the material-to-liquid ratio to 40:1); after sealing, place it in a vortex shaker 10s; then ultrasonic (40KHz,...

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Abstract

The invention discloses a method for discriminating soybeans with different stress-tolerant potential based on a metabonomics measure. According to the method for discriminating the soybeans with the different stress-tolerant potential based on the metabonomics measure, the content of soybean isoflavone in the extract of a soybean seed is analyzed to obtain a soybean isoflavone target metabolic map by adopting a high-performance liquid chromatography-mass spectrometry technique; subsequently, a soybean germplasm resource with the stress-tolerant potential is discriminated by utilizing the obtained absolute content of the soybean isoflavone in the soybean seed; a reference is provided for evaluating the stress tolerance of the soybean and selectively breeding a high-quality soybean variety is provided. The method for discriminating the soybeans with the different stress-tolerant potential based on the metabonomics measure is simple and quick to operate and reliable in result, and can be used for providing a reference for judging and screening the high-quality soybean germplasm resource with the stress-tolerant potential.

Description

Technical field [0001] The present invention relates to the technical field of analytical chemistry, and more specifically, to a method for distinguishing soybeans with different stress tolerance potentials based on metabonomics. Background technique [0002] The evaluation and selection of crop wild germplasm resources are the basis for the selection of high-quality varieties. It is extremely important to carry out the evaluation of agronomic and quality traits according to the direction of crop breeding. The use of reasonable and effective evaluation methods can greatly reduce the selection of high-quality varieties. The process of education. [0003] Take the selection of field mildew-resistant soybeans and high-quality soybean varieties with shade tolerance as an example: 1) Southern summer soybeans are harvested during continuous rainy and humid weather, with serious seed mildew, large yield losses, endangering food safety, and resistance to field mildew The selection and bre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/89
CPCG01N30/02G01N30/06G01N30/89G01N2030/042
Inventor 刘江刘卫国张静邓俊才吴海军杨峰舒凯杜俊波王小春雍太文杨文钰
Owner SICHUAN AGRI UNIV
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