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Preparation of novel nucleic acid dye for polyacrylamide gel electrophoresis

A polyacrylamide gel, nucleic acid dye technology, applied in the field of fluorescent dyes, can solve problems such as incompatibility, and achieve the effects of high sensitivity, novel design and high stability

Inactive Publication Date: 2016-10-12
苏州宇嘉品恒企业管理咨询有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this dye can only be used on 254nm-based UV gel scanners, not compatible with 488nm-based UV gel scanners

Method used

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  • Preparation of novel nucleic acid dye for polyacrylamide gel electrophoresis
  • Preparation of novel nucleic acid dye for polyacrylamide gel electrophoresis
  • Preparation of novel nucleic acid dye for polyacrylamide gel electrophoresis

Examples

Experimental program
Comparison scheme
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Embodiment

[0055] Such as figure 1 Shown, a kind of novel nucleic acid dye that is used for polyacrylamide gel electrophoresis, is characterized in that, is divided into following four-step reaction and obtains altogether:

[0056] 1. The first step reaction

[0057] 1.1 Reaction equation

[0058]

[0059] in:

[0060] 4-Methylquinoline

[0061] 6-bromohexanoic acid

[0062] for intermediate I

[0063] 1.2 Reaction operations

[0064] Add 4-methylquinoline and 6-bromohexanoic acid into a 100mL two-necked bottle, seal it with a rubber stopper, heat at 130°C for 3h, and then raise the temperature to 150°C for 12h. TLC tracking reaction, developer: 15% MeOH / CH 2 Cl 2 . The hot reaction solution was poured into 150 mL of acetonitrile, stirred, and 100 mL of ethyl acetate was added, and a solid precipitated out, which was filtered. Add 150 mL of ethyl acetate to the obtained solid, and stir for 2 h. After filtration, the solid was dried to obtain Intermediate I.

[0065] Th...

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Abstract

The invention provides preparation of a novel nucleic acid dye for polyacrylamide gel electrophoresis. Following reactions are sequentially conducted:, wherein is 4-methylquinoline, and is 6-bromohexanoic acid; , wherein is 2-(methylmercapto)benzothiazole, and is methyl p-toluenesulfonate; , wherein DMF is N,N-dimethyl formamide, Et3N is triethylamine, and HCl(conc.) is concentrated hydrochloric acid; , wherein is 2-succinimido-1,1,3,3-tetramethyluronium tetrafluoroborate (TSTU), DIPEA is N, N-diisopropyl ethylamine, is taurine, and is a product compound. Improvement is conducted on a molecular structure, therefore, the nucleic acid dye can easily penetrate into a high-density high polymer, the high sensitivity and high stability of the nucleic acid dye are kept, and the technical problem that a fluorescent dye serving as a nucleic acid gel dye is difficult to penetrate into the high polymer and the problem that the nucleic acid dye generally has the high toxicity are solved.

Description

technical field [0001] The invention relates to the field of fluorescent dyes, in particular to the preparation of a novel nucleic acid dye for polyacrylamide gel electrophoresis. Background technique [0002] Existing safe and non-toxic dyes used in agarose gels, such as gelgreen dyes produced by Biotium Corporation in the United States, cannot be used in polyacrylamide gel electrophoresis due to their large molecular weight. The reason is that polyacrylamide gel is a denser three-dimensional high polymer. This highly dense polymer structure makes it difficult for fluorescent dyes as nucleic acid gel dyes, especially macromolecules, to penetrate in when separating nucleic acid molecules of different size ranges and different concentration ranges, resulting in poor nucleic acid staining effects. [0003] Common gel imagers used for nucleic acid electrophoresis are generally divided into two bands, namely ultraviolet and gel laser scanners around 488nm. Gel electrophoresis ...

Claims

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Application Information

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IPC IPC(8): C07D417/06C09B57/00C09K11/06G01N1/30G01N27/447
CPCC07D417/06C09B57/00C09K11/06C09K2211/1029G01N1/30G01N27/447
Inventor 夏继波聂承斌钱近春赵育明赵文俊
Owner 苏州宇嘉品恒企业管理咨询有限公司
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