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Molecular marker and method for identifying allelotype of rice chalk gene Chalk5

A technology of molecular markers and alleles, applied in the field of biotechnology engineering, can solve problems such as inaccurate identification, achieve accurate operation and improve predictability

Inactive Publication Date: 2016-09-07
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] The present invention aims at the technical problem that the rice chalkiness rate is continuously distributed in the process of rice appearance quality improvement and breeding and is easily affected by climate and other environmental conditions, resulting in inaccurate identification.

Method used

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  • Molecular marker and method for identifying allelotype of rice chalk gene Chalk5
  • Molecular marker and method for identifying allelotype of rice chalk gene Chalk5
  • Molecular marker and method for identifying allelotype of rice chalk gene Chalk5

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Embodiment Construction

[0023] In order to fully disclose a molecular marker and a method thereof for identifying rice chalkiness gene Chalk5 alleles of the present invention, the method verification and implementation examples are described below. Its specific implementation steps are as follows:

[0024] (1) Test materials

[0025] All rice production promotion varieties: Nanjing 1, Nanjing 11, Nanjing 14, Nanjing 15, Nanjing 16, BG90-2, Gui 630, Sunong 3037, Minghui 78, 9311, IRBB21, Guangluai 4 No., Yuejingsimiao, Zhehui 7954, Lehui 188, Guanghui 128, IR64, Bantau Nante, Shuhui 527, Duohui 1, Mianhui 725, II-32B, Yuetai B, Huafeng B , Lianjing 4, Wuyunjing 21, Huaidao 11, Huajing 6, Yanjing 11, Lianjing 6, Lianjing 7, Huaruidao 1, Lianjing 11, Wuyunjing 27. Huaidao 14, Sidao 785, Huainuo 12, Tongnuo 3, Yangnongdao 1, Nanjing 45, Ningjing 5, Zhendao 14, Yangyujing 2, Sudao 5 No., Nanjing 49, Ning 9108, Yangjing 805, Zhendao 10, Minghui 63 and Nipponbare. The above materials are all publicly kn...

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Abstract

The invention relates to a molecular marker for identifying an allelotype of a rice chalk gene Chalk5. Two pairs of specific PCR (polymerase chain reaction) primer molecular markers, namely Chalk12 and NChalk12 as well as Chalk22 and NChalk22, are developed in accordance with functional mutant sites of dominant and recessive alleles of a major gene Chalk5 which is capable of significantly relieving rice chalk so as to improve the appearance quality of rice. In addition, the invention relates to a method for identifying the allelotype of the rice chalk gene Chalk5, wherein DNAs of various rice plants are amplified by virtue of the molecular marker. The molecular marker and the method disclosed by the invention not only can accurately identify whether the rice contains the rice chalk gene Chalk5 or not but also can further distinguish homozygote and heterozygote of the Chalk5 gene, so that the selection efficiency of rice grain weight is improved.

Description

technical field [0001] The invention relates to the field of biotechnology engineering, in particular to a molecular marker and a method for discriminating rice chalky gene Chalk5 allele type, which belongs to the field of biotechnology engineering and is used for the identification of Chalk5 allele type rice germplasm resources and varieties breeding. Background technique [0002] Rice is the main food crop in my country, and its planting area and total output both rank first in my country. China leads the world in hybrid breeding, flower breeding and utilization of heterosis. With the improvement of people's living standards, consumers' demand for rice quality is getting higher and higher. Rice quality consists of processing quality, appearance quality, cooking and eating quality, and nutritional quality. The specific contents include: transparency, chalkiness, grain shape, amylose content, gel consistency, gelatinization temperature, protein content, and aroma, etc. Am...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 朱金燕杨杰王军李文奇范方军王芳权仲维功
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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