LGR4 gene detection kit

A gene detection and kit technology, applied in the field of LGR4 gene detection kits, to achieve high sensitivity

Inactive Publication Date: 2016-09-14
SHANGHAI INST FOR ENDOCRINE & METABOLIC DISEASES +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, few research groups have studied the functions of LGR4 in nutrition, metabolism, etc.

Method used

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Experimental program
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Effect test

Embodiment 1

[0021] 1. Tissue total RNA extraction

[0022] (1) Tissue homogenate: Take about 20-30mg of each liver and add 1mL TRIZOL reagent to lyse. Homogenize with a homogenizer in an ice bath, transfer the sample to a 1.5mL centrifuge tube, and place at room temperature for 5min.

[0023] (2) Liquid phase separation: Operate in a special fume hood for RNA extraction, add chloroform at a ratio of 0.2mL / mL Trizol, shake vigorously and mix well, place at room temperature for 2-3min, and then centrifuge at 12000g for 15min at 4°C.

[0024] (3) RNA precipitation: Carefully transfer the upper aqueous phase to a new 1.5mL centrifuge tube, add isopropanol at a ratio of 1:1, leave it at room temperature for 10min, then centrifuge at 12000g, 4°C for 15min.

[0025] (4) RNA rinsing: After centrifugation, a white precipitate appeared at the bottom of the tube, remove the supernatant, rinse the RNA precipitate with 75% ethanol (made in DEPC water), centrifuge at 7500g, 4°C for 5min.

[0026] (5)...

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Abstract

The invention relates to an LGR4 gene detection kit. The kit comprises the following primer pairs: m36b4, mPEPCK, mG6pase, mHNF4a, mChREBP, mSREBP1c, mFAS, mSCD1, and mLGR4. The LGR4 gene detection kit has a high detection sensitivity. Through the detection of the kit, key gene expression and related pathways of glycometabolism in the liver can be verified, and clinical individual treatment for patients, prognosis, and prevention intervention becomes more convenient.

Description

technical field [0001] The invention belongs to the field of type 2 diabetes, and in particular relates to an LGR4 gene detection kit. Background technique [0002] In recent years, due to reasons such as obesity, unhealthy diet and lifestyle, and population aging, the incidence of diabetes in various countries in the world has increased sharply, and its mortality rate is second only to cancer and cardiovascular disease, becoming the third largest type of disease that threatens human health. According to the latest data from the International Diabetes Federation (IDF), there were 380 million diabetic patients worldwide in 2014; type diabetic patients. In my country, the incidence of diabetes is also increasing day by day, and the incidence of diabetes tends to be younger. Type 2 diabetes has become one of the important diseases that seriously threaten human health and quality of life. [0003] Insulin resistance in the liver is an important driving factor leading to type ...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/118C12Q2600/158
Inventor宁光张翼飞张志国姚霜霜王计秋
OwnerSHANGHAI INST FOR ENDOCRINE & METABOLIC DISEASES