Method and matrix for breeding hybrid embryos of peach trees by aid of test tubes on basis of one-step seedling formation

Abstract

The invention discloses a method and a matrix for breeding hybrid embryos of peach trees by the aid of test tubes on the basis of one-step seedling formation. The method includes acquiring sterile hybrid embryos, and to be more specific, disinfecting and treating seed embryos of seeds of the fruit trees to obtain samples of the sterile hybrid embryos; cultivating the sterile hybrid embryos in the test tubes filled with cultivation substrates to obtain embryo-germination plants which are test tube plants; acquiring plant seedlings outside the test tubes, to be more specific, transplanting the test tube plants into seedling growing substrates outside the test tubes when a plurality of lateral roots grow on radicals of the test tube plants and new shoots with the lengths longer than 10 cm grow on most embryos of the test tube plants, and cultivating the test tube plants to obtain plants outside the test tubes. The method and the matrix have the advantages that the embryo-germination test tube plants can be cultivated in the cultivation substrates in the test tubes, the plants which can normally grow in outdoor environments can be cultivated in the seedling growing substrates outside the test tubes, accordingly, the seeds of the fruit trees no longer need to be directly sowed, and the emergency rate of the seeds of the hybrid peach trees can be increased.

Description

1. Technical field [0001] The invention relates to a method for cultivating peach tree hybrid embryos in test tubes and a cultivating substrate, in particular to a method for cultivating peach tree hybrid embryos in test tubes and a cultivating substrate based on one-time seedling formation. 2. Background technology [0002] Embryo culture of plants plays an important role in overcoming early abortion of distant hybrid embryos, embryo rescue, induction of somatic embryos, and rapid propagation of rootstocks, especially in drupe fruit crops such as peaches, apricots, and cherries. For early-maturing varieties, with the help of embryo culture technology, young embryos, i.e. immature embryos, can be successfully cultivated into seedlings, which is the basis for the cultivation of early-maturing stone fruit tree varieties. Biological breeding method and breeding substrate. In the existing one-time seedling-based hybrid fruit tree embryo test tube cultivation method and cultivati...

AI Technical Summary

Problems solved by technology

[0002] Embryo culture of plants plays an important role in overcoming early abortion of distant hybrid embryos, embryo rescue, induction of somatic embryos, and rapid propagation of rootstocks, especially in drupe fruit crops such as peaches, apricots, and cherries. For early-maturing varieties, with the help of embryo culture technology, young embryos, i.e. immature embryos, can be successfully cultivated into seedlings, which is the basis for the cultivation of early-maturing stone fruit tree varieties. Biological breeding method and breeding substrate. In the existing one-time seedling-based hybrid fruit tree embryo test tube cultivation method and cultivation substrate, because the embryos of early-maturing fruit tree varieties are usually underdeveloped, conventional seeding cannot emerge or the germination rate is very low. The use of embryo culture technology can overcome the problem of seedlings not emerging, but some hybrids for fruit tree cross-breeding for quality or resistance improvement, even if the hybrid embryos have matured, conventional sowing can not emerge or the emergence rate is very low, this problem Seriously hindered the breeding process of fruit tree variety improvement

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