Application of cryptotanshinone combined with curcumin in the preparation of tumor therapeutic drugs

A technology of cryptotanshinone and curcumin, which is applied in the application field of preparing tumor therapeutic drugs, and can solve problems such as strong side effects, retardation of proliferation, and poor targeting

Active Publication Date: 2020-01-14
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects and deficiencies of existing antitumor drugs, mainly due to the poor targeting of chemotherapy regimens (that is, no distinction between normal cells and cancerous cells, and mandatory blockage of the proliferation of all cells in the body), strong side effects , high cost and other disadvantages, provide a combination of two active ingredients of Chinese herbal medicines with different anti-cancer mechanisms—Cryptotanshinone (CPT for short) and Curcumin (Cur for short). Significant synergistic effect between two compounds to achieve the purpose of killing tumor cells with low dose and high efficiency. At the same time, due to the low toxicity of Chinese herbal medicine, rich sources and easy access to materials, it can also greatly reduce the toxic and side effects of drugs on the body during treatment and reduce Significantly lower treatment costs

Method used

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  • Application of cryptotanshinone combined with curcumin in the preparation of tumor therapeutic drugs
  • Application of cryptotanshinone combined with curcumin in the preparation of tumor therapeutic drugs
  • Application of cryptotanshinone combined with curcumin in the preparation of tumor therapeutic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1 The study of combination therapy of cryptotanshinone and curcumin in colon cancer cell lines

[0082] A certain concentration gradient of cryptotanshinone and curcumin were used to treat colon cancer HCT-116 cells for 24 hours, while the concentration of curcumin was fixed, and different gradient concentrations of cryptotanshinone were used to treat colon cancer HCT-116 cells for 24 hours. The method was used to calculate the inhibitory intensity of cell proliferation under three different drug treatment modes.

[0083] The specific research methods are as follows:

[0084] 1. Experimental cell lines

[0085]HCT-116 colon cancer cells were obtained from the Bioengineering Research Center of Sun Yat-sen University (Guangdong, Guangzhou).

[0086] 2. Cell culture:

[0087] HCT-116 cells were cultured with complete medium (DMEM medium containing 10% fetal bovine serum and 1% double antibody) at a constant temperature of 37°C and 5% CO 2 conditions.

[0088] ...

Embodiment 2

[0106] Example 2 The study of combination therapy of cryptotanshinone and curcumin in chronic myelogenous leukemia cell lines

[0107] Select a certain concentration gradient of cryptotanshinone and curcumin to treat chronic myelogenous leukemia K562 cells for 24 hours, and at the same time fix the concentration of curcumin and use different gradient concentrations of cryptotanshinone to treat leukemia K562 cells for 24 hours. The respective strengths of proliferation inhibition under the three different drug treatment modes.

[0108] The specific research methods are as follows:

[0109] 1. Experimental cell lines

[0110] K562 chronic myelogenous leukemia cells were obtained from the Bioengineering Research Center of Sun Yat-sen University (Guangdong, Guangzhou).

[0111] 2. Cell culture:

[0112] K562 cells were cultured with complete medium (RPMI-1640 medium containing 10% FBS and 1% double antibody) for routine cell culture at a constant temperature of 37°C and 5% CO ...

Embodiment 3

[0131] Example 3 Combination of cryptotanshinone, curcumin and oridonin in colon cancer cell lines

[0132] A certain concentration of cryptotanshinone, curcumin and oridonin were used to treat colon cancer HCT-116 cells for 24 hours, and at the same time, the three drugs were used in combination to treat colon cancer HCT-116 cells for 24 hours, and the MTT colorimetric method was used to calculate the concentration of the cells. The respective strengths of inhibition of proliferation in several different drug treatment modes.

[0133] The specific research methods are as follows:

[0134] 1. Experimental cell lines

[0135] HCT-116 colon cancer cells were obtained from the Bioengineering Research Center of Sun Yat-sen University (Guangdong, Guangzhou).

[0136] 2. Cell culture:

[0137] HCT-116 cells were cultured with complete medium (DMEM medium containing 10% fetal bovine serum and 1% double antibody) at a constant temperature of 37°C and 5% CO 2 conditions.

[0138] 3....

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Abstract

The invention discloses application of a combination of cryptotanshinone and curcumin in preparation of a drug for treating a tumor and application of a combination of oridonin, cryptotanshinone and curcumin in preparation of a drug for treating the tumor. Research finds that by conducting drug combination through quantitative compatibility on the Chinese herb effective ingredients with the different anticancer mechanisms, the significant synergistic interaction effect can be generated. Through the two compatibility modes, more tumor cells can be killed by the pharmaceutical combination with the low concentration, the total drug concentration obtained when the tumor cell proliferation inhibition rate of the drug reaches 50% can be effectively decreased, and the dosages of all the drugs in the compatibility modes are not within the range that hurt can be caused to the organism. Accordingly, the two drug combination schemes have the important significance and the wide application prospect both on preparation of the antitumor drugs and on tumor prevention, treatment and research.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of cryptotanshinone combined with curcumin in the preparation of tumor treatment drugs. Background technique [0002] Tumor is a malignant disease that seriously threatens the survival and health of human beings. Currently, the treatment of tumors mainly focuses on the following programs: surgery, chemotherapy, radiotherapy and immunotherapy, etc. Among them, chemotherapy has become the mainstream postoperative treatment because of its relatively strong targeting and relatively low treatment cost. [0003] In the commonly used chemotherapy regimens, the idea of ​​drug combination has been fully reflected. At present, most of the commonly used drug combination methods in clinic are western medicine combined with western medicine and western medicine combined with traditional Chinese medicine, and most of the western medicines used are cell cy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/9066A61K31/58A61K45/06A61P35/00A61P35/02A61P37/04A23L33/00A61K31/12A61K31/352
CPCA61K31/12A61K31/352A61K31/58A61K36/53A61K36/537A61K36/9066A61K45/06A61K2300/00
Inventor 董博文周惠屈良鹄
Owner SUN YAT SEN UNIV
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